| Literature DB >> 33689075 |
G N Singina1, P V Sergiev2,3,4, A V Lopukhov5, M P Rubtsova4, N P Taradajnic5, N V Ravin6, E N Shedova5, T E Taradajnic5, I A Polejaeva7, A V Dozev5, G Brem8, O A Dontsova3,4,9,10, N A Zinovieva5.
Abstract
Somatic Cell Nuclear Transfer (SCNT) technique was used to produce the first viable cloned cattle offspring in Russia. Whole-genome SNP genotyping confirmed that the cloned calf was identical to the fibroblast cell line that was used for SCNT. CRISPR/Cas9 approach was subsequently used to knock out genes for beta-lactoglobulin gene (PAEP) and the beta-lactoglobulin-like protein gene (LOC100848610) in the fibroblast cells. Gene editing (GE) efficiency was 4.4% for each of these genes. We successfully obtained single-cell-derived fibroblast colonies containing PAEP and LOC100848610 knockouts, which will be used to produce beta-lactoglobulin-deficient cattle.Entities:
Keywords: Bos taurus; beta-lactoglobulin knock-out; gene editing; somatic cloning
Mesh：
Year: 2021 PMID： 33689075 PMCID： PMC7946654 DOI： 10.1134/S1607672921010099
Source DB: PubMed Journal: Dokl Biochem Biophys ISSN： 1607-6729 Impact factor: 0.788