Literature DB >> 336007

The rapid concentration and purification of influenza virus from allantoic fluid.

J T Heyward, R A Klimas, M D Stapp, J F Obijeski.   

Abstract

Influenza virus was quantitatively recovered from infectious allantoic fluid by precipitation with 8 percent (wt/vol) polyethylene glycol 6000 (PEG-6000). Virus concentrated by PEG-6000 treatment was rapidly purified by gel permeation chromatography through controlled pore glass beads. The purity of virus preparations achieved by this method, when judged by three independent criteria, was comparable to virus preparations purified by conventional density gradient procedures.

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Year:  1977        PMID： 336007     DOI： 10.1007/bf01314484

Source DB:  PubMed          Journal:  Arch Virol        ISSN： 0304-8608            Impact factor:   2.574


  21 in total

1.  Polyacrylamide gel electrophoresis of fowlpox and vaccinia virus proteins.

Authors:  J F Obijeski; E L Palmer; L G Gafford; C C Randall
Journal:  Virology       Date:  1973-02       Impact factor: 3.616

2.  Concentration and purification of vesicular stomatitis virus by polyethylene glycol "precipitation".

Authors:  J McSharry; R Benzinger
Journal:  Virology       Date:  1970-03       Impact factor: 3.616

3.  Application of permeation chromatography on controlled-pore glass in the purification of plant viruses.

Authors:  K Marcinka
Journal:  Acta Virol       Date:  1972-06       Impact factor: 1.162

4.  Plaque formation by influenza viruses in the presence of trypsin.

Authors:  G Appleyard; H B Maber
Journal:  J Gen Virol       Date:  1974-12       Impact factor: 3.891

5.  Large-scale preparations of viruses by steric chromatography on columns of controlled pore glass. Phi-X174, M13, M12, Q-beta and T4 bacteriophages.

Authors:  H H Gschwender; W Haller; P H Hofschneider
Journal:  Biochim Biophys Acta       Date:  1969-10-22

6.  A single equation relating molecular weight, pore-size, and elution coefficient in the controlled pore glass chromatography of protein-sodium dodecyl sulfate complexes.

Authors:  W Haller
Journal:  J Chromatogr       Date:  1973-10-10

7.  Purification of large quantities of influenza virus by density gradient centrifugation.

Authors:  C B Reimer; R S Baker; R M Van Frank; T E Newlin; G B Cline; N G Anderson
Journal:  J Virol       Date:  1967-12       Impact factor: 5.103

8.  A simple method for concentration of arboviruses propagated in tissue culture.

Authors:  M Horzinek
Journal:  Am J Trop Med Hyg       Date:  1969-07       Impact factor: 2.345

9.  The isolation of plant viruses by means of "simple" coacervates.

Authors:  R Leberman
Journal:  Virology       Date:  1966-11       Impact factor: 3.616

10.  Evaluation of some methods of concentration and purification of influenza virus.

Authors:  N VEERARAGHAVAN; T SREEVALSAN
Journal:  Bull World Health Organ       Date:  1961       Impact factor: 9.408
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  14 in total

1.  Ultrastructural morphometric investigation of early lesions in the pulmonary alveolar region of pigs during experimental swine influenza infection.

Authors:  G C Winkler; N F Cheville
Journal:  Am J Pathol       Date:  1986-03       Impact factor: 4.307

2.  An optimized molecular method for detection of influenza A virus using improved generic primers and concentration of the viral genomic RNA and nucleoprotein complex.

Authors:  Ji-Woon Kim; Chung-Young Lee; Thanh Trung Nguyen; Il-Hwan Kim; Hyuk-Joon Kwon; Jae-Hong Kim
Journal:  J Vet Diagn Invest       Date:  2019-02-22       Impact factor: 1.279

3.  Polyethylene glycol precipitation for recovery of pathogenic viruses, including hepatitis A virus and human rotavirus, from oyster, water, and sediment samples.

Authors:  G D Lewis; T G Metcalf
Journal:  Appl Environ Microbiol       Date:  1988-08       Impact factor: 4.792

4.  Mutational analysis of the conserved motifs of influenza A virus polymerase basic protein 1.

Authors:  S K Biswas; D P Nayak
Journal:  J Virol       Date:  1994-03       Impact factor: 5.103

5.  Large-scale purification of Japanese encephalitis virus from infected mouse brain for preparation of vaccine.

Authors:  C Aizawa; S Hasegawa; C Chih-Yuan; I Yoshioka
Journal:  Appl Environ Microbiol       Date:  1980-01       Impact factor: 4.792

6.  Simple method for the concentration of influenza virus from allantoic fluid on microporous filters.

Authors:  S M Goyal; H Hanssen; C P Gerba
Journal:  Appl Environ Microbiol       Date:  1980-03       Impact factor: 4.792

7.  Possible waterborne transmission and maintenance of influenza viruses in domestic ducks.

Authors:  D D Markwell; K F Shortridge
Journal:  Appl Environ Microbiol       Date:  1982-01       Impact factor: 4.792

8.  Complete metal ion requirement of influenza virus N1 neuraminidases. Brief report.

Authors:  S M Carroll; J C Paulson
Journal:  Arch Virol       Date:  1982       Impact factor: 2.574

9.  Incorporation of a proteotyping approach using mass spectrometry for surveillance of influenza virus in cell-cultured strains.

Authors:  Neil D Fernandes; Kevin M Downard
Journal:  J Clin Microbiol       Date:  2013-11-13       Impact factor: 5.948

10.  Host gene expression signatures discriminate between ferrets infected with genetically similar H1N1 strains.

Authors:  Karl Ljungberg; Alexis McBrayer; Jeremy V Camp; Yong-Kyu Chu; Ronald Tapp; Diana L Noah; Sheila Grimes; Mary L Proctor; Peter Liljeström; Colleen B Jonsson; Carl E Bruder
Journal:  PLoS One       Date:  2012-07-13       Impact factor: 3.240
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