Elva I Cortés-Gutiérrez1, Martha I Dávila-Rodríguez2, Homero Sánchez-Dávila3, José Luis Fernández4,5, Carlos García de laVega6, Ricardo M Cerda-Flores7, Jaime Gosálvez6. 1. Faculty of Biological Sciences, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, México. elvairenecortes@yahoo.com.mx. 2. Faculty of Public Health and Nutrition, Universidad Autónoma de Nuevo León, Monterrey, México. 3. Hospital of Obstetrics and Gynecology Dr. Ignacio, Morones Prieto of Instituto Mexicano del Seguro Social, High Specialty Medical Unit No. 23, Monterrey, México. 4. Genetics Unit, Complejo Hospitalario Universitario A Coruña-INIBIC, La Coruña, Spain. 5. Laboratory of Molecular Genetics and Radiology, Oncology Center of Galicia, La Coruña, Spain. 6. Department of Biology, Unidad de Genética, Universidad Autónoma de Madrid (UAM), 20849, Madrid, Spain. 7. Universidad Autónoma de Nuevo León, School of Nursing, Monterrey, México.
Abstract
PURPOSE: This study was aimed to quantify genomic DNA breakages in the cervical epithelium cells of patients diagnosed with different grades of cervical lesions using a quick test based on chromatin dispersion after controlled protein depletion. The association between the progressive stages of cervical dysplasia and the levels of DNA damage, taking into account the presence of papillomavirus human (HPV) infection, was investigated. METHODS: A hospital-based unmatched case-control study was conducted during 2018 with a sample of 78 women grouped according to histological diagnosis as follows: 23 women with low grade-squamous intraepithelial lesion (LG-SIL), 34 women with high grade- squamous intraepithelial lesion (HG-SIL), and three women with cervical carcinoma (CC). In parallel, 15 women without cervical lesions were included as a Control cohort. DNA damage levels in cervical epithelial cells were assessed using the chromatin dispersion test (CDT) and controlled in parallel with DNA breakage detection coupled with florescent in situ hybridization (DBD‒FISH) using whole genomic DNA probes. RESULTS: CDT produces different morphotypes in the cervical epithelium that can be associated with the level of DNA breakage revealed with DBD‒FISH. A significant increase of DNA damage was correlated with the histological progression of the patients and human papillomavirus (HPV) infection. CONCLUSION: The CDT is a simple, accurate and inexpensive morphological bioassay to identify different levels DNA damage that can be associated with the level of abnormal cells present in the cervical epithelium in patients who commonly present HPV infection.
PURPOSE: This study was aimed to quantify genomic DNA breakages in the cervical epithelium cells of patients diagnosed with different grades of cervical lesions using a quick test based on chromatin dispersion after controlled protein depletion. The association between the progressive stages of cervical dysplasia and the levels of DNA damage, taking into account the presence of papillomavirus human (HPV) infection, was investigated. METHODS: A hospital-based unmatched case-control study was conducted during 2018 with a sample of 78 women grouped according to histological diagnosis as follows: 23 women with low grade-squamous intraepithelial lesion (LG-SIL), 34 women with high grade- squamous intraepithelial lesion (HG-SIL), and three women with cervical carcinoma (CC). In parallel, 15 women without cervical lesions were included as a Control cohort. DNA damage levels in cervical epithelial cells were assessed using the chromatin dispersion test (CDT) and controlled in parallel with DNA breakage detection coupled with florescent in situ hybridization (DBD‒FISH) using whole genomic DNA probes. RESULTS:CDT produces different morphotypes in the cervical epithelium that can be associated with the level of DNA breakage revealed with DBD‒FISH. A significant increase of DNA damage was correlated with the histological progression of the patients and human papillomavirus (HPV) infection. CONCLUSION: The CDT is a simple, accurate and inexpensive morphological bioassay to identify different levels DNA damage that can be associated with the level of abnormal cells present in the cervical epithelium in patients who commonly present HPV infection.
Entities:
Keywords:
Chromatin dispersion test; DNA damage; Human papillomavirus; Squamous intraepithelial lesions
Authors: Pablo Barcena; Carmen López-Fernández; Carlos García-Ochoa; Albert Obradors; Valerie Vernaeve; Jaime Gosálvez; Rita Vassena Journal: Syst Biol Reprod Med Date: 2015-08-25 Impact factor: 3.061
Authors: Elva I Cortés-Gutiérrez; Martha I Dávila-Rodríguez; Estela A Zamudio-González; Miguel E Aguado-Barrera; Javier Vargas-Villarreal; Ricardo M Cerda-Flores Journal: Anal Quant Cytol Histol Date: 2010-08 Impact factor: 0.302