| Literature DB >> 16716426 |
Daniel Salamone1, Lino Barañao, Claudio Santos, Leonardo Bussmann, Jorge Artuso, Carlos Werning, Aida Prync, Cesar Carbonetto, Susana Dabsys, Carlos Munar, Roberto Salaberry, Guillermo Berra, Ignacio Berra, Nahuel Fernández, Mariana Papouchado, Marcelo Foti, Norberto Judewicz, Ignacio Mujica, Luciana Muñoz, Silvina Fenández Alvarez, Eliseo González, Juan Zimmermann, Marcelo Criscuolo, Carlos Melo.
Abstract
Transgenic farm animals have been proposed as an alternative to current bioreactors for large scale production of biopharmaceuticals. However, the efficiency of both methods in the production of the same protein has not yet been established. Here we report the production of recombinant human growth hormone (hGH) in the milk of a cloned transgenic cow at levels of up to 5 g l(-1). The hormone is identical to that currently produced by expression in E. coli. In addition, the hematological and somatometric parameters of the cloned transgenic cow are within the normal range for the breed and it is fertile and capable of producing normal offspring. These results demonstrate that transgenic cattle can be used as a cost-effective alternative for the production of this hormone.Entities:
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Year: 2006 PMID： 16716426 DOI： 10.1016/j.jbiotec.2006.01.005
Source DB: PubMed Journal: J Biotechnol ISSN： 0168-1656 Impact factor: 3.307