Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 A reactive peptide interface for site-selective cysteine bioconjugation.

Literature DB >> 33645592

A reactive peptide interface for site-selective cysteine bioconjugation.

Suan Tuang¹, Diomedes Dieppa-Matos, Chi Zhang, Christopher R Shugrue, Peng Dai, Andrei Loas, Bradley L Pentelute.

Abstract

We report aqueous, site-selective modification of proteins using a reactive peptide interface comprising a nine-residue sequence. This interface is the fastest (second-order rate constant of 152 M-1 s-1) catalyst-free, cysteine-based method for modifying proteins available to date, and enables near-quantitative labeling of antibodies in cell lysate.

Entities: Chemical

Mesh：

Substances：

Year: 2021 PMID： 33645592 PMCID： PMC8048384 DOI： 10.1039/d1cc00095k

Source DB: PubMed Journal: Chem Commun (Camb) ISSN： 1359-7345 Impact factor: 6.222

Keyword Cloud
References

35 in total

1. Imaging and manipulating proteins in live cells through covalent labeling.

Authors: Lin Xue; Iuliia A Karpenko; Julien Hiblot; Kai Johnsson
Journal: Nat Chem Biol Date: 2015-11-17 Impact factor: 15.040

2. Recent advances in the construction of antibody-drug conjugates.

Authors: Vijay Chudasama; Antoine Maruani; Stephen Caddick
Journal: Nat Chem Date: 2016-01-04 Impact factor: 24.427

Review 3. Expanding the genetic code.

Authors: Lei Wang; Jianming Xie; Peter G Schultz
Journal: Annu Rev Biophys Biomol Struct Date: 2006

A reactive peptide interface for site-selective cysteine bioconjugation.

1. Imaging and manipulating proteins in live cells through covalent labeling.

2. Recent advances in the construction of antibody-drug conjugates.

Review 3. Expanding the genetic code.

4. AJICAP: Affinity Peptide Mediated Regiodivergent Functionalization of Native Antibodies.

5. Discovery of a 29-Amino-Acid Reactive Abiotic Peptide for Selective Cysteine Arylation.

Review 6. Aromatic rings in chemical and biological recognition: energetics and structures.

7. Conformational Stabilization and Rapid Labeling of a 29-Residue Peptide by a Small Molecule Reaction Partner.

8. Sequence-Specific 2-Cyanobenzothiazole Ligation.

9. A structural and mechanistic study of π-clamp-mediated cysteine perfluoroarylation.

10. Bioorthogonal chemistry: fishing for selectivity in a sea of functionality.