| Literature DB >> 33730513 |
Carolin Seyfferth1,2, Jim Renema1,2, Jos R Wendrich1,2, Thomas Eekhout1,2, Ruth Seurinck3,4, Niels Vandamme3,4, Bernhard Blob5,6, Yvan Saeys3,4, Yrjo Helariutta5,6, Kenneth D Birnbaum7, Bert De Rybel1,2.
Abstract
Single-cell approaches are quickly changing our view on biological systems by increasing the spatiotemporal resolution of our analyses to the level of the individual cell. The field of plant biology has fully embraced single-cell transcriptomics and is rapidly expanding the portfolio of available technologies and applications. In this review, we give an overview of the main advances in plant single-cell transcriptomics over the past few years and provide the reader with an accessible guideline covering all steps, from sample preparation to data analysis. We end by offering a glimpse of how these technologies will shape and accelerate plant-specific research in the near future.Entities:
Keywords: cell atlas; cell trajectory; plant transcriptomics; protoplast; scRNA-seq
Mesh:
Year: 2021 PMID: 33730513 PMCID: PMC7611048 DOI: 10.1146/annurev-arplant-081720-010120
Source DB: PubMed Journal: Annu Rev Plant Biol ISSN: 1543-5008 Impact factor: 26.379