SIGNIFICANCE: Raman spectroscopy has been developed for surgical guidance applications interrogating live tissue during tumor resection procedures to detect molecular contrast consistent with cancer pathophysiological changes. To date, the vibrational spectroscopy systems developed for medical applications include single-point measurement probes and intraoperative microscopes. There is a need to develop systems with larger fields of view (FOVs) for rapid intraoperative cancer margin detection during surgery. AIM: We design a handheld macroscopic Raman imaging system for in vivo tissue margin characterization and test its performance in a model system. APPROACH: The system is made of a sterilizable line scanner employing a coherent fiber bundle for relaying excitation light from a 785-nm laser to the tissue. A second coherent fiber bundle is used for hyperspectral detection of the fingerprint Raman signal over an area of 1 cm2. Machine learning classifiers were trained and validated on porcine adipose and muscle tissue. RESULTS: Porcine adipose versus muscle margin detection was validated ex vivo with an accuracy of 99% over the FOV of 95 mm2 in ∼3 min using a support vector machine. CONCLUSIONS: This system is the first large FOV Raman imaging system designed to be integrated in the workflow of surgical cancer resection. It will be further improved with the aim of discriminating brain cancer in a clinically acceptable timeframe during glioma surgery.
SIGNIFICANCE: Raman spectroscopy has been developed for surgical guidance applications interrogating live tissue during tumor resection procedures to detect molecular contrast consistent with cancer pathophysiological changes. To date, the vibrational spectroscopy systems developed for medical applications include single-point measurement probes and intraoperative microscopes. There is a need to develop systems with larger fields of view (FOVs) for rapid intraoperative cancer margin detection during surgery. AIM: We design a handheld macroscopic Raman imaging system for in vivo tissue margin characterization and test its performance in a model system. APPROACH: The system is made of a sterilizable line scanner employing a coherent fiber bundle for relaying excitation light from a 785-nm laser to the tissue. A second coherent fiber bundle is used for hyperspectral detection of the fingerprint Raman signal over an area of 1 cm2. Machine learning classifiers were trained and validated on porcine adipose and muscle tissue. RESULTS: Porcine adipose versus muscle margin detection was validated ex vivo with an accuracy of 99% over the FOV of 95 mm2 in ∼3 min using a support vector machine. CONCLUSIONS: This system is the first large FOV Raman imaging system designed to be integrated in the workflow of surgical cancer resection. It will be further improved with the aim of discriminating brain cancer in a clinically acceptable timeframe during glioma surgery.
Authors: Minbiao Ji; Daniel A Orringer; Christian W Freudiger; Shakti Ramkissoon; Xiaohui Liu; Darryl Lau; Alexandra J Golby; Isaiah Norton; Marika Hayashi; Nathalie Y R Agar; Geoffrey S Young; Cathie Spino; Sandro Santagata; Sandra Camelo-Piragua; Keith L Ligon; Oren Sagher; X Sunney Xie Journal: Sci Transl Med Date: 2013-09-04 Impact factor: 17.956
Authors: Susan M Chang; Ian F Parney; Michael McDermott; Fred G Barker; Meic H Schmidt; Wei Huang; Edward R Laws; Kevin O Lillehei; Mark Bernstein; Henry Brem; Andrew E Sloan; Mitchel Berger Journal: J Neurosurg Date: 2003-06 Impact factor: 5.115
Authors: Willie C Zúñiga; Veronica Jones; Sarah M Anderson; Alex Echevarria; Nathaniel L Miller; Connor Stashko; Daniel Schmolze; Philip D Cha; Ragini Kothari; Yuman Fong; Michael C Storrie-Lombardi Journal: Sci Rep Date: 2019-10-10 Impact factor: 4.379
Authors: Merisa L Piper; Jasmine Wong; Kelly Fahrner-Scott; Cheryl Ewing; Michael Alvarado; Laura J Esserman; Rita A Mukhtar Journal: NPJ Breast Cancer Date: 2019-09-06