Zhimin Ji1, Wanjun Wu2, Fan Zhou3, Junfang Hu4, Qiuping Xu1, Weibin Yang3, Xueyong Peng1, Xinguo Wang5, Cheng Zhang6, Li Li7. 1. Department of Anesthesiology, Puren Hospital Affiliated to Wuhan University of Science and Technology, Wuhan, China. 2. Department of Anesthesiology, the Affiliated Suzhou Science and Technology Town Hospital of Nanjing Medical University, Suzhou, China. 3. Department of Clinical Laboratory, Puren Hospital, Affiliated to Wuhan University of Science and Technology, Wuhan, China. 4. Department of Pharmacy, Puren Hospital, Affiliated to Wuhan University of Science and Technology, Wuhan, China. 5. Wuhan Municipal Health Commission, Wuhan, China. 6. Department of Occupational Health, Wuhan Prevention and Treatment Center for Occupational Disease, Wuhan, China. 7. Department of Pathology, Puren Hospital Affiliated to Wuhan University of Science and Technology, Wuhan, China. lili1001@wust.edu.cn.
Abstract
BACKGROUND: Waste anesthetic gases (WAGs) leaked from new-type halogenated inhalational anesthetics such as sevoflurane have been were reported to pose a risk for the health of operating room personnel. The effects of WAGs on peripheral blood lymphocytes, however, remain yet controversial. The present study was undertaken to examine the effects of occupational sevoflurane exposure on the peripheral blood lymphocytes of medical personnel who work in the operating room. METHODS: A cohort of 56 medical residents were divided into exposed group (n = 28) and control group (non-exposed group) (n = 28). Gas chromatography was used to measure the concentration of sevoflurane in the medical resident's breathing zone during surgeries under inhalation anesthesia in the exposure group. The gas collection lasted an hour. Peripheral blood lymphocytes were isolated from venous blood, and then apoptosis and cell cycle were analyzed by flow cytometry. EDTA-anticoagulated whole blood was harvested to analyze the lymphocyte subsets by flow cytometry. Immunoglobulins (IgA, IgM, IgG) were quantified by immunoturbidimetry. RESULTS: The average concentration of sevoflurane in the exposed group was 1.03 ppm with a range from 0.03 ppm to 2.24 ppm. No significant effects were found on the apoptosis rates or cell cycles of peripheral blood lymphocytes in the exposed group relative to the control group (P > 0.05). Similarly, there were no significant differences in the lymphocyte subsets or the levels of immunoglobulins (IgA, IgM, IgG) between the two groups (P > 0.05). CONCLUSIONS: Occupational exposure to low-level sevoflurane has no significant effect on the peripheral blood lymphocytes of operating room staff, but this conclusion needs to be confirmed by multicenter and long-term follow-up studies with large samples. TRIAL REGISTRATION NUMBER AND DATE OF REGISTRATION: ChiCTR2000040772 , December 9, 2020 (Retrospective registration).
BACKGROUND: Waste anesthetic gases (WAGs) leaked from new-type halogenated inhalational anesthetics such as sevoflurane have been were reported to pose a risk for the health of operating room personnel. The effects of WAGs on peripheral blood lymphocytes, however, remain yet controversial. The present study was undertaken to examine the effects of occupational sevoflurane exposure on the peripheral blood lymphocytes of medical personnel who work in the operating room. METHODS: A cohort of 56 medical residents were divided into exposed group (n = 28) and control group (non-exposed group) (n = 28). Gas chromatography was used to measure the concentration of sevoflurane in the medical resident's breathing zone during surgeries under inhalation anesthesia in the exposure group. The gas collection lasted an hour. Peripheral blood lymphocytes were isolated from venous blood, and then apoptosis and cell cycle were analyzed by flow cytometry. EDTA-anticoagulated whole blood was harvested to analyze the lymphocyte subsets by flow cytometry. Immunoglobulins (IgA, IgM, IgG) were quantified by immunoturbidimetry. RESULTS: The average concentration of sevoflurane in the exposed group was 1.03 ppm with a range from 0.03 ppm to 2.24 ppm. No significant effects were found on the apoptosis rates or cell cycles of peripheral blood lymphocytes in the exposed group relative to the control group (P > 0.05). Similarly, there were no significant differences in the lymphocyte subsets or the levels of immunoglobulins (IgA, IgM, IgG) between the two groups (P > 0.05). CONCLUSIONS: Occupational exposure to low-level sevoflurane has no significant effect on the peripheral blood lymphocytes of operating room staff, but this conclusion needs to be confirmed by multicenter and long-term follow-up studies with large samples. TRIAL REGISTRATION NUMBER AND DATE OF REGISTRATION: ChiCTR2000040772 , December 9, 2020 (Retrospective registration).
Authors: Kátina M Souza; Leandro G Braz; Flávia R Nogueira; Marajane B Souza; Lahis F Bincoleto; Aline G Aun; José E Corrente; Lídia R Carvalho; José Reinaldo C Braz; Mariana G Braz Journal: Mutat Res Date: 2016-09-13 Impact factor: 2.433
Authors: Nils Kunze; Cathrin Weigel; Wolfgang Vautz; Katrin Schwerdtfeger; Melanie Jünger; Michael Quintel; Thorsten Perl Journal: J Occup Med Toxicol Date: 2015-03-27 Impact factor: 2.646
Authors: Joyce Lübbers; Marian H van Beers-Tas; Saskia Vosslamber; Samina A Turk; Sander de Ridder; Elise Mantel; John G Wesseling; Martine Reijm; Ingrid M van Hoogstraten; Johannes W Bijlsma; Dirkjan van Schaardenburg; Hetty J Bontkes; Cornelis L Verweij Journal: Arthritis Res Ther Date: 2016-09-14 Impact factor: 5.156
Authors: Lorena M C Lucio; Mariana G Braz; Paulo do Nascimento Junior; José Reinaldo C Braz; Leandro G Braz Journal: Braz J Anesthesiol Date: 2017-06-24