Literature DB >> 33659556

Circadian Gene Profiling in Laser Capture Microdissected Mouse Club Cells.

Zhenguang Zhang1, Andrew Loudon1.   

Abstract

Cell heterogeneity is high in tissues like lung. Research conducted on pure population of cells usually offers more insights than bulk tissues, such as circadian clock work. In this protocol, we provide a detailed work flow on how to do circadian clock study by RNA seq in laser capture micro-dissected mouse lung club cells. The method uses frozen tissues and is highly reproduciable.
Copyright © 2020 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Circadian clock; Club cell; Laser capture micro-dissection; Lung; RNA seq

Year:  2020        PMID： 33659556      PMCID： PMC7842306          DOI： 10.21769/BioProtoc.3590

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  8 in total

1.  Laser capture microdissection and mRNA characterization of mouse airway epithelium: methodological considerations.

Authors:  Tomoko Betsuyaku; Robert M Senior
Journal:  Micron       Date:  2004       Impact factor: 2.251

2.  Considerations for RNA-seq analysis of circadian rhythms.

Authors:  Jiajia Li; Gregory R Grant; John B Hogenesch; Michael E Hughes
Journal:  Methods Enzymol       Date:  2014-12-26       Impact factor: 1.600

3.  A circadian gene expression atlas in mammals: implications for biology and medicine.

Authors:  Ray Zhang; Nicholas F Lahens; Heather I Ballance; Michael E Hughes; John B Hogenesch
Journal:  Proc Natl Acad Sci U S A       Date:  2014-10-27       Impact factor: 11.205

4.  A robust RNA integrity-preserving staining protocol for laser capture microdissection of endometrial cancer tissue.

Authors:  Michele Cummings; Ciara V McGinley; Nafisa Wilkinson; Sarah L Field; Sean R Duffy; Nicolas M Orsi
Journal:  Anal Biochem       Date:  2011-05-12       Impact factor: 3.365

5.  Light-dark oscillations in the lung transcriptome: implications for lung homeostasis, repair, metabolism, disease, and drug action.

Authors:  Siddharth Sukumaran; William J Jusko; Debra C Dubois; Richard R Almon
Journal:  J Appl Physiol (1985)       Date:  2011-03-24

6.  An epithelial circadian clock controls pulmonary inflammation and glucocorticoid action.

Authors:  Julie Gibbs; Louise Ince; Laura Matthews; Junjie Mei; Thomas Bell; Nan Yang; Ben Saer; Nicola Begley; Toryn Poolman; Marie Pariollaud; Stuart Farrow; Francesco DeMayo; Tracy Hussell; G Scott Worthen; David Ray; Andrew Loudon
Journal:  Nat Med       Date:  2014-07-27       Impact factor: 53.440

7.  Circadian timing in the lung; a specific role for bronchiolar epithelial cells.

Authors:  J E Gibbs; S Beesley; J Plumb; D Singh; S Farrow; D W Ray; A S I Loudon
Journal:  Endocrinology       Date:  2008-09-11       Impact factor: 4.736

8.  Circadian rhythm reprogramming during lung inflammation.

Authors:  Jeffrey A Haspel; Sukrutha Chettimada; Rahamthulla S Shaik; Jen-Hwa Chu; Benjamin A Raby; Manuela Cernadas; Vincent Carey; Vanessa Process; G Matthew Hunninghake; Emeka Ifedigbo; James A Lederer; Joshua Englert; Ashley Pelton; Anna Coronata; Laura E Fredenburgh; Augustine M K Choi
Journal:  Nat Commun       Date:  2014-09-11       Impact factor: 14.919
  8 in total

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