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Literature DB >> 33606973

CRISPR base editor screens identify variant function at scale.

Abstract

Cuella-Martin et al. (2021) and Hanna et al. (2021) showcase CRISPR base editing in large-scale pooled screens in human cells to discover both loss- and gain-of-function variants, enabling protein structure/function insights and clinical variant interpretation.

Entities: Chemical

Mesh：

Year: 2021 PMID： 33606973 PMCID： PMC9017793 DOI： 10.1016/j.molcel.2021.01.036

Source DB: PubMed Journal: Mol Cell ISSN： 1097-2765 Impact factor: 19.328

Keyword Cloud
References

10 in total

1. High-throughput Phenotyping of Lung Cancer Somatic Mutations.

Authors: Alice H Berger; Angela N Brooks; Xiaoyun Wu; Yashaswi Shrestha; Candace Chouinard; Federica Piccioni; Mukta Bagul; Atanas Kamburov; Marcin Imielinski; Larson Hogstrom; Cong Zhu; Xiaoping Yang; Sasha Pantel; Ryo Sakai; Jacqueline Watson; Nathan Kaplan; Joshua D Campbell; Shantanu Singh; David E Root; Rajiv Narayan; Ted Natoli; David L Lahr; Itay Tirosh; Pablo Tamayo; Gad Getz; Bang Wong; John Doench; Aravind Subramanian; Todd R Golub; Matthew Meyerson; Jesse S Boehm
Journal: Cancer Cell Date: 2016-07-28 Impact factor: 31.743

2. Perturbing proteomes at single residue resolution using base editing.

Authors: Philippe C Després; Alexandre K Dubé; Motoaki Seki; Nozomu Yachie; Christian R Landry
Journal: Nat Commun Date: 2020-04-20 Impact factor: 14.919

3. Massively parallel assessment of human variants with base editor screens.

Authors: Ruth E Hanna; Mudra Hegde; Christian R Fagre; Peter C DeWeirdt; Annabel K Sangree; Zsofia Szegletes; Audrey Griffith; Marissa N Feeley; Kendall R Sanson; Yossef Baidi; Luke W Koblan; David R Liu; James T Neal; John G Doench
Journal: Cell Date: 2021-02-18 Impact factor: 41.582

4. Functional interrogation of DNA damage response variants with base editing screens.

Authors: Raquel Cuella-Martin; Samuel B Hayward; Xiao Fan; Xiao Chen; Jen-Wei Huang; Angelo Taglialatela; Giuseppe Leuzzi; Junfei Zhao; Raul Rabadan; Chao Lu; Yufeng Shen; Alberto Ciccia
Journal: Cell Date: 2021-02-18 Impact factor: 41.582

5. Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage.

Authors: Nicole M Gaudelli; Alexis C Komor; Holly A Rees; Michael S Packer; Ahmed H Badran; David I Bryson; David R Liu
Journal: Nature Date: 2017-10-25 Impact factor: 49.962

6. Multiplex assessment of protein variant abundance by massively parallel sequencing.

Authors: Kenneth A Matreyek; Lea M Starita; Jason J Stephany; Beth Martin; Melissa A Chiasson; Vanessa E Gray; Martin Kircher; Arineh Khechaduri; Jennifer N Dines; Ronald J Hause; Smita Bhatia; William E Evans; Mary V Relling; Wenjian Yang; Jay Shendure; Douglas M Fowler
Journal: Nat Genet Date: 2018-05-21 Impact factor: 38.330

CRISPR base editor screens identify variant function at scale.

1. High-throughput Phenotyping of Lung Cancer Somatic Mutations.

2. Perturbing proteomes at single residue resolution using base editing.

3. Massively parallel assessment of human variants with base editor screens.

4. Functional interrogation of DNA damage response variants with base editing screens.

5. Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage.

6. Multiplex assessment of protein variant abundance by massively parallel sequencing.

7. A CRISPR-based base-editing screen for the functional assessment of BRCA1 variants.

8. Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage.

9. Accurate classification of BRCA1 variants with saturation genome editing.

10. Single-cell analysis of a mutant library generated using CRISPR-guided deaminase in human melanoma cells.