Literature DB >> 33654916

Differentiation of Mouse Embryonic Stem Cells to Neuronal Cells Using Hanging Droplets and Retinoic Acid.

Jeroen Witteveldt1, Sara Macias1.   

Abstract

Controlled differentiation of embryonic stem cells is an essential tool in stem cell research. In this protocol, we describe a simple differentiation protocol involving the induction of embryoid body formation in mouse embryonic stem cells (mESC) using hanging droplets, followed by differentiation into a neuronal lineage.
Copyright © 2019 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Differentiation; Embryoid bodies; Embryonic stem cells; Hanging droplets; Neuronal; Retinoic acid

Year:  2019        PMID： 33654916      PMCID： PMC7853965          DOI： 10.21769/BioProtoc.3417

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  8 in total

1.  Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture.

Authors:  Qi-Long Ying; Marios Stavridis; Dean Griffiths; Meng Li; Austin Smith
Journal:  Nat Biotechnol       Date:  2003-01-13       Impact factor: 54.908

2.  Purification of embryonic stem cell-derived neurons by immunoisolation.

Authors:  Kay Jüngling; Karl Nägler; Frank W Pfrieger; Kurt Gottmann
Journal:  FASEB J       Date:  2003-09-18       Impact factor: 5.191

3.  Pluripotent mouse embryonic stem cells are able to differentiate into cardiomyocytes expressing chronotropic responses to adrenergic and cholinergic agents and Ca2+ channel blockers.

Authors:  A M Wobus; G Wallukat; J Hescheler
Journal:  Differentiation       Date:  1991-12       Impact factor: 3.880

4.  Lineage selection of functional and cryopreservable human embryonic stem cell-derived neurons.

Authors:  Julia Ladewig; Philipp Koch; Elmar Endl; Banu Meiners; Thoralf Opitz; Sebastien Couillard-Despres; Ludwig Aigner; Oliver Brüstle
Journal:  Stem Cells       Date:  2008-04-17       Impact factor: 6.277

5.  Differentiation of human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers.

Authors:  J Itskovitz-Eldor; M Schuldiner; D Karsenti; A Eden; O Yanuka; M Amit; H Soreq; N Benvenisty
Journal:  Mol Med       Date:  2000-02       Impact factor: 6.354

6.  Retinoic acid-induced neural differentiation of embryonal carcinoma cells.

Authors:  E M Jones-Villeneuve; M A Rudnicki; J F Harris; M W McBurney
Journal:  Mol Cell Biol       Date:  1983-12       Impact factor: 4.272

7.  MicroRNA-deficient mouse embryonic stem cells acquire a functional interferon response.

Authors:  Jeroen Witteveldt; Lisanne I Knol; Sara Macias
Journal:  Elife       Date:  2019-04-23       Impact factor: 8.140

8.  Neural differentiation of embryonic stem cells in vitro: a road map to neurogenesis in the embryo.

Authors:  Elsa Abranches; Margarida Silva; Laurent Pradier; Herbert Schulz; Oliver Hummel; Domingos Henrique; Evguenia Bekman
Journal:  PLoS One       Date:  2009-07-21       Impact factor: 3.240
  8 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.