OBJECTIVE: To investigate the association between the progressive stage of cervical dysplasia and DNA damage by comet assay. STUDY DESIGN: A hospital-based, unmatched, case-control study was performed. DNA damage levels (none, low, medium and high) in the cervical epithelial cells of 31 women (10 with low grade squamous intraepithelial lesions [LSIL], 10 with high grade [HSIL] and 11 with no cervical lesion) were evaluated using the comet assay. RESULTS: A significant increase in medium DNA damage was observed in women with HSIL (17 +/- 8.9) relative to that in the control women (9 +/- 6.1). A significant increase in high DNA damage was also observed in women with LSIL (23 +/- 15.4) or HSIL (32 +/- 13.1) relative to that in the control women (12 +/- 7.9). CONCLUSION: These findings confirm that the grade of a cervical lesion correlates with the degree of genomic instability.
OBJECTIVE: To investigate the association between the progressive stage of cervical dysplasia and DNA damage by comet assay. STUDY DESIGN: A hospital-based, unmatched, case-control study was performed. DNA damage levels (none, low, medium and high) in the cervical epithelial cells of 31 women (10 with low grade squamous intraepithelial lesions [LSIL], 10 with high grade [HSIL] and 11 with no cervical lesion) were evaluated using the comet assay. RESULTS: A significant increase in medium DNA damage was observed in women with HSIL (17 +/- 8.9) relative to that in the control women (9 +/- 6.1). A significant increase in high DNA damage was also observed in women with LSIL (23 +/- 15.4) or HSIL (32 +/- 13.1) relative to that in the control women (12 +/- 7.9). CONCLUSION: These findings confirm that the grade of a cervical lesion correlates with the degree of genomic instability.
Authors: Elva I Cortés-Gutiérrez; Martha I Dávila-Rodríguez; Homero Sánchez-Dávila; José Luis Fernández; Carlos García de laVega; Ricardo M Cerda-Flores; Jaime Gosálvez Journal: Arch Gynecol Obstet Date: 2021-02-23 Impact factor: 2.344
Authors: Elva I Cortés-Gutiérrez; Fernando Hernández-Garza; Jorge O García-Pérez; Martha I Dávila-Rodríguez; Miguel E Aguado-Barrera; Ricardo M Cerda-Flores Journal: J Biomed Biotechnol Date: 2012-10-03
Authors: Elva I Cortés-Gutiérrez; Brenda L Ortíz-Hernández; Martha I Dávila-Rodríguez; Ricardo M Cerda-Flores; José Luis Fernández; Carmen López-Fernández; Jaime Gosálvez Journal: Int J Mol Sci Date: 2013-02-19 Impact factor: 5.923
Authors: Carlos Alvarez-Moya; Mónica Reynoso-Silva; Alejandro A Canales-Aguirre; José O Chavez-Chavez; Hugo Castañeda-Vázquez; Alfredo I Feria-Velasco Journal: Biomed Res Int Date: 2015-08-03 Impact factor: 3.411