Literature DB >> 3357213

In vitro synthesis of infectious retroviral RNA.

F Flamant1, J A Sorge.   

Abstract

A plasmid was constructed in which a T7 RNA polymerase promoter was placed upstream of a recombinant amphotropic retrovirus genome containing a selectable neomycin resistance gene. To test the infectivity of the RNA produced by T7 RNA polymerase in vitro, the RNA was microinjected into the nuclei of psi 2 packaging cells. Infectious particles conferring G418 resistance were released.

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Year:  1988        PMID： 3357213      PMCID： PMC253241     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  12 in total

1.  Microinjection analysis of envelope-glycoprotein messenger activities of avian leukosis viral RNAs.

Authors:  D W Stacey; V G Allfrey; H Hanafusa
Journal:  Proc Natl Acad Sci U S A       Date:  1977-04       Impact factor: 11.205

2.  Virus infection of murine teratocarcinoma stem cell lines.

Authors:  N M Teich; R A Weiss; G R Martin; D R Lowy
Journal:  Cell       Date:  1977-12       Impact factor: 41.582

3.  Reverse transcription of retroviral genomes: mutations in the terminal repeat sequences.

Authors:  L I Lobel; S P Goff
Journal:  J Virol       Date:  1985-02       Impact factor: 5.103

4.  New RNA polymerase from Escherichia coli infected with bacteriophage T7.

Authors:  M Chamberlin; J McGrath; L Waskell
Journal:  Nature       Date:  1970-10-17       Impact factor: 49.962

5.  Expression of a foreign gene in myeloid and lymphoid cells derived from multipotent haematopoietic precursors.

Authors:  G Keller; C Paige; E Gilboa; E F Wagner
Journal:  Nature       Date:  1985 Nov 14-20       Impact factor: 49.962

6.  Construction of a retrovirus packaging mutant and its use to produce helper-free defective retrovirus.

Authors:  R Mann; R C Mulligan; D Baltimore
Journal:  Cell       Date:  1983-05       Impact factor: 41.582

7.  Activation of a cellular onc gene by promoter insertion in ALV-induced lymphoid leukosis.

Authors:  W S Hayward; B G Neel; S M Astrin
Journal:  Nature       Date:  1981-04-09       Impact factor: 49.962

8.  Microinjection of mRNA and other macromolecules into living cells.

Authors:  D W Stacey
Journal:  Methods Enzymol       Date:  1981       Impact factor: 1.600

9.  Precise localization of m6A in Rous sarcoma virus RNA reveals clustering of methylation sites: implications for RNA processing.

Authors:  S E Kane; K Beemon
Journal:  Mol Cell Biol       Date:  1985-09       Impact factor: 4.272

10.  Amphotropic retrovirus vector system for human cell gene transfer.

Authors:  J Sorge; D Wright; V D Erdman; A E Cutting
Journal:  Mol Cell Biol       Date:  1984-09       Impact factor: 4.272
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