| Literature DB >> 33704705 |
Juliano T Freitas1, Ivan Jozic2, Barbara Bedogni2.
Abstract
Cell migration is a critical process involved in morphogenesis, inflammation, and cancer metastasis. Wound healing assay is a simple, non-expensive, and highly reproducible method to study cancer cell migration in vitro. It is based on the observation that cells growing in a monolayer migrate to re-establish cell contacts after the development of an artificial wound. The assay involves creation of a wound in a monolayer, image acquisition during wound closure, and comparison of migrated area at initial and final time points.Entities:
Keywords: Cell migration; Melanoma; Metastasis; Wound healing; Wound scratch
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Year: 2021 PMID: 33704705 DOI: 10.1007/978-1-0716-1205-7_4
Source DB: PubMed Journal: Methods Mol Biol ISSN: 1064-3745