Lutengano W Mwanginde1,2, Mtebe Majigo1, Debora C Kajeguka3, Agricola Joachim1. 1. Department of Microbiology and Immunology, Muhimbili University of Health and Allied Sciences, P. O. Box 65001, Dar es Salaam, Tanzania. 2. Directorate of Forensic Bureau, Ministry of Home Affairs, P. O. Box 9094, Dar es Salaam, Tanzania. 3. Faculty of Medicine, Kilimanjaro Christian Medical University College, P. O. Box 2240, Moshi Kilimanjaro, Tanzania.
Abstract
BACKGROUND: Bacteria possessing extended-spectrum beta-lactamase (ESBL), especially E. coli and Klebsiella species, are problematic, particularly in hospitalized patients. Poultry meat vendors are at risk of carrying ESBL-producing bacteria when processing and handling meat products in an unhygienic environment. There is limited information on the carriage rate of ESBL-producing pathogens among poultry meat vendors that necessitated the conduction of the study. METHOD: A cross-sectional study was conducted among poultry meat vendors in Dar es Salaam, Tanzania. Participants provided rectal swabs in transport media upon instruction. The primary isolation of ESBL-producing bacteria was carried out using MacConkey agar supplemented with ceftazidime. Identification of isolates relied on conventional methods. Double-disk synergy was the method used to confirm ESBL-producing isolates. We performed descriptive statistics using Statistical Package for Social Sciences version 23. A p value < 0.05 was considered statistically significant. RESULTS: A total of 300 participants were recruited from five districts, with a mean age of 27.2 ± 6.7 years. The majority was male (67.3%), and 74.7% worked as poultry meat vendors for more than one year. Out of 300 participants, 107 (35.7%) had confirmed ESBL-producing E. coli and Klebsiella spp. The majority of confirmed ESBL-producing isolates was E. coli (78.5%). Participants from Ubungo District had significantly higher carriage of ESBL-producing Escherichia coli and Klebsiella spp. (48.0%, 95% CI: 34.8-47.7) than Temeke District (21.4%, 95% CI: 13.4-32.4). Only 28.0% of participants had access to latrines at the workplace, and all working areas lacked access to running water. CONCLUSION: The study revealed a relatively high fecal carriage rate of ESBL-producing E. coli and Klebsiella spp. among poultry meat vendors. Poor working environments and hygienic practices are risks for spread of these multidrug-resitant pathogens.
BACKGROUND: Bacteria possessing extended-spectrum beta-lactamase (ESBL), especially E. coli and Klebsiella species, are problematic, particularly in hospitalized patients. Poultry meat vendors are at risk of carrying ESBL-producing bacteria when processing and handling meat products in an unhygienic environment. There is limited information on the carriage rate of ESBL-producing pathogens among poultry meat vendors that necessitated the conduction of the study. METHOD: A cross-sectional study was conducted among poultry meat vendors in Dar es Salaam, Tanzania. Participants provided rectal swabs in transport media upon instruction. The primary isolation of ESBL-producing bacteria was carried out using MacConkey agar supplemented with ceftazidime. Identification of isolates relied on conventional methods. Double-disk synergy was the method used to confirm ESBL-producing isolates. We performed descriptive statistics using Statistical Package for Social Sciences version 23. A p value < 0.05 was considered statistically significant. RESULTS: A total of 300 participants were recruited from five districts, with a mean age of 27.2 ± 6.7 years. The majority was male (67.3%), and 74.7% worked as poultry meat vendors for more than one year. Out of 300 participants, 107 (35.7%) had confirmed ESBL-producing E. coli and Klebsiella spp. The majority of confirmed ESBL-producing isolates was E. coli (78.5%). Participants from Ubungo District had significantly higher carriage of ESBL-producing Escherichia coli and Klebsiella spp. (48.0%, 95% CI: 34.8-47.7) than Temeke District (21.4%, 95% CI: 13.4-32.4). Only 28.0% of participants had access to latrines at the workplace, and all working areas lacked access to running water. CONCLUSION: The study revealed a relatively high fecal carriage rate of ESBL-producing E. coli and Klebsiella spp. among poultry meat vendors. Poor working environments and hygienic practices are risks for spread of these multidrug-resitant pathogens.
Authors: James Cohen Stuart; Thijs van den Munckhof; Guido Voets; Jelle Scharringa; Ad Fluit; Maurine Leverstein-Van Hall Journal: Int J Food Microbiol Date: 2012-01-03 Impact factor: 5.277
Authors: Stephen E Mshana; Lisa Gerwing; Mercy Minde; Torsten Hain; Eugen Domann; Eligius Lyamuya; Trinad Chakraborty; Can Imirzalioglu Journal: Int J Antimicrob Agents Date: 2011-07-12 Impact factor: 5.283
Authors: Sarah Tschudin-Sutter; Reno Frei; Roger Stephan; Herbert Hächler; Danica Nogarth; Andreas F Widmer Journal: Infect Control Hosp Epidemiol Date: 2014-05 Impact factor: 3.254
Authors: Frieder Schaumburg; Abraham S Alabi; Lisa Frielinghaus; Martin P Grobusch; Robin Köck; Karsten Becker; Saadou Issifou; Peter G Kremsner; Georg Peters; Alexander Mellmann Journal: BMC Microbiol Date: 2014-11-19 Impact factor: 3.605
Authors: Eric G Evers; Annemarie Pielaat; Joost H Smid; Engeline van Duijkeren; Francy B C Vennemann; Lucas M Wijnands; Jurgen E Chardon Journal: PLoS One Date: 2017-01-05 Impact factor: 3.240