The polyploidizing growth pattern of normal rat liver is replaced by divisional, diploid growth in hepatocellular nodules and carcinomas.

DNA content was measured by flow cytometry in isolated nuclei from 71 neoplastic nodules and 15 hepatocellular carcinomas isolated from rat liver at various times after treatment with an initiation--promotion regimen employing diethylnitrosamine and 2-acetylaminofluorene. Nodules and carcinomas contained mostly diploid nuclei as compared with both surrounding and normal hepatocytes which were predominantly polyploid. There appears to be a positive correlation between the degree of diploidy in nodules and their rate of proliferation. No aneuploid populations were identified in any neoplasm despite good peak resolution. These results show that an alteration in proliferation pattern from normal polyploidizing growth to diploid--diploid divisional growth is a consistent characteristic throughout the carcinogenic process in our experimental model.