| Literature DB >> 33704708 |
Abstract
Glutamine is a major substrate for biosynthesis. It contributes to multiple pathways required for cell proliferation, supports antioxidant defense via glutathione synthesis, and sustains the tricarboxylic acid (TCA) cycle through anaplerosis. Glutamine-fueled anaplerosis and related biosynthesis can be studied in detail in melanoma using stable isotope (13C) labeling followed by gas chromatography-mass spectrometry (GC-MS) analysis of metabolite amounts and labeling. Detailed protocols for the assay of polar metabolites (including amino acids, TCA cycle, and glycolysis metabolites) and fatty acids by these methods following cell treatment with 13C-glutamine or 13C-glucose are presented.Entities:
Keywords: Amino acids; Fatty acids; GC-MS; Glutamine; Glycolysis; Melanoma; Metabolite quantification; Stable-isotope tracing; Tricarboxylic acid cycle
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Year: 2021 PMID： 33704708 DOI： 10.1007/978-1-0716-1205-7_7
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745