Heba F Ibrahim1, Safinaz H Safwat2, Teshreen M Zeitoun2, Khaled F El Mulla3, Amira Y Medwar2. 1. Department of Histology and Cell Biology, Faculty of Medicine, Alexandria University, Dr Fahmi Abdelmeguid St., Al. Mowassat Campus, Alexandria, 21561, Egypt. drhebafathy@yahoo.com. 2. Department of Histology and Cell Biology, Faculty of Medicine, Alexandria University, Dr Fahmi Abdelmeguid St., Al. Mowassat Campus, Alexandria, 21561, Egypt. 3. Department of Dermatology, Venereology and Andrology, Faculty of Medicine, Alexandria University, Alexandria, 21514, Egypt.
Abstract
BACKGROUND: Busulfan is an alkylating chemotherapeutic agent that is routinely prescribed for leukemic patients to induce myelo-ablation. However, it also results in azoospermia and infertility in cancer survivors. This research was constructed to explore the possible therapeutic role of amniotic fluid-derived stem cells (AFSCs) in improving busulfan-induced azoospermia in adult rats. METHODS: Forty two adult male albino rats were randomized into: (1) control group, (2) azoospermia group, (3) spontaneous recovery group, and (4) AFSCs-treated group, in which AFSCs were transplanted through their injection into the testicular efferent ducts. The assessment included a histo-pathological examination of the seminiferous tubules by the light and transmission electron microscopes. Additionally, the confocal laser scanning microscope was used for confirmation of homing of the implanted cells. Moreover, we conducted an immuno-fluorescence study for detection of the proliferating cell nuclear antigen (PCNA) in the spermatogenic cells, epididymal sperm count, and a histo-morphometric study. RESULTS: AFSCs successfully homed over the basement membrane of the injured seminiferous tubules. They greatly attenuated busulfan-induced degenerative and oxidative changes. They also caused a re-expression of PCNA in the germ cells, leading to resumption of spermatogenesis and re-appearance of spermatozoa. CONCLUSION: AFSCs could be a promising treatment modality for male infertility induced by chemotherapy, as they possess prominent regenerative, anti-apoptotic, and anti-inflammatory potentials.
BACKGROUND: Busulfan is an alkylating chemotherapeutic agent that is routinely prescribed for leukemic patients to induce myelo-ablation. However, it also results in azoospermia and infertility in cancer survivors. This research was constructed to explore the possible therapeutic role of amniotic fluid-derived stem cells (AFSCs) in improving busulfan-induced azoospermia in adult rats. METHODS: Forty two adult male albino rats were randomized into: (1) control group, (2) azoospermia group, (3) spontaneous recovery group, and (4) AFSCs-treated group, in which AFSCs were transplanted through their injection into the testicular efferent ducts. The assessment included a histo-pathological examination of the seminiferous tubules by the light and transmission electron microscopes. Additionally, the confocal laser scanning microscope was used for confirmation of homing of the implanted cells. Moreover, we conducted an immuno-fluorescence study for detection of the proliferating cell nuclear antigen (PCNA) in the spermatogenic cells, epididymal sperm count, and a histo-morphometric study. RESULTS: AFSCs successfully homed over the basement membrane of the injured seminiferous tubules. They greatly attenuated busulfan-induced degenerative and oxidative changes. They also caused a re-expression of PCNA in the germ cells, leading to resumption of spermatogenesis and re-appearance of spermatozoa. CONCLUSION: AFSCs could be a promising treatment modality for male infertility induced by chemotherapy, as they possess prominent regenerative, anti-apoptotic, and anti-inflammatory potentials.
Authors: A Pantalone; I Antonucci; M Guelfi; P Pantalone; F G Usuelli; L Stuppia; V Salini Journal: Eur Rev Med Pharmacol Sci Date: 2016-07 Impact factor: 3.507
Authors: Imke H Bartelink; Arief Lalmohamed; Elisabeth M L van Reij; Christopher C Dvorak; Rada M Savic; Juliette Zwaveling; Robbert G M Bredius; Antoine C G Egberts; Marc Bierings; Morris Kletzel; Peter J Shaw; Christa E Nath; George Hempel; Marc Ansari; Maja Krajinovic; Yves Théorêt; Michel Duval; Ron J Keizer; Henrique Bittencourt; Moustapha Hassan; Tayfun Güngör; Robert F Wynn; Paul Veys; Geoff D E Cuvelier; Sarah Marktel; Robert Chiesa; Morton J Cowan; Mary A Slatter; Melisa K Stricherz; Cathryn Jennissen; Janel R Long-Boyle; Jaap Jan Boelens Journal: Lancet Haematol Date: 2016-10-13 Impact factor: 18.959