Kicheon Park1, Anuki C Liyanage1, Alan P Koretsky2, Yingtian Pan1, Congwu Du1. 1. Department of Biomedical Engineering, Stony Brook University, Stony Brook, NY, USA. 2. Laboratory of Functional and Molecular Imaging, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA.
Abstract
BACKGROUND: Genetically encoded calcium indicators (GECIs), especially the GCaMP-based green fluorescence GECIs have been widely used for in vivo detection of neuronal activity in rodents by measuring intracellular neuronal Ca2+ changes. More recently, jRGECO1a, a red shifted GECI, has been reported to detect neuronal Ca2+ activation. This opens the possibility of using dual-color GECIs for simultaneous interrogation of different cell populations. However, there has been no report to compare the functional difference between these two GECIs for in vivo imaging. Here, a comparative study is reported on neuronal responses to sensory stimulation using GCaMP6f and jRGECO1a that were virally delivered into the neurons in the somatosensory cortex of two different groups of animals, respectively. METHODS: GCaMP6f and jRGECO1a GECI were virally delivered to sensory cortex. After 3-4 weeks, the animals were imaged to capture the spatiotemporal changes of neuronal Ca2+ and the hemodynamic responses to forepaw electrical stimulation (0.3 mA, 0.3 ms/pulse, 0.03 Hz). The stimulation-evoked neuronal Ca2+ transients expressed with GCaMP6f or jRGECO1a were recorded during the baseline period and after an acute cocaine administration (1 mg/kg, i.v.). RESULTS: Histology confirmed that the efficiency of jRGECO1a and GCaMP6f expression into the cortical neurons was similar, i.e., 34%±3% and 32.7%±1.6%, respectively. Our imaging in vivo showed that the hemodynamic responses to the stimulation were the same between jRGECO1a and GCaMP6f expressed groups. Although the stimulation-evoked fluorescence change (∆F/F) and the time-to-peak of the neuronal Ca2+ transients were not significantly different between these two indicators, the full-width-half-maximum (FWHM) duration of the ∆F/F rise in the jRGECO1a-expressed group (0.16±0.02 s) was ~50 ms or 46% longer than that of the GCaMP6f group (0.11±0.003 s), indicating a longer recovery time in jRGECO1a than in GCaMP6f transients (P<0.01). This is likely due to the longer off rate of jRGECO1a than that of GCaMP6f. After cocaine, the time-to-peak of Ca2+ transients was delayed and their FWHM duration was prolonged for both expression groups, indicating that these are cocaine's effects on neuronal Ca2+ signaling and not artifacts due to the property differences of the GCEIs. CONCLUSIONS: This study shows that both jRGECO1a and GCaMP6f have sufficient sensitivity for tracking single-stimulation-evoked Ca2+ transients to detect neuronal activities from the brain. Since these GECIs are emitted at the different wavelengths, it will be possible to use them together to characterize the activity of different cell types (e.g., neurons and astrocytes) to study brain activation and brain functional changes in normal or diseased brains. 2021 Quantitative Imaging in Medicine and Surgery. All rights reserved.
BACKGROUND: Genetically encoded calcium indicators (GECIs), especially the GCaMP-based green fluorescence GECIs have been widely used for in vivo detection of neuronal activity in rodents by measuring intracellular neuronal Ca2+ changes. More recently, jRGECO1a, a red shifted GECI, has been reported to detect neuronal Ca2+ activation. This opens the possibility of using dual-color GECIs for simultaneous interrogation of different cell populations. However, there has been no report to compare the functional difference between these two GECIs for in vivo imaging. Here, a comparative study is reported on neuronal responses to sensory stimulation using GCaMP6f and jRGECO1a that were virally delivered into the neurons in the somatosensory cortex of two different groups of animals, respectively. METHODS: GCaMP6f and jRGECO1a GECI were virally delivered to sensory cortex. After 3-4 weeks, the animals were imaged to capture the spatiotemporal changes of neuronal Ca2+ and the hemodynamic responses to forepaw electrical stimulation (0.3 mA, 0.3 ms/pulse, 0.03 Hz). The stimulation-evoked neuronal Ca2+ transients expressed with GCaMP6f or jRGECO1a were recorded during the baseline period and after an acute cocaine administration (1 mg/kg, i.v.). RESULTS: Histology confirmed that the efficiency of jRGECO1a and GCaMP6f expression into the cortical neurons was similar, i.e., 34%±3% and 32.7%±1.6%, respectively. Our imaging in vivo showed that the hemodynamic responses to the stimulation were the same between jRGECO1a and GCaMP6f expressed groups. Although the stimulation-evoked fluorescence change (∆F/F) and the time-to-peak of the neuronal Ca2+ transients were not significantly different between these two indicators, the full-width-half-maximum (FWHM) duration of the ∆F/F rise in the jRGECO1a-expressed group (0.16±0.02 s) was ~50 ms or 46% longer than that of the GCaMP6f group (0.11±0.003 s), indicating a longer recovery time in jRGECO1a than in GCaMP6f transients (P<0.01). This is likely due to the longer off rate of jRGECO1a than that of GCaMP6f. After cocaine, the time-to-peak of Ca2+ transients was delayed and their FWHM duration was prolonged for both expression groups, indicating that these are cocaine's effects on neuronal Ca2+ signaling and not artifacts due to the property differences of the GCEIs. CONCLUSIONS: This study shows that both jRGECO1a and GCaMP6f have sufficient sensitivity for tracking single-stimulation-evoked Ca2+ transients to detect neuronal activities from the brain. Since these GECIs are emitted at the different wavelengths, it will be possible to use them together to characterize the activity of different cell types (e.g., neurons and astrocytes) to study brain activation and brain functional changes in normal or diseased brains. 2021 Quantitative Imaging in Medicine and Surgery. All rights reserved.
Authors: Hod Dana; Boaz Mohar; Yi Sun; Sujatha Narayan; Andrew Gordus; Jeremy P Hasseman; Getahun Tsegaye; Graham T Holt; Amy Hu; Deepika Walpita; Ronak Patel; John J Macklin; Cornelia I Bargmann; Misha B Ahrens; Eric R Schreiter; Vivek Jayaraman; Loren L Looger; Karel Svoboda; Douglas S Kim Journal: Elife Date: 2016-03-24 Impact factor: 8.140
Authors: Lin Tian; S Andrew Hires; Tianyi Mao; Daniel Huber; M Eugenia Chiappe; Sreekanth H Chalasani; Leopoldo Petreanu; Jasper Akerboom; Sean A McKinney; Eric R Schreiter; Cornelia I Bargmann; Vivek Jayaraman; Karel Svoboda; Loren L Looger Journal: Nat Methods Date: 2009-11-08 Impact factor: 28.547
Authors: Nicholas J Beacher; Kayden A Washington; Craig T Werner; Yan Zhang; Giovanni Barbera; Yun Li; Da-Ting Lin Journal: Front Neural Circuits Date: 2021-10-05 Impact factor: 3.492