You-Ying Chen1,2, Yi-Lin Zhang2,3, Gene-Hsiang Lee4, Lun Kelvin Tsou5, Mingzi M Zhang6, Hsing-Pang Hsieh5,7,8, Jih-Jung Chen9, Chou-Yuan Ko10,11, Zhi-Hong Wen1,12, Ping-Jyun Sung1,2,3,13,14. 1. Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804201, Taiwan. 2. National Museum of Marine Biology and Aquarium, Pingtung 944401, Taiwan. 3. Graduate Institute of Marine Biology, National Dong Hwa University, Pingtung 944401, Taiwan. 4. Instrumentation Center, National Taiwan University, Taipei 106319, Taiwan. 5. Institute of Biotechnology and Pharmaceutical Research, National Health Research Institutes, Miaoli 350401, Taiwan. 6. Institute of Molecular and Genomic Medicine, National Health Research Institutes, Miaoli 350401, Taiwan. 7. Department of Chemistry, National Tsing Hua University, Hsinchu 300044, Taiwan. 8. Biomedical Translation Research Center, Academia Sinica, Taipei 115202, Taiwan. 9. Faculty of Pharmacy, School of Pharmaceutical Sciences, National Yang Ming Chiao Tung University, Taipei 112304, Taiwan. 10. Division of Gastroenterology, Department of Internal Medicine, Kaohsiung Armed Forces General Hospital, Kaohsiung 802301, Taiwan. 11. Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung 804201, Taiwan. 12. Institute of BioPharmaceutical Sciences, National Sun Yat-sen University, Kaohsiung 804201, Taiwan. 13. Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung 404394, Taiwan. 14. Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807378, Taiwan.
Abstract
Briareum stechei is proven to be a rich source of 3,8-cyclized cembranoids (briarane) with a bicyclo[8.4.0] carbon core. In the present study, four previously unreported briaranes, briarenols W-Z (1-4), along with solenolide A (5), briarenolide M (6), briaexcavatolide F (7), and brianolide (8), were isolated and characterized through spectroscopic analysis, and the absolute configuration of 8 was corroborated by a single-crystal x-ray diffraction analysis. Briaranes 2 and 5 were found to induce significant inflammatory activity in lipopolysaccharide (LPS)-induced RAW 264.7 mouse macrophage cells by enhancing the expression of the inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) proteins.
Briareum stechei is proven to be a rich source of pan class="Chemical">3,8-cyclized cembranoids (briarane) with a bicyclo[8.4.0] carbon core. In the present study, four previously unreported briaranes, briarenols W-Z (1-4), along with solenolide A (5), briarenolide M (6), briaexcavatolide F (7), and brianolide (8), were isolated and characterized through spectroscopic analysis, and the absolute configuration of 8 was corroborated by a single-crystal x-ray diffraction analysis. Briaranes 2 and 5 were found to induce significant inflammatory activity in lipopolysaccharide (LPS)-induced RAW 264.7mouse macrophage cells by enhancing the expression of the inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) proteins.
Authors: Miguel C Leal; Ricardo Calado; Christopher Sheridan; Andrea Alimonti; Ronald Osinga Journal: Trends Biotechnol Date: 2013-07-16 Impact factor: 19.536
Authors: J Kobayashi; J F Cheng; H Nakamura; Y Ohizumi; Y Tomotake; T Matsuzaki; K J Grace; R S Jacobs; Y Kato; L S Brinen Journal: Experientia Date: 1991-05-15