Anastasia Kirillova1, Ekaterina Bunyaeva2, Heidi Van Ranst3, Grigory Khabas2, Maria Farmakovskaya2, Nail Kamaletdinov2, Tatiana Nazarenko2, Aydar Abubakirov2, Gennady Sukhikh2, Johan E J Smitz4. 1. National Medical Research Center for Obstetrics, Gynecology and Perinatology named after V.I.Kulakov, of the Ministry of Healthcare of Russian Federation, Moscow, Russia. anastasia.kirillova@zoho.com. 2. National Medical Research Center for Obstetrics, Gynecology and Perinatology named after V.I.Kulakov, of the Ministry of Healthcare of Russian Federation, Moscow, Russia. 3. Follicle Biology Laboratory (FOBI), Vrije Universiteit Brussel, Brussels, Belgium. 4. Follicle Biology Laboratory (FOBI), Vrije Universiteit Brussel, Brussels, Belgium. johan.smitz@uzbrussel.be.
Abstract
PURPOSE: To investigate the developmental competence of ovarian tissue oocytes from patients with gynecological tumors using a biphasic in vitro maturation system with capacitation (CAPA-IVM) in comparison with standard IVM. METHODS: This sibling pilot study included 210 oocytes in 10 patients with gynecological malignancies. After ovariectomies, ovaries were cut into even halves and immature cumulus-oocyte complexes (COCs) were retrieved from the ovarian tissue. COCs were separately cultured in either a biphasic CAPA-IVM system for 53 h or in standard IVM for 48 h. After IVM, all COCs were denuded and mature oocytes were either vitrified (N=5) or used for ICSI (N=5). Embryos were cultured for 5-6 days and obtained blastocysts were vitrified. RESULTS: Use of the CAPA-IVM system led to a higher meiotic maturation rate in ovarian tissue oocytes (OTO) compared to standard IVM (56 vs 35%, p=0.0045) and had a tendency to result in lower degeneration after IVM. Only the CAPA-IVM method supported blastocyst formation. CONCLUSIONS: The biphasic in vitro maturation system improved the competence of OTO in comparison to the standard IVM method. The study suggests that fertility preservation programs could become more efficient using IVM after capacitation culture.
PURPOSE: To investigate the developmental competence of ovarian tissue oocytes from patients with gynecological tumors using a biphasic in vitro maturation system with capacitation (CAPA-IVM) in comparison with standard IVM. METHODS: This sibling pilot study included 210 oocytes in 10 patients with gynecological malignancies. After ovariectomies, ovaries were cut into even halves and immature cumulus-oocyte complexes (COCs) were retrieved from the ovarian tissue. COCs were separately cultured in either a biphasic CAPA-IVM system for 53 h or in standard IVM for 48 h. After IVM, all COCs were denuded and mature oocytes were either vitrified (N=5) or used for ICSI (N=5). Embryos were cultured for 5-6 days and obtained blastocysts were vitrified. RESULTS: Use of the CAPA-IVM system led to a higher meiotic maturation rate in ovarian tissue oocytes (OTO) compared to standard IVM (56 vs 35%, p=0.0045) and had a tendency to result in lower degeneration after IVM. Only the CAPA-IVM method supported blastocyst formation. CONCLUSIONS: The biphasic in vitro maturation system improved the competence of OTO in comparison to the standard IVM method. The study suggests that fertility preservation programs could become more efficient using IVM after capacitation culture.
Authors: Ingrid Segers; Erlisa Bardhi; Ileana Mateizel; Ellen Van Moer; Rik Schots; Greta Verheyen; Herman Tournaye; Michel De Vos Journal: Hum Reprod Date: 2020-09-01 Impact factor: 6.918
Authors: Mariabeatrice Dal Canto; Fausta Brambillasca; Mario Mignini Renzini; Giovanni Coticchio; Maria Merola; Monia Lain; Elena De Ponti; Rubens Fadini Journal: J Assist Reprod Genet Date: 2012-04-18 Impact factor: 3.412
Authors: Ingrid Segers; Ileana Mateizel; Ellen Van Moer; Johan Smitz; Herman Tournaye; Greta Verheyen; Michel De Vos Journal: J Assist Reprod Genet Date: 2015-08-08 Impact factor: 3.412
Authors: Nazli Akin; Dulama Richani; Xiuhua Liao; Yiqing Zhao; Anamaria-Cristina Herta; Katy Billooye; William A Stocker; David G Mottershead; Craig A Harrison; Johan Smitz; Ellen Anckaert; Robert B Gilchrist Journal: J Assist Reprod Genet Date: 2022-01-04 Impact factor: 3.412
Authors: Michel De Vos; Michaël Grynberg; Tuong M Ho; Ye Yuan; David F Albertini; Robert B Gilchrist Journal: J Assist Reprod Genet Date: 2021-07-03 Impact factor: 3.412
Authors: Huixia Yang; Thomas Kolben; Sarah Meister; Corinna Paul; Julia van Dorp; Sibel Eren; Christina Kuhn; Martina Rahmeh; Sven Mahner; Udo Jeschke; Viktoria von Schönfeldt Journal: Biomedicines Date: 2021-12-14