Unurzul Manlai1,2, Su-Wei Chang2, Shih-Chieh Lee1,3, Wai-Jane Ho1,2, Tai-Hao Hsu1,3, Jaug-Geng Lin4, Chih-Ming Lin2,5, Ying-I Chen2, Shih-Liang Chang1,2,4. 1. College of Biotechnology and Bioresources, Da-Yeh University, Changhua, Taiwan. 2. Department of Medicinal Botanicals and Health Applications, Da-Yeh University, Changhua, Taiwan. 3. Department of Food Science and Biotechnology, Da-Yeh University, Changhua, Taiwan. 4. School of Chinese Medicine, China Medical University, Taichung City, Taiwan. 5. Department of Neurology, Changhua Christian Hospital, Changhua City, Taiwan.
Abstract
Objective: Antrodia cinnamomea (AC), a medicinal mushroom indigenous to Taiwan, exerts various pharmacologic activities. This study compared and evaluated the hypoglycemic effect of treatment with electroacupuncture (EA) combined with AC in steroid-induced insulin-resistant (SIIR) rats. Materials and Methods: Rats were divided into saline, EA, AC, AC+EA, and rosiglitazone (TZD) groups. Plasma-glucose levels were measured in serial blood samples and compared before and after treatment in each group. The levels of signaling proteins-glucose transporter 4, (GLUT4), phosphoinositide 3-kinase (PI3-K), and 5' adenosine monophosphate-activated protein kinase (AMPK)-were analyzed by Western blotting to explore their mechanisms of action. Results: The AC+EA group had reduced plasma-glucose levels at 30 and 60 minutes in SIIR rats, compared to normal rats, and this was better than the EA, AC, and TZD groups at 60 minutes. Furthermore, the signaling protein (GLUT4, PI3-K, and AMPK) levels were increased significantly. Conclusions: These findings showed improved hypoglycemic activity and insulin resistance after EA combined with AC treatment. Therefore, the combined therapy might be a more-effective method than the individual therapies that elevates the expression of the signal proteins, as observed in this study. Copyright 2021, Mary Ann Liebert, Inc., publishers.
Objective: Antrodia cinnamomea (AC), a medicinal mushroom indigenous to Taiwan, exerts various pharmacologic activities. This study compared and evaluated the hypoglycemic effect of treatment with electroacupuncture (EA) combined with AC in steroid-induced insulin-resistant (SIIR) rats. Materials and Methods: Rats were divided into saline, EA, AC, AC+EA, and rosiglitazone (TZD) groups. Plasma-glucose levels were measured in serial blood samples and compared before and after treatment in each group. The levels of signaling proteins-glucose transporter 4, (GLUT4), phosphoinositide 3-kinase (PI3-K), and 5' adenosine monophosphate-activated protein kinase (AMPK)-were analyzed by Western blotting to explore their mechanisms of action. Results: The AC+EA group had reduced plasma-glucose levels at 30 and 60 minutes in SIIR rats, compared to normal rats, and this was better than the EA, AC, and TZD groups at 60 minutes. Furthermore, the signaling protein (GLUT4, PI3-K, and AMPK) levels were increased significantly. Conclusions: These findings showed improved hypoglycemic activity and insulin resistance after EA combined with AC treatment. Therefore, the combined therapy might be a more-effective method than the individual therapies that elevates the expression of the signal proteins, as observed in this study. Copyright 2021, Mary Ann Liebert, Inc., publishers.
Authors: Chi Teng Vong; Hisa Hui Ling Tseng; Yiu Wa Kwan; Simon Ming-Yuen Lee; Maggie Pui Man Hoi Journal: Front Pharmacol Date: 2016-03-21 Impact factor: 5.810