Zheng Wu1, Shujuan Cheng1, Shaoping Wang1, Wenzheng Li1, Jinghua Liu2. 1. Department of 28 Division of Cardiovascular, Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing 100029, China. 2. Department of 28 Division of Cardiovascular, Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing 100029, China. Electronic address: liujinghua3345@outlook.com.
Abstract
OBJECTIVE: MicroRNAs (miRNAs) have engaged in the progression of myocardial infarction (MI). Nevertheless, the mechanism of miR-150-5p in MI is still in its infancy. Therefore, the present study was set out to investigate the effect of bone marrow mesenchymal stem cells derived exosomes (BMSCs-Exo) and miR-150-5p in MI via regulating B-cell lymphoma-associated X (Bax). METHODS: BMSCs-Exo were isolated and extracted, and exosomes with miR-150-5p agomir or antagomir was constructed. Then, a mouse MI model was induced by ligation of left anterior descending coronary artery. Mice were injected with exosomes and miR-150-5p agomir/antagomir to detect cardiac function, pathological changes, and apoptosis rate of cardiomyocytes. miR-150-5p and Bax expression in myocardial tissues were tested. The targeting relationship between miR-150-5p and Bax was verified. RESULTS: BMSCs and exosomes were successfully extracted. BMSCs-derived exosomal miR-150-5p improved cardiac function, alleviated pathological changes of myocardium, decreased apoptosis rate of cardiomyocytes in MI mice. miR-150-5p expression was reduced and Bax expression was elevated in myocardial tissues of MI mice, while exosomes raised miR-150-5p expression and reduced Bax expression in MI mice. miR-150-5p was found to target Bax. CONCLUSION: On all accounts, the present study provides evidence that BMSCs-derived exosomal miR-150-5p attenuates apoptosis of cardiomyocytes and improves cardiac function of MI mice via targeting Bax.
OBJECTIVE: MicroRNAs (miRNAs) have engaged in the progression of myocardial infarction (MI). Nevertheless, the mechanism of miR-150-5p in MI is still in its infancy. Therefore, the present study was set out to investigate the effect of bone marrow mesenchymal stem cells derived exosomes (BMSCs-Exo) and miR-150-5p in MI via regulating B-cell lymphoma-associated X (Bax). METHODS: BMSCs-Exo were isolated and extracted, and exosomes with miR-150-5p agomir or antagomir was constructed. Then, a mouse MI model was induced by ligation of left anterior descending coronary artery. Mice were injected with exosomes and miR-150-5p agomir/antagomir to detect cardiac function, pathological changes, and apoptosis rate of cardiomyocytes. miR-150-5p and Bax expression in myocardial tissues were tested. The targeting relationship between miR-150-5p and Bax was verified. RESULTS: BMSCs and exosomes were successfully extracted. BMSCs-derived exosomal miR-150-5p improved cardiac function, alleviated pathological changes of myocardium, decreased apoptosis rate of cardiomyocytes in MI mice. miR-150-5p expression was reduced and Bax expression was elevated in myocardial tissues of MI mice, while exosomes raised miR-150-5p expression and reduced Bax expression in MI mice. miR-150-5p was found to target Bax. CONCLUSION: On all accounts, the present study provides evidence that BMSCs-derived exosomal miR-150-5p attenuates apoptosis of cardiomyocytes and improves cardiac function of MI mice via targeting Bax.