| Literature DB >> 33723266 |
Marie Luise Grünbein1, Alexander Gorel1, Lutz Foucar1, Sergio Carbajo2, William Colocho2, Sasha Gilevich2, Elisabeth Hartmann1, Mario Hilpert1, Mark Hunter2, Marco Kloos1,3, Jason E Koglin2,4, Thomas J Lane2,5, Jim Lewandowski2, Alberto Lutman2, Karol Nass1,6, Gabriela Nass Kovacs1, Christopher M Roome1, John Sheppard2, Robert L Shoeman1, Miriam Stricker1,7, Tim van Driel2, Sharon Vetter2, R Bruce Doak1, Sébastien Boutet2, Andrew Aquila2, Franz Josef Decker2, Thomas R M Barends1, Claudiu Andrei Stan8, Ilme Schlichting9.
Abstract
X-ray free-electron lasers (XFELs) enable obtaining novel insights in structural biology. The recently available MHz repetition rate XFELs allow full data sets to be collected in shorter time and can also decrease sample consumption. However, the microsecond spacing of MHz XFEL pulses raises new challenges, including possible sample damage induced by shock waves that are launched by preceding pulses in the sample-carrying jet. We explored this matter with an X-ray-pump/X-ray-probe experiment employing haemoglobin microcrystals transported via a liquid jet into the XFEL beam. Diffraction data were collected using a shock-wave-free single-pulse scheme as well as the dual-pulse pump-probe scheme. The latter, relative to the former, reveals significant degradation of crystal hit rate, diffraction resolution and data quality. Crystal structures extracted from the two data sets also differ. Since our pump-probe attributes were chosen to emulate EuXFEL operation at its 4.5 MHz maximum pulse rate, this prompts concern about such data collection.Entities:
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Year: 2021 PMID： 33723266 PMCID： PMC7960726 DOI： 10.1038/s41467-021-21819-8
Source DB: PubMed Journal: Nat Commun ISSN： 2041-1723 Impact factor: 14.919