| Literature DB >> 33654789 |
Francesca Pischedda1, Giovanni Piccoli1.
Abstract
Primary neuronal culture from rodents is a key tool in neurobiology. However, the preparation of primary cultures requires precise planning, starting from animal mating. Furthermore, each preparation generates a high amount of cells that eventually go wasted. The possibility to cryopreserve primary neural cells represents a resource for in vitro studies and significantly reduces the sacrifice of animals. Here we describe that Neurostore buffer supports the cryopreservation of primary neurons.Entities:
Keywords: Cryopreservation; Long-term storage; Neurons; Reproducibility; Specimen sharing
Year: 2019 PMID: 33654789 PMCID: PMC7854206 DOI: 10.21769/BioProtoc.3270
Source DB: PubMed Journal: Bio Protoc ISSN: 2331-8325