Literature DB >> 33730354

Non-modified RNA-Based Reprogramming of Human Dermal Fibroblasts into Induced Pluripotent Stem Cells.

Immacolata Belviso1, Franca Di Meglio1, Veronica Romano1, Stefania Montagnani1, Clotilde Castaldo2.   

Abstract

The generation of pluripotent stem cells from adult somatic cells by cell reprogramming has put a whole new perspective on stem cell biology and stem cell-based regenerative medicine. Cell reprogramming acts through the introduction of key genes that regulate and maintain the pluripotent cell state. In this chapter, we describe the optimized protocol for the efficient isolation of fibroblasts from a skin punch biopsy and the subsequent easy and effective generation of integration-free induced pluripotent stem cell (iPSC) colonies forcing the expression of specific factors by non-modified RNAs.
© 2021. Springer Science+Business Media, LLC.

Entities:  

Keywords:  Cell Reprogramming; Dermal Fibroblasts; Induced Pluripotent Stem Cells (iPSCs); Integration-free; Non-modified RNAs (NM-RNAs); Regenerative Medicine

Mesh：

Substances：

Year:  2022        PMID： 33730354     DOI： 10.1007/7651_2021_364

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  15 in total

Review 1.  Ethical issues in stem cell research.

Authors:  Bernard Lo; Lindsay Parham
Journal:  Endocr Rev       Date:  2009-04-14       Impact factor: 19.871

Review 2.  Recent Advances in Therapeutic Applications of Induced Pluripotent Stem Cells.

Authors:  Farzaneh Rami; Shamsi Naderi Beni; Mahboobeh Mojaver Kahnamooi; Ilnaz Rahimmanesh; Ahmad Reza Salehi; Rasoul Salehi
Journal:  Cell Reprogram       Date:  2017-03-07       Impact factor: 1.987

Review 3.  An Insight into DNA-free Reprogramming Approaches to Generate Integration-free Induced Pluripotent Stem Cells for Prospective Biomedical Applications.

Authors:  Manash P Borgohain; Krishna Kumar Haridhasapavalan; Chandrima Dey; Poulomi Adhikari; Rajkumar P Thummer
Journal:  Stem Cell Rev Rep       Date:  2019-04       Impact factor: 5.739

4.  Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.

Authors:  Kazutoshi Takahashi; Shinya Yamanaka
Journal:  Cell       Date:  2006-08-10       Impact factor: 41.582

Review 5.  Induced pluripotent stem cells: how they will change the practice of cardiovascular medicine.

Authors:  Wing Tak Wong; Nazish Sayed; John P Cooke
Journal:  Methodist Debakey Cardiovasc J       Date:  2013 Oct-Dec

6.  Induction of pluripotent stem cells from adult human fibroblasts by defined factors.

Authors:  Kazutoshi Takahashi; Koji Tanabe; Mari Ohnuki; Megumi Narita; Tomoko Ichisaka; Kiichiro Tomoda; Shinya Yamanaka
Journal:  Cell       Date:  2007-11-30       Impact factor: 41.582

Review 7.  mRNA-Based Genetic Reprogramming.

Authors:  Luigi Warren; Cory Lin
Journal:  Mol Ther       Date:  2018-12-14       Impact factor: 11.454

Review 8.  Induced pluripotent stem cells: applications in regenerative medicine, disease modeling, and drug discovery.

Authors:  Vimal K Singh; Manisha Kalsan; Neeraj Kumar; Abhishek Saini; Ramesh Chandra
Journal:  Front Cell Dev Biol       Date:  2015-02-02

Review 9.  Advances in Pluripotent Stem Cells: History, Mechanisms, Technologies, and Applications.

Authors:  Gele Liu; Brian T David; Matthew Trawczynski; Richard G Fessler
Journal:  Stem Cell Rev Rep       Date:  2020-02       Impact factor: 5.739

Review 10.  iPSC technology--Powerful hand for disease modeling and therapeutic screen.

Authors:  Changsung Kim
Journal:  BMB Rep       Date:  2015-05       Impact factor: 4.778
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.