| Literature DB >> 19895801 |
Xue-Hu Ma1, Ying Shi, Ying Hou, Yang Liu, Lei Zhang, Wen-Xia Fan, Dan Ge, Tian-Qing Liu, Zhan-Feng Cui.
Abstract
Neural stem cells (NSCs) are of great value for clinical application and scientific research. The development of efficient cryopreservation protocols could significantly facilitate the storage and transportation for clinic applications. The objective of the present study is to improve the survival rate and viability of NSCs. Neural stem cells with three states of single-cell suspension, NSC spheres with diameters of 30-50 microm and 80-100 microm, were cryopreserved by slow-freezing method with the cryoprotective agent (CPA) of dimethyl sulfoxide (Me(2)SO), respectively. Then the post-thawing NSCs were tested for the survival rate and the differentiation ability. As a result, NSC spheres with diameter of 80-100 microm and Me(2)SO concentration of 8% achieve the survival rate of 82.9%, and the NSCs still sustain the multi-differentiation potentiality. These results indicated that both the subtle interaction among NSCs and sphere diameters may affect the survival rate together. Copyright 2009 Elsevier Inc. All rights reserved.Entities:
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Year: 2009 PMID： 19895801 DOI： 10.1016/j.cryobiol.2009.10.013
Source DB: PubMed Journal: Cryobiology ISSN： 0011-2240 Impact factor: 2.487