Xiangqi Fan1, Haiyan Hu1, Daiwen Chen1, Bing Yu1, Jun He1, Jie Yu1, Junqiu Luo1, Erik Eckhardt2, Yuheng Luo1, Jianping Wang1, Hui Yan1, Xiangbing Mao3. 1. Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Key laboratory of Animal Disease-resistant Nutrition and Feed of China Ministry of Agriculture and Rural Affairs, Key laboratory of Animal Disease-resistant Nutrition of Sichuan Province, Institute of Animal Nutrition, Sichuan Agricultural University, No. 211, Gongpinghuimin Road, Wenjiang District, Chengdu, 611130, Sichuan Province, People's Republic of China. 2. Adisseo SAS, Center of Excellence and Research in Nutrition, 03600, Malicorne, France. 3. Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Key laboratory of Animal Disease-resistant Nutrition and Feed of China Ministry of Agriculture and Rural Affairs, Key laboratory of Animal Disease-resistant Nutrition of Sichuan Province, Institute of Animal Nutrition, Sichuan Agricultural University, No. 211, Gongpinghuimin Road, Wenjiang District, Chengdu, 611130, Sichuan Province, People's Republic of China. acatmxb2003@163.com.
Abstract
BACKGROUND: Lentinan (LNT) may regulate many important physiological functions of human and animals. This study aimed to verify whether LNT administration could relieve diarrhea via improving gut immunity in rotavirus (RV)-challenged weaned pigs. METHODS: Twenty-eight weaned pigs were randomly fed 2 diets containing 0 or 84 mg/kg LNT product for 19 d (n = 14). RV infection was executed on d 15. After extracting polysaccharides from LNT product, its major monosaccharides were analyzed. Then, LNT polysaccharide was used to administrate RV-infected IPEC-J2 cells. RESULTS: Dietary LNT supplementation supported normal function of piglets even when infected with RV, as reflected by reduced growth performance loss and diarrhea prevalence, and maintained gut immunity (P < 0.05). The polysaccharide was isolated from LNT product, which molecular weight was 5303 Da, and major monosaccharides included glucose, arabinose and galactose. In RV-infected IPEC-J2 cells, this polysaccharide significantly increased cell viability (P < 0.05), and significantly increased anti-virus immunity via regulating pattern recognition receptors and host defense peptides (P < 0.05). CONCLUSION: Those results suggest that LNT administration increases the piglets' resistance to RV-induced stress, likely by supporting intestinal immunity.
BACKGROUND:Lentinan (LNT) may regulate many important physiological functions of human and animals. This study aimed to verify whetherLNT administration could relieve diarrhea via improving gut immunity in rotavirus (RV)-challenged weaned pigs. METHODS: Twenty-eight weaned pigs were randomly fed 2 diets containing 0 or 84 mg/kg LNT product for 19 d (n = 14). RV infection was executed on d 15. After extracting polysaccharides from LNT product, its major monosaccharides were analyzed. Then, LNT polysaccharide was used to administrate RV-infectedIPEC-J2 cells. RESULTS: Dietary LNT supplementation supported normal function of piglets even when infected with RV, as reflected by reduced growth performance loss and diarrhea prevalence, and maintained gut immunity (P < 0.05). The polysaccharide was isolated from LNT product, which molecular weight was 5303 Da, and major monosaccharides included glucose, arabinose and galactose. In RV-infectedIPEC-J2 cells, this polysaccharide significantly increased cell viability (P < 0.05), and significantly increased anti-virus immunity via regulating pattern recognition receptors and host defense peptides (P < 0.05). CONCLUSION: Those results suggest that LNT administration increases the piglets' resistance to RV-induced stress, likely by supporting intestinal immunity.
Entities:
Keywords:
Gut immunity; IPEC-J2 cells; Lentinan; Rotavirus; Weaned pigs