Ji Qi1, Leyan Feng2, Xiaoyan Zhang3, Haoke Zhang1, Liwen Huang2, Yutong Zhou2, Zheng Zhao1, Xingchen Duan3, Fei Xu2, Ryan T K Kwok1,4, Jacky W Y Lam1,4, Dan Ding3, Xue Xue5, Ben Zhong Tang6,7,8. 1. Department of Chemistry, The Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Institute for Advanced Study, Department of Chemical and Biological Engineering and Institute of Molecular Functional Materials, The Hong Kong University of Science and Technology, Kowloon, Hong Kong, China. 2. State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Nankai University, Haihe Education Park, Tianjin, China. 3. State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, and College of Life Sciences, Nankai University, Tianjin, China. 4. HKUST-Shenzhen Research Institute, Nanshan, Shenzhen, China. 5. State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Nankai University, Haihe Education Park, Tianjin, China. xuexue@nankai.edu.cn. 6. Department of Chemistry, The Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Institute for Advanced Study, Department of Chemical and Biological Engineering and Institute of Molecular Functional Materials, The Hong Kong University of Science and Technology, Kowloon, Hong Kong, China. tangbenz@ust.hk. 7. HKUST-Shenzhen Research Institute, Nanshan, Shenzhen, China. tangbenz@ust.hk. 8. NSFC Centre for Luminescence from Molecular Aggregates, SCUT-HKUST Joint Research Institute, State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Guangzhou, China. tangbenz@ust.hk.
Abstract
Nitric oxide (NO) is an important signaling molecule overexpressed in many diseases, thus the development of NO-activatable probes is of vital significance for monitoring related diseases. However, sensitive photoacoustic (PA) probes for detecting NO-associated complicated diseases (e.g., encephalitis), have yet to be developed. Herein, we report a NO-activated PA probe for in vivo detection of encephalitis by tuning the molecular geometry and energy transformation processes. A strong donor-acceptor structure with increased conjugation can be obtained after NO treatment, along with the active intramolecular motion, significantly boosting "turn-on" near-infrared PA property. The molecular probe exhibits high specificity and sensitivity towards NO over interfering reactive species. The probe is capable of detecting and differentiating encephalitis in different severities with high spatiotemporal resolution. This work will inspire more insights into the development of high-performing activatable PA probes for advanced diagnosis by making full use of intramolecular motion and energy transformation processes.
lass="Chemical">palass="Chemical">n class="Chemical">lass="Chemical">n class="Chemical">Nitric oxide (NO) is an important signaling molecule overexpressed in many diseases, thus the development of NO-activatable probes is of vital significance for monitoring related diseases. However, sensitive photoacoustic (PA) probes for detecting NO-associated complicated diseases (e.g., palass="Chemical">n>n class="Disease">encephalitis), have yet to be developed. Herein, we report a NO-activated PA probe for in vivo detection of encephalitis by tuning the molecular geometry and energy transformation processes. A strong donor-acceptor structure with increased conjugation can be obtained after NO treatment, along with the active intramolecular motion, significantly boosting "turn-on" near-infrared PA property. The molecular probe exhibits high specificity and sensitivity towards NO over interfering reactive species. The probe is capable of detecting and differentiating encephalitis in different severities with high spatiotemporal resolution. This work will inspire more insights into the development of high-performing activatable PA probes for advanced diagnosis by making full use of intramolecular motion and energy transformation processes.
Authors: Wojciech Danowski; Thomas van Leeuwen; Shaghayegh Abdolahzadeh; Diederik Roke; Wesley R Browne; Sander J Wezenberg; Ben L Feringa Journal: Nat Nanotechnol Date: 2019-03-18 Impact factor: 39.213
Authors: Zheng Zhao; Chao Chen; Wenting Wu; Fenfen Wang; Lili Du; Xiaoyan Zhang; Yu Xiong; Xuewen He; Yuanjing Cai; Ryan T K Kwok; Jacky W Y Lam; Xike Gao; Pingchuan Sun; David Lee Phillips; Dan Ding; Ben Zhong Tang Journal: Nat Commun Date: 2019-02-15 Impact factor: 14.919
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