Literature DB >> 33584145

Analysis of short-term variation and long-term drift during reagent kit lot change in an NABL accredited clinical biochemistry laboratory.

Vivek Ambade1, Pratibha Misra1, Yaongamphi Vashum1, Mukul Sharma1, Bhasker Mukherjee1, Kapil Bhatia1, Manoj Puliyath1, Ponnaiah Rasu1, Prakash Berthwal Indra1, Madathan Kandi Sibin1.   

Abstract

BACKGROUND: Kit lot change in clinical biochemistry labs leads to variations in patient results. This study planned to identify variations during 60 reagent lot changes in our laboratory during the period from June 2018 to May 2019.
METHODS: A statistical analysis was performed to identify the difference between patient samples results variations and QC results. The long term drift was analyzed using a regression test.
RESULTS: There was a significant difference between the patient and QC results in 16.7% of reagent lot changes. Moreover, the extent of variation in QC results was 3.3%. No long-term drift was seen in three analytes which were studied using regression analysis.
CONCLUSIONS: Our results showed that, during reagent kit lot change, along with QC material, the patient samples should also be run in order to identify the variation. However, this practice is presently ignored by most of the laboratories. There was no accumulated effect in our laboratory due to reagent kit lot change. 2021 Vivek Ambade, Pratibha Misra, Yaongamphi Vashum, Mukul Sharma, Bhasker Mukherjee, Kapil Bhatia, Manoj Puliyath, Ponnaiah Rasu, Prakash Berthwal Indra, Madathan Kandi Sibin, published by CEON/CEES.

Entities:  

Keywords:  NABL; clinical chemistry; long term drift; quality control; reagent lot change; short term variation

Year:  2021        PMID: 33584145      PMCID: PMC7857852          DOI: 10.5937/jomb0-25597

Source DB:  PubMed          Journal:  J Med Biochem        ISSN: 1452-8266            Impact factor:   3.402


  11 in total

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Review 5.  Lot-to-Lot Variation.

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6.  Failure of current laboratory protocols to detect lot-to-lot reagent differences: findings and possible solutions.

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8.  Assessment of the Abbott IMx assay system for the measurement of human chorionic gonadotropin levels in the treatment of ectopic pregnancy.

Authors:  D B Goodman; M Bulley; M Hendricks; M Senior
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9.  Statistical validation of reagent lot change in the clinical chemistry laboratory can confer insights on good clinical laboratory practice.

Authors:  Min-Chul Cho; So Young Kim; Tae-Dong Jeong; Woochang Lee; Sail Chun; Won-Ki Min
Journal:  Ann Clin Biochem       Date:  2014-02-04       Impact factor: 2.057

10.  Analysis of reagent lot-to-lot comparability tests in five immunoassay items.

Authors:  Hyun Soo Kim; Hee Jung Kang; Dong Hee Whang; Seong Gyu Lee; Min Jeong Park; Ji-Young Park; Kyu Man Lee
Journal:  Ann Clin Lab Sci       Date:  2012       Impact factor: 1.256

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