Literature DB >> 33660218

The highly flexible disordered regions of the SARS-CoV-2 nucleocapsid N protein within the 1-248 residue construct: sequence-specific resonance assignments through NMR.

Marco Schiavina1,2, Letizia Pontoriero1,2, Vladimir N Uversky3, Isabella C Felli4,5, Roberta Pierattelli6,7.   

Abstract

The nucleocapsid protein N from SARS-CoV-2 is one of the most highly expressed proteins by the virus and plays a number of important roles in the transcription and assembly of the virion within the infected host cell. It is expected to be characterized by a highly dynamic and heterogeneous structure as can be inferred by bioinformatics analyses as well as from the data available for the homologous protein from SARS-CoV. The two globular domains of the protein (NTD and CTD) have been investigated while no high-resolution information is available yet for the flexible regions of the protein. We focus here on the 1-248 construct which comprises two disordered fragments (IDR1 and IDR2) in addition to the N-terminal globular domain (NTD) and report the sequence-specific assignment of the two disordered regions, a step forward towards the complete characterization of the whole protein.

Entities:  

Keywords:  13C detection; Covid-19; IDPs; NMR spectroscopy; Nucleocapsid protein; SARS-CoV-2

Mesh：

Substances：

Year:  2021        PMID： 33660218      PMCID： PMC7928198          DOI： 10.1007/s12104-021-10009-8

Source DB:  PubMed          Journal:  Biomol NMR Assign        ISSN： 1874-270X            Impact factor:   0.746


  14 in total

1.  Plasticity in structure and assembly of SARS-CoV-2 nucleocapsid protein.

Authors:  Huaying Zhao; Ai Nguyen; Di Wu; Yan Li; Sergio A Hassan; Jiji Chen; Hari Shroff; Grzegorz Piszczek; Peter Schuck
Journal:  PNAS Nexus       Date:  2022-05-16

2.  Atomic-Resolution Structure of SARS-CoV-2 Nucleocapsid Protein N-Terminal Domain.

Authors:  Sucharita Sarkar; Brent Runge; Ryan W Russell; Kumar Tekwani Movellan; Daniel Calero; Somayeh Zeinalilathori; Caitlin M Quinn; Manman Lu; Guillermo Calero; Angela M Gronenborn; Tatyana Polenova
Journal:  J Am Chem Soc       Date:  2022-05-31       Impact factor: 16.383

Review 3.  13C Direct Detected NMR for Challenging Systems.

Authors:  Isabella C Felli; Roberta Pierattelli
Journal:  Chem Rev       Date:  2022-01-13       Impact factor: 72.087

4.  SARS-CoV-2 nucleocapsid protein interacts with immunoregulators and stress granules and phase separates to form liquid droplets.

Authors:  Syam Prakash Somasekharan; Martin Gleave
Journal:  FEBS Lett       Date:  2021-11-22       Impact factor: 3.864

5.  Plasticity in structure and assembly of SARS-CoV-2 nucleocapsid protein.

Authors:  Huaying Zhao; Ai Nguyen; Di Wu; Yan Li; Sergio A Hassan; Jiji Chen; Hari Shroff; Grzegorz Piszczek; Peter Schuck
Journal:  bioRxiv       Date:  2022-02-09

6.  Intrinsically Disordered Proteins: Perspective on COVID-19 Infection and Drug Discovery.

Authors:  Rumiana Tenchov; Qiongqiong Angela Zhou
Journal:  ACS Infect Dis       Date:  2022-02-23       Impact factor: 5.578

Review 7.  The SARS-CoV-2 Nucleocapsid Protein and Its Role in Viral Structure, Biological Functions, and a Potential Target for Drug or Vaccine Mitigation.

Authors:  Zhihua Bai; Ying Cao; Wenjun Liu; Jing Li
Journal:  Viruses       Date:  2021-06-10       Impact factor: 5.048

8.  Large-Scale Recombinant Production of the SARS-CoV-2 Proteome for High-Throughput and Structural Biology Applications.

Authors:  Nadide Altincekic; Sophie Marianne Korn; Nusrat Shahin Qureshi; Marie Dujardin; Martí Ninot-Pedrosa; Rupert Abele; Marie Jose Abi Saad; Caterina Alfano; Fabio C L Almeida; Islam Alshamleh; Gisele Cardoso de Amorim; Thomas K Anderson; Cristiane D Anobom; Chelsea Anorma; Jasleen Kaur Bains; Adriaan Bax; Martin Blackledge; Julius Blechar; Anja Böckmann; Louis Brigandat; Anna Bula; Matthias Bütikofer; Aldo R Camacho-Zarco; Teresa Carlomagno; Icaro Putinhon Caruso; Betül Ceylan; Apirat Chaikuad; Feixia Chu; Laura Cole; Marquise G Crosby; Vanessa de Jesus; Karthikeyan Dhamotharan; Isabella C Felli; Jan Ferner; Yanick Fleischmann; Marie-Laure Fogeron; Nikolaos K Fourkiotis; Christin Fuks; Boris Fürtig; Angelo Gallo; Santosh L Gande; Juan Atilio Gerez; Dhiman Ghosh; Francisco Gomes-Neto; Oksana Gorbatyuk; Serafima Guseva; Carolin Hacker; Sabine Häfner; Bing Hao; Bruno Hargittay; K Henzler-Wildman; Jeffrey C Hoch; Katharina F Hohmann; Marie T Hutchison; Kristaps Jaudzems; Katarina Jović; Janina Kaderli; Gints Kalniņš; Iveta Kaņepe; Robert N Kirchdoerfer; John Kirkpatrick; Stefan Knapp; Robin Krishnathas; Felicitas Kutz; Susanne Zur Lage; Roderick Lambertz; Andras Lang; Douglas Laurents; Lauriane Lecoq; Verena Linhard; Frank Löhr; Anas Malki; Luiza Mamigonian Bessa; Rachel W Martin; Tobias Matzel; Damien Maurin; Seth W McNutt; Nathane Cunha Mebus-Antunes; Beat H Meier; Nathalie Meiser; Miguel Mompeán; Elisa Monaca; Roland Montserret; Laura Mariño Perez; Celine Moser; Claudia Muhle-Goll; Thais Cristtina Neves-Martins; Xiamonin Ni; Brenna Norton-Baker; Roberta Pierattelli; Letizia Pontoriero; Yulia Pustovalova; Oliver Ohlenschläger; Julien Orts; Andrea T Da Poian; Dennis J Pyper; Christian Richter; Roland Riek; Chad M Rienstra; Angus Robertson; Anderson S Pinheiro; Raffaele Sabbatella; Nicola Salvi; Krishna Saxena; Linda Schulte; Marco Schiavina; Harald Schwalbe; Mara Silber; Marcius da Silva Almeida; Marc A Sprague-Piercy; Georgios A Spyroulias; Sridhar Sreeramulu; Jan-Niklas Tants; Kaspars Tārs; Felix Torres; Sabrina Töws; Miguel Á Treviño; Sven Trucks; Aikaterini C Tsika; Krisztina Varga; Ying Wang; Marco E Weber; Julia E Weigand; Christoph Wiedemann; Julia Wirmer-Bartoschek; Maria Alexandra Wirtz Martin; Johannes Zehnder; Martin Hengesbach; Andreas Schlundt
Journal:  Front Mol Biosci       Date:  2021-05-10

9.  The inherent dynamics and interaction sites of the SARS-CoV-2 nucleocapsid N-terminal region.

Authors:  Jasmina S Redzic; Eunjeong Lee; Alexandra Born; Aaron Issaian; Morkos A Henen; Parker Nichols; Ashley Blue; Kirk C Hansen; Angelo D'Alessandro; Beat Vögeli; Elan Zohar Eisenmesser
Journal:  J Mol Biol       Date:  2021-06-20       Impact factor: 5.469

10.  The intrinsically disordered SARS-CoV-2 nucleoprotein in dynamic complex with its viral partner nsp3a.

Authors:  Luiza Mamigonian Bessa; Serafima Guseva; Aldo R Camacho-Zarco; Nicola Salvi; Damien Maurin; Laura Mariño Perez; Maiia Botova; Anas Malki; Max Nanao; Malene Ringkjøbing Jensen; Rob W H Ruigrok; Martin Blackledge
Journal:  Sci Adv       Date:  2022-01-19       Impact factor: 14.136
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