| Literature DB >> 33718890 |
Jorge Domínguez-Andrés1,2, Rob J W Arts1,2, Siroon Bekkering1,2, Harsh Bahrar1,2, Bastiaan A Blok1,2, L Charlotte J de Bree1,2, Mariolina Bruno1,2, Özlem Bulut1,2, Priya A Debisarun1,2, Helga Dijkstra1,2, Jéssica Cristina Dos Santos1,2, Anaísa V Ferreira1,2, Daniela Flores-Gomez1,2, Laszlo A Groh1,2, Inge Grondman1,2, Leonie Helder1,2, Cor Jacobs1,2, Liesbeth Jacobs1,2, Trees Jansen1,2, Gizem Kilic1,2, Viola Klück1,2, Valerie A C M Koeken1,2,3, Heidi Lemmers1,2, Simone J C F M Moorlag1,2, Vera P Mourits1,2, Jelmer H van Puffelen1,2, Katrin Rabold1,2, Rutger J Röring1,2, Diletta Rosati1,2, Helin Tercan1,2, Julia van Tuijl1,2, Jessica Quintin4, Reinout van Crevel1,2, Niels P Riksen1,2, Leo A B Joosten1,2, Mihai G Netea1,2,5.
Abstract
A growing number of studies show that innate immune cells can undergo functional reprogramming, facilitating a faster and enhanced response to heterologous secondary stimuli. This concept has been termed "trained immunity." We outline here a protocol to recapitulate this in vitro using adherent monocytes from consecutive isolation of peripheral blood mononuclear cells. The induction of trained immunity and the associated functional reprogramming of monocytes is described in detail using β-glucan (from Candida albicans) and Bacillus Calmette-Guérin as examples. For complete details on the use and execution of this protocol, please refer to Repnik et al. (2003) and Bekkering et al. (2016).Entities:
Keywords: Cell biology; Cell isolation; Cell-based assays; Immunology
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Year: 2021 PMID： 33718890 PMCID： PMC7921712 DOI： 10.1016/j.xpro.2021.100365
Source DB: PubMed Journal: STAR Protoc ISSN： 2666-1667