| Literature DB >> 33635272 |
Simona Hankeova1,2, Jakub Salplachta3, Tomas Zikmund3, Michaela Kavkova3, Noémi Van Hul1, Adam Brinek3, Veronika Smekalova3, Jakub Laznovsky3, Feven Dawit4, Josef Jaros5, Vítězslav Bryja2, Urban Lendahl6, Ewa Ellis4, Antal Nemeth7, Björn Fischler4, Edouard Hannezo8, Jozef Kaiser3, Emma Rachel Andersson1,6.
Abstract
Organ function depends on tissues adopting the correct architecture. However, insights into organ architecture are currently hampered by an absence of standardized quantitative 3D analysis. We aimed to develop a robust technology to visualize, digitalize, and segment the architecture of two tubular systems in 3D: double resin casting micro computed tomography (DUCT). As proof of principle, we applied DUCT to a mouse model for Alagille syndrome (Jag1Ndr/Ndr mice), characterized by intrahepatic bile duct paucity, that can spontaneously generate a biliary system in adulthood. DUCT identified increased central biliary branching and peripheral bile duct tortuosity as two compensatory processes occurring in distinct regions of Jag1Ndr/Ndr liver, leading to full reconstitution of wild-type biliary volume and phenotypic recovery. DUCT is thus a powerful new technology for 3D analysis, which can reveal novel phenotypes and provide a standardized method of defining liver architecture in mouse models.Entities:
Keywords: Alagille syndrome; MicroCT; cholangiopathy; human; mouse; physics of living systems; regenerative medicine; resin; stem cells; vasculature
Mesh：
Year: 2021 PMID： 33635272 PMCID： PMC7909953 DOI： 10.7554/eLife.60916
Source DB: PubMed Journal: Elife ISSN： 2050-084X Impact factor: 8.140