Literature DB >> 33659351

RNA Stability Measurements Using RT-qPCR in Arabidopsis Seedlings.

Tianran Jia1, Brandon H Le2.   

Abstract

Steady-state mRNA levels are determined by both the rates of transcription and degradation. Regulation of mRNA stability and/or degradation are key factors that can significantly affect mRNA levels and its biological functions. mRNA stability can be measured indirectly after transcription inhibition. This protocol described a rapid and sensitive method of mRNA stability measurement through quantitative reverse transcription PCR (RT-qPCR) after inhibition of RNA transcription by cordycepin in Arabidopsis seedlings.
Copyright © 2020 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Arabidopsis; Cordycepin; Primary MicroRNA Transcripts; RNA Degradation; RNA Stability; RT-qPCR

Year:  2020        PMID： 33659351      PMCID： PMC7842415          DOI： 10.21769/BioProtoc.3680

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  6 in total

1.  Analyzing real-time PCR data by the comparative C(T) method.

Authors:  Thomas D Schmittgen; Kenneth J Livak
Journal:  Nat Protoc       Date:  2008       Impact factor: 13.491

2.  The Arabidopsis MOS4-Associated Complex Promotes MicroRNA Biogenesis and Precursor Messenger RNA Splicing.

Authors:  Tianran Jia; Bailong Zhang; Chenjiang You; Yong Zhang; Liping Zeng; Shengjun Li; Kaeli C M Johnson; Bin Yu; Xin Li; Xuemei Chen
Journal:  Plant Cell       Date:  2017-09-25       Impact factor: 11.277

3.  Handling Arabidopsis plants: growth, preservation of seeds, transformation, and genetic crosses.

Authors:  Luz Rivero; Randy Scholl; Nicholas Holomuzki; Deborah Crist; Erich Grotewold; Jelena Brkljacic
Journal:  Methods Mol Biol       Date:  2014

4.  Circadian control of messenger RNA stability. Association with a sequence-specific messenger RNA decay pathway.

Authors:  Preetmoninder Lidder; Rodrigo A Gutiérrez; Patrice A Salomé; C Robertson McClung; Pamela J Green
Journal:  Plant Physiol       Date:  2005-07-29       Impact factor: 8.340

5.  Primer3--new capabilities and interfaces.

Authors:  Andreas Untergasser; Ioana Cutcutache; Triinu Koressaar; Jian Ye; Brant C Faircloth; Maido Remm; Steven G Rozen
Journal:  Nucleic Acids Res       Date:  2012-06-22       Impact factor: 16.971

6.  PRL1, an RNA-binding protein, positively regulates the accumulation of miRNAs and siRNAs in Arabidopsis.

Authors:  Shuxin Zhang; Yuhui Liu; Bin Yu
Journal:  PLoS Genet       Date:  2014-12-04       Impact factor: 5.917
  6 in total
  1 in total

1.  Arabidopsis AAR2, a conserved splicing factor in eukaryotes, acts in microRNA biogenesis.

Authors:  Lusheng Fan; Bin Gao; Ye Xu; Nora Flynn; Brandon Le; Chenjiang You; Shaofang Li; Natalia Achkar; Pablo A Manavella; Zhenbiao Yang; Xuemei Chen
Journal:  Proc Natl Acad Sci U S A       Date:  2022-10-03       Impact factor: 12.779
  1 in total

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