Literature DB >> 33573151

Production of the HBc Protein from Different HBV Genotypes in E. coli. Use of Reassociated HBc VLPs for Packaging of ss- and dsRNA.

Ivars Petrovskis1, Ilva Lieknina1, Andris Dislers1, Juris Jansons1, Janis Bogans1, Inara Akopjana1, Jelena Zakova1, Irina Sominskaya1.   

Abstract

The core proteins (HBc) of the hepatitis B virus (HBV) genotypes A, B, C, D, E, F, and G were cloned and expressed in Escherichia coli (E. coli), and HBc-formed virus-like particles (VLPs) were purified with ammonium sulfate precipitation, gel filtration, and ion exchange chromatography (IEX). The best VLP yield was found for the HBc of the HBV genotypes D and G. For the HBc of the HBV genotypes D, F, and G, the possibility of dissociation and reassociation maintaining the native HBc structure was demonstrated. Single-stranded (ss) and double-stranded (ds) ribonucleic acid (RNA) was successfully packed into HBc VLPs for the HBV genotypes D and G.

Entities:  

Keywords:  HBV genotypes; HBcAg expression; encapsidation

Year:  2021        PMID： 33573151      PMCID： PMC7912224          DOI： 10.3390/microorganisms9020283

Source DB:  PubMed          Journal:  Microorganisms        ISSN： 2076-2607


  46 in total

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  1 in total

1.  Effects of Different Lengths of a Nucleic Acid Binding Region and Bound Nucleic Acids on the Phase Behavior and Purification Process of HBcAg Virus-Like Particles.

Authors:  Angela Valentic; Jakob Müller; Jürgen Hubbuch
Journal:  Front Bioeng Biotechnol       Date:  2022-07-01
  1 in total

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