Literature DB >> 337130

Transformation of Escherichia coli by a specific DNA restriction fragment.

S M Schweitzer, H Matzura.   

Abstract

Specific transformation of a rifampicin sensitive strain of Escherichia coli to rifampicin resistance has been performed by a single, defined DNA restriction fragment carrying the genetic information for the beta subunit of E. coli RNA polymerase. In this transformation the transforming genetic character has been substituted for the corresponding recipient gene locus by recombination. The value of the described transformation system for locating genetic markers on DNA restriction fragments is discussed in comparison to previously reported in vitro systems.

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Year:  1977        PMID： 337130     DOI： 10.1007/bf00393162

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  19 in total

1.  Reconstitution of bacterial DNA-dependent RNA-polymerase from isolated subunits as a tool for the elucidation of the role of the subunits in transcription.

Authors:  A Heil; W Zillig
Journal:  FEBS Lett       Date:  1970-12       Impact factor: 4.124

2.  Evidence for a lambda transducing phage carrying the genes for the beta and beta' subunits of Escherichia coli RNA polymerase.

Authors:  J B Kirschbaum; J Scaife
Journal:  Mol Gen Genet       Date:  1974

3.  Construction of biologically functional bacterial plasmids in vitro.

Authors:  S N Cohen; A C Chang; H W Boyer; R B Helling
Journal:  Proc Natl Acad Sci U S A       Date:  1973-11       Impact factor: 11.205

4.  Specialised transformation in Escherichia coli K12.

Authors:  M Oishi; S D Cosloy
Journal:  Nature       Date:  1974-03-08       Impact factor: 49.962

5.  The genetic and biochemical basis of the transformability of Escherichia coli K12.

Authors:  M Oishi; S D Cosloy
Journal:  Biochem Biophys Res Commun       Date:  1972-12-18       Impact factor: 3.575

6.  Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.

Authors:  D B Clewell
Journal:  J Bacteriol       Date:  1972-05       Impact factor: 3.490

7.  A new method for the large scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase.

Authors:  R R Burgess
Journal:  J Biol Chem       Date:  1969-11-25       Impact factor: 5.157

8.  Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.

Authors:  D B Clewell; D R Helinski
Journal:  Proc Natl Acad Sci U S A       Date:  1969-04       Impact factor: 11.205

9.  DNA blockade by rifampicin-inactivated Escherichia coli RNA polymerase, and its amelioration by a specific mutation.

Authors: 
Journal:  Eur J Biochem       Date:  1976-12

10.  Genetic transformation in Escherichia coli K12.

Authors:  S D Cosloy; M Oishi
Journal:  Proc Natl Acad Sci U S A       Date:  1973-01       Impact factor: 11.205
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  4 in total

1.  Control features within the rplJL-rpoBC transcription unit of Escherichia coli.

Authors:  G Barry; C L Squires; C Squires
Journal:  Proc Natl Acad Sci U S A       Date:  1979-10       Impact factor: 11.205

2.  Contranscription of genes for RNA polymerase subunits beta and beta' with genes for ribosomal proteins in Escherichia coli.

Authors:  M Yamamoto; M Nomura
Journal:  Proc Natl Acad Sci U S A       Date:  1978-08       Impact factor: 11.205

3.  Evidence for co-transcription of the RNA polymerase genes rpoBC with a ribosomal protein gene of escherichia coli.

Authors:  A J Newman; T G Linn; R S Hayward
Journal:  Mol Gen Genet       Date:  1979-01-31

4.  Cloning of DNA of the rpoBC operon from the chromosome of Escherichia coli K12.

Authors:  A Newman; R S Hayward
Journal:  Mol Gen Genet       Date:  1980-02
  4 in total

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