A comparison of the inflammatory response following autologous compared with allogenic islet cell transplantation.

BACKGROUND: The initial response to islet transplantation and the subsequent acute inflammation is responsible for significant attrition of islets following both autologous and allogenic procedures. This multicentre study compares this inflammatory response using cytokine profiles and complement activation.
METHODS: Inflammatory cytokine and complement pathway activity were examined in two cohorts of patients undergoing total pancreatectomy followed either by autologous (n=11) or allogenic (n=6) islet transplantation. Two patients who underwent total pancreatectomy alone (n=2) served as controls.
RESULTS: The peak of cytokine production occurred immediately following induction of anaesthesia and during surgery. There was found to be a greater elevation of the following cytokines: TNF-alpha (P<0.01), MCP-1 (P=0.0013), MIP-1α (P=0.001), MIP-1β (P=0.00020), IP-10 (P=0.001), IL-8 (P=0.004), IL-1α (P=0.001), IL-1ra (0.0018), IL-10 (P=0.001), GM-CSF (P=0.001), G-CSF (P=0.0198), and Eotaxin (P=0.01) in the allogenic group compared to autografts and controls. Complement activation and consumption was observed in all three pathways, and there were no significant differences in between the groups although following allogenic transplantation ∆IL-10 and ∆VEGF levels were significantly elevated those patients who became insulin-independent compared with those who were insulin-dependent.
CONCLUSIONS: The cytokine profiles following islet transplantation suggests a significantly greater acute inflammatory response following allogenic islet transplantation compared with auto-transplantation although a significant, non-specific inflammatory response occurs following both forms of islet transplantation. 2021 Annals of Translational Medicine. All rights reserved.

Introduction

Allogenic islet cell transplantation is predominantly used to treat insulin dependent Type 1 diabetes mellitus, whereas autologous islet transplantation is employed following total pancreatectomy for unremitting pain in chronic pancreatitis. Both procedures are broadly similar conceptually but have key differences related to the islet procurement, islet isolation procedures and their immunobiology (1).

Allogenic islet transplantation is now established as a treatment for insulin-dependent diabetes mellitus and the continued development of anti-rejection therapies together with refinements in the islet isolation process have facilitated a progressive improvement in clinical outcomes (1-7). These improvements include islet isolation protocols, rates of insulin independence and prevention of hypoglycaemic unawareness (8). In addition, although the function of solid organ allografts declines over time, islet transplants generally continue to remain functional as evidenced by the continued production of C-peptide (9,10).

Between 1999 and 2002 islet transplant protocols were predominately based on the Edmonton Protocol which utilised an interleukin-2 receptor antagonist, an mTOR inhibitor and a calcineurin inhibitor (CNI) (11). After 2002 there was a shift towards the use of induction therapy with T-cell depleting agents and the judicious addition of tumour necrosis factor-α (TNF-α) inhibitor and these developments were introduced into clinical practice over the next decade (12,13). An mTOR inhibitor or an inosine monophosphate dehydrogenase inhibitor (IMPDH) combined with a CNI are now commonly used as maintenance therapy in allogenic islet transplantation (14).

Autologous islet cell transplantation is generally performed following a total pancreatectomy for the treatment of intractable pain in patients with chronic pancreatitis provided that they are not diabetic. Insulin independence following autologous islet transplantation is quite variable and although it declines over time falling from 46% at 5 years to 10% by 8 years (15). This is significantly higher than respective figures for allogenic transplantation. It is likely that differences in immunogenicity between allogenic and autologous infusions of islets may account for the observed differences in insulin independence. Both forms of islet cell transplantation stimulate an immediate innate immune response but autologous islets do not undergo ‘rejection’ or demonstrate susceptibility to the harmful effects of autoimmunity and consequently do not require immunosuppressive therapy. Another important difference is that maintenance immunosuppression following allogenic transplantation generally requires the use of diabetogenic agents which further reduces the prospect of insulin independence in the long term (16).

An important area of islet transplantation research concerns the initial phase following transplantation when the infusion of the islet preparation may precipitate an acute inflammatory response with the activation of complement. This inflammatory response has the potential to significantly damage the infused islets, inhibiting their implantation and resulting in a reduced effective islet cell mass (17-24). Indeed, it has been suggested that as many as 50–60% of infused allogenic islet cells may be lost in the immediate post-transplant period. This significant initial loss of islet cell mass is one of the contributing factors to the almost ubiquitous requirement for multiple donor pancreata for allogenic transplantation to achieve an adequate islet cell mass and consequent medium and long-term insulin independence.

There are numerous reports contrasting the inflammatory response following autologous and allogenic islet cell transplantation, but these are generally performed after the immediate post-transplant period and to our knowledge there are no studies examining the initial inflammatory phase during and/or immediately following islet cell infusion. The purpose of this study was to compare the serum cytokine profiles following allogenic and autologous islet cell transplantation in the early post-operative phase and to examine them in relation to the mode of induction therapy. We present the following article in accordance with the MDAR reporting checklist (available at http://dx.doi.org/10.21037/atm-20-3519).

Methods

Patients

Serum samples from patients who had received an islet transplant were obtained from three international centres; autologous islets following total pancreatectomy and digestion of the pancreas from the Leicester General Hospital (LGH; Leicester, United Kingdom), the Baylor Research Institute (BRI; Dallas, United States) and allogenic islets from the BRI and the Royal Adelaide Hospital (RAH; Adelaide, Australia and New Zealand Clinical Trials Registry No 083020). Two control patients were also recruited who had undergone total pancreatectomy without islet transplantation. The study was conducted in accordance with the Declaration of Helsinki (as revised in 2013). The study was approved by the institutional review board of University Hospitals of Leicester NHS Trust (EDGE-34223), and written informed consent was obtained from all patients included in the study.

Autologous islet protocol

In the Leicester series, islets were prepared and infused as previous described (25). The resected pancreas was digested with the neutral protease NB GMP Grade in combination with purified Collagenase NB 1 GMP Grade (SERVA Electrophoresis GmbH, Heidelberg, Germany). Unpurified whole pancreatic digest was suspended in a 20% human serum albumin and M199 transplant media. Islets were prepared while the surgeons completed the surgical reconstruction (26). Immediately prior to the islet infusion the patients received 5000 iU heparin intravenously. Islets were infused into the portal vein over 20–30 minutes via a recannalised umbilical vein and during the islet infusion the portal pressure were continuously monitored to ensure that the it remained below 20 mmHg (27).

In the BRI patients islets were prepared and infused again as previous described (28). Pancreata were digested with either Liberase MTF with Thermolysin (Roche, Indianapolis - United States) or Collagenase NB 1 GMP Grade (SERVA Electrophoresis GmbH, Heidelberg, Germany). Isolated islets were infused into the portal vein via a mesenteric vein with added heparin (70 iU/kg body weight) over 30 to 60 minutes while the patients were under general anaesthesia. During islet infusion, portal vein pressure was monitored intermittently. If the portal vein pressure exceeded 20 mmHg, the infusion of islets was discontinued until portal vein pressure decreased.

Allogenic islet transplant

In the BRI series islets were prepared and evaluated as previously described (29). Briefly, pancreatic ductal injection was performed with the ET-Kyoto solution and the oxygen-charged static two-layer method (29,30). The pancreas was infused with collagenase NBI and neutral protease (SERVA Electrophoresis, Germany). Islets were purified by means of a continuous density gradient in a COBE 2991 cell processor with Ficoll solution, and were assessed using dithizone staining to determine the yield and purity. The islet yield was then converted into a standard number of islet equivalents. Islet viability was measured with fluorescein diacetate and propidium iodide staining.

Rabbit anti-thymocyte globulin (ATG, Thymoglobulin, Genzyme, MA) was administered intravenously at a dose of 1.5 mg/kg on days 0, 2, 4 and 6 post-transplant for the induction of immunosuppression. Tacrolimus (Prograf, Astellas Inc) and mycophenolate mofetil (MMF, CellCept, Roche Laboratories, NJ) (2 g/day orally) were used to maintain immunosuppression. Anakinra (Kineret, Amgen, CA) (100 mg) was administered intravenously an hour pre-transplant and subcutaneously for 7 days following transplantation. In addition, etanercept (Enbrel, Immunex, WA) (50 mg) was administered intravenously an hour pre-transplant and subcutaneously on days 3, 6 and 10 post-transplantation.

In the series from Adelaide (Australia and New Zealand Clinical Trials Registry) islets were separated at two islet isolation facilities as described previously using a variation of the closed loop method described by Ricordi et al. (31,32). Donor pancreata were removed from heart-beating deceased donors and ductal infusion was performed with cold collagenase NB1 GMP grade (SERVA, Heidelberg, Germany) or Liberase HI GMP grade (Roche Indianapolis, IN). Dissociated islet and acinar tissue were separated on a continuous Biocoll (Biochrom AG, Berlin) density gradient (polysucrose 400 and amidotrizoic acid) on a refrigerated apheresis system (Model 2991, COBE Laboratories, Lakewood, Colorado).

All patients received tacrolimus (Astellas Inc.), mycophenolate mofetil (Roche) and rabbit anti-thymocyte globulin (Fresenius). ATG was administered intravenously at a dose of 3 mg/kg for 5 days at the time of transplant. Tacrolimus (Prograf, Astellas Inc.) was commenced at a dose of 0.1 mg/kg per day in two equal divided doses, with the dose adjusted to achieve a target blood concentration of 10 ng/mL. MMF was commenced at a dose of 1gm bd.

Serum tests

Serum samples were obtained during induction of the anaesthesia, at 15 and 30 minutes prior to islet infusion, at the end of the operation, and subsequently at 1, 3, 6, 24 hours and 2, 3, 5, and 7 days post islet infusion. Serum samples were stored at –80 °C until assayed centrally in Leicester.

Secretion of cytokines and chemokines was determined by measuring serum samples in a Luminex 200 (Millipore, Billerca, Massachussetts, USA) using xMAP technology. The bead assay was performed according to the manufacturer’s instructions with a sensitivity of 1 pg/ml/cytokine. Markers were interferon alpha (IFN-α), interferon gamma (IFN-γ), interleukin-10 (IL-10), interleukin-12p40 (IL-12p40), interleukin-12p70 (IL-12p70), interleukin-13 (IL-13), interleukin-15 (IL-15), interleukin-17 (IL-17), interleukin-1ra (IL-1ra), interleukin-1 alpha (IL-1α), interleukin-1 beta (IL-1β), interleukin-2 (IL-2), interleukin-3 (IL-3), interleukin-4 (IL-4), interleukin-5 (IL-5), interleukin-6 (IL-6), interleukin-7 (IL-7), interleukin-8 (IL-8), Epidermal growth factor (EGF), Eotaxin, Granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF), vascular endothelial growth factor (VEGF), Monocyte Chemotactic Protein-1 (MCP-1), macrophage inflammatory protein 1 alpha (MIP-1α), macrophage inflammatory protein 1 beta (MIP-1β), tumour necrosis factors-alpha (TNF-α) and tumour necrosis factors-beta (TNF-β). The Wieslab® Complement system Screen (Euro-Diagnostica, Malmö, Sweden) was used according to the manufacturer’s instructions.

Statistical analysis

All data were initially entered into an Excel database (Microsoft, Redmond, Washington, USA) and the analysis was performed using the Statistical Package for the Social Sciences Windows, version 13.0 (SPSS, Chicago, Illinois, USA). Descriptive statistics consisted of the mean and standard deviation for parametric distributions and median and range for non-parametric distributions after confirmation with the Kolmogorov-Smirnov test and histograms.

Comparisons over time were performed with the Friedman test for non-parametric variables. Comparison among the patient cohorts were performed using Mann-Whitney test for non-parametric variables. A “P” value of less than 0.05 was considered statistically significant.

Results

Eleven autologous and six allogenic islet cell transplant patients participated in the study from 2010-2012 and the demographics are shown in . Pre-transplant haemoglobin A1c (HbA1c) levels were significantly higher in the islet allogenic cohort than in the autologous group (P=0.05) (). Transplanted islet cell suspension volumes were significantly lower in the allogenic islet cell transplant group than in the autologous islet cell transplantation group (P<0.05) (). Islet purity was significantly higher in the allogenic than in the autologous group (P<0.05) ().

Table 1

Patient and islet characteristics of the islet auto-transplant group

Patient	Age (yr)	BMI (kg/m2)	Total IEQ	IEQ/kg	Tissue volume (mL)	Cleavage/Purity (%)	Viability (%)	Outcomes (1 year)	HBA1c (%)
Pt 1	56	24	645,066	10,523	24	67	99	Insulin dependent	5.2
Pt 2	38	20	369,177	6,616	31	67	95	Insulin dependent	5.9
Pt 3	46	25.39	292,866	3,854	23	36	97	Insulin free	5.1
Pt 4	54	19.2	209,818	3,568	10	40	96.8	Insulin dependent	5.5
Pt 5	48	27.1	1,010,127	13,669	18	53	96.1	Insulin dependent	5.5
Pt 6	41	19.7	363,938	6,972	10	60	94.3	Insulin free	4.9
Pt 7	26	21.8	168,263	3,319	9	50	95.5	Insulin dependent	8
Pt 8	40	29	507,664	6,418	9.5	50	97.4	Insulin free	5.5
Pt 9	54	28.4	331,800	4,628	9.5	20	94.4	Insulin dependent	6.6
Pt 10	44	30	375,417	4,995	0.8	45	96.8	Insulin dependent	6.1
Pt 11	35	40.4	500,351	4,284	10	20	95.5	Insulin dependent	6

Table 2

Patient and islet characteristics of the islet allo-transplant group

Patient	Age (yr)	BMI (kg/m2)	Total IEQ	IEQ/kg	Tissue volume (mL)	Cleavage/Purity (%)	Viability (%)	Outcomes (1 year)	HBA1c (%)
Pt 1	35	21.2	650,942	12,010	9	61	94.3	Insulin free	6
Pt 2	46	22.2	828,050	10,442	9.5	61	96.1	Insulin free	6.8
Pt 3	41	26	371,238	5,156	1.25	80	74	Insulin dependent	7.4
Pt 4	46	21.5	494,670	7,978	1.6	86	91	Insulin dependent	9.4
Pt 5	46	21.5	350,000	5,833	1.5	80	88	Insulin free	9.4
Pt 6	48	23.8	340,000	5,480	1.55	95	82	Insulin dependent	13.8

Table 3

Patient and islet characteristics of the islet auto- and allo-transplant groups

	Islet auto-transplantation	Islet allo-transplantation	P value
Age (years)	43.8±9.1	43.7±4.8	ns
BMI	25.9±6.2	22.7±1.9	ns
Pretransplant HbA1c (%)	5.8±0.9	8.8±2.8	0.05
Total transplanted islets (IEQ)	434,044±234,537	505,817±197,436	ns
Transplanted islets (IEQ/kg)	6,259±3,218	7,817±2,813	ns
Tissue volume (mL)	14.1±8.8	4.1±4.0	<0.05
Purity (%)	46.2±16.3	77.2±13.7	<0.05
Viability (%)	96.2±1.4	87.6±8.3	ns

The data of Age, BMI, Pretransplant HbA1c, Total transplanted islets, Transplanted islets/kg, Tissue volume, purity and viability were expressed as mean ± SD.

The cytokine changes that occurred in patients who underwent total pancreatectomy followed by autologous islet transplantation were not significantly different from the changes observed in the control group of patients who only underwent a total pancreatectomy. The Initial increase in cytokine levels occurred during induction of anaesthesia and during the surgical resection ().

Figure 1

Cytokines comparison. (A) The comparison between total pancreatectomy and islet auto transplant patients. Comparison of TNF α, IL-10, IL-8 GM-CSF. (B) The comparison between islet auto and allotransplant patients. Comparison of TNF α, MIP-1α, IP-10, MIP-1β, MCP-1, IL-8, IL-1α, IL-10, G-CSF, IL-1ra, GM-CSF, Eotaxin. *, P<0.05.

Cytokines changes

Significantly elevated cytokine concentrations following allogenic transplantation which compared with recipients of autologous transplants included; TNF-α (P<0.001), MIP-1α (P=0.001), IP-10 (P=0.001), MIP-1β (P=0.0002), MCP-1 (P=0.013), IL-8 (P=0.004), IL-1α (P=0.001), IL-10 (P=0.001), G-CSF (P=0.0198), IL-1ra (P=0018), GM-CSF (P=0.001) and Eotaxin (P=0.001) (). IL-6 concentrations were initially higher in the allogenic group compared with the autologous group, but after the first hour post infusion this finding was reversed with higher concentrations in the autologous group (P<0.0001). In autologous recipients EGF concentrations at completion of the infusion, 15 minutes following infusion and on the first postoperative day were all significant lower compared with pre-infusion levels.

Cytokines differences in allogenic versus autologous transplantation group over time ()

Lower initial concentrations of the following cytokines were noted in the patients receiving autologous transplants compared with patients who received allografts; IFN-α (P<0.001), IFN-γ (P<0.001) IL-12p40 (P<0.0001), IL-12p70 (P<0.0001) and IL-17 (P<0.0001), but these differences were not sustained. Higher concentrations of IL-13 (P<0.0001) and IL-4 (P<0.001) were observed following autologous compared with allogenic islet cell transplantation. Eotaxin A concentrations were increased significantly compared with pre-infusion levels (P<0.0001) until the 6th postoperative day. After day 6 levels returned to those found preoperatively. IL-15 concentrations on the first and third postoperative days were significantly higher than on admission (P<0.05). IL-1β concentrations were higher compared to baseline levels from the third to seventh postoperative day in the allogenic transplant group and findings were similar for IL-5 and VEGF concentrations in the autologous group and for concentrations of IL-7 concentrations in both groups.

Complement activity

Mannose-binding lectin (MBL) functional pathway deficiency was found in four patients following autologous islet cell infusion and one patient following an allogenic transplant. Conversely classic pathway (CP) activity was reduced compared with baseline levels from the completion of the islet infusion to the 3rd postoperative day, when it recovered to preoperative levels (). These changes were also seen in both the MBL and alternative pathway (AP) activities in both groups (). C3 and C4 complement components reduced in the acute phase and were similar in both groups of patients ().

Figure 2

Complement activity over time among islet auto and allotransplant patients. (A) Classical pathway activity; (B) MBL pathway activity. Patients with no MBL pathway activity have been removed; (C) alternative pathway activity; (D) C3 level; (E) C4 level. MBL, mannose-binding lectin.

Correlations with insulin-free or insulin-dependent outcome

Eight patients were insulin dependent and three patients insulin free following autologous islet cell transplantation compared with three patients who were insulin dependent and three patients were insulin free following allogenic transplantation. There were no statistically significant differences in ∆VEGF and ∆IL-10 levels between the two groups. However, ∆VEGF levels were higher in the insulin free group when compared with the insulin dependent group in allogenic patients (227.7±228.6 pg.mL−1 vs. 30±41.5 pg.mL−1). ∆IL-10 levels were six-fold higher in the insulin-free group compared with in the insulin-dependent group in the allogenic patients (1,389.3±1,080.6 vs. 213.3±178.2 pg.mL−1) ( and ).

Table 5

∆IL10 and ∆VEGF differences between the insulin free and insulin dependent groups in patients receiving islet allo-transplants

	Islet allo-transplantation
	Insulin free (n=3)	Insulin dependent (n=3)
∆VEGF (pg/mL)	227.7±228.6	−30±41.5
∆IL-10 (pg/mL)	1389.3±1080.6	213.3±178.2

The data ∆VEGF and ∆IL-10 were expressed as mean ± SD. ∆ changes before and 30 minutes after islet infusion. VEGF, vascular endothelial growth factor.

Figure 3

Differences between the insulin-dependent group and insulin-free group in islet allo- and auto-transplant patients. (A) The changes in IL-10 between admission and peak levels in islet allotransplant patients and (B) the changes in VEGF between admission and first peak levels in islet auto-transplant patients. (C) The baseline MBL functional activity in islet allo- and auto-transplant patients. VEGF, vascular endothelial growth factor; MBL, mannose-binding lectin.

Although MBL functional activity in patients that were insulin free was half that of the insulin dependent group (0.31±0.59 vs. 0.63±0.45) this was not statistically significant ().

Differences in cytokine profiles across institutions

A comparison of the serum cytokine responses between allogenic islet recipients treated with two different induction regimens () was performed to examine the influence of these drugs in the early inflammatory phase. In the two patients from the USA the regimen included interleukin-1 receptor antagonism (IL1ra; Anakinra) and anti-TNF-α (Etanercept) in combination with T cell depletion with anti-thymocyte globulin. This contrasted with our Australian patient cohort who were treated with anti-thymocyte globulin alone. Both groups received maintenance with CNI and tacrolimus. The Australian cohort had elevated levels of IL-8, TNF-α, MCP-1 and Eotaxin compared with the IL1ra/TNF-α treated patients ().

Figure 4

Baylor and Adelaide immunosuppression protocols.

Figure 5

Differences in cytokine profiles in Allotransplant patients treated with two different regimes. (A) IL-8; (B) TNF-alpha; (C) MCP-1; (D) eotaxin.

Discussion

The long-term outcome of islet transplantation is critically dependent on the number of viable islets that survive the immediate post-transplant period, implant and continue to function in the long-term. Many factors contribute to poor islet viability including pre-transplant factors, the toxicity of different immunosuppressive regimens (33-36) and activation of the coagulation cascade (37).

The comparison of autologous islet transplantation with allogenic islet transplantation provides a unique insight into the systemic innate immune response in the absence of human leukocyte antigen (HLA) mismatches and immunosuppression. We postulate that the improved functional advantage of autografts is due to the absence of rejection conferred by autoimmunity. Examining the systemic inflammatory response to human islet tissue in vivo may identify factors that could be targets for therapeutic peri-transplant immunosuppressive regimens (7,38).

Islet cell transplantation, whether allografts or autotransplants, initiates a generalised inflammatory response, manifesting as elevated biochemical markers, raised leukocyte counts and elevated acute phase proteins. Inflammatory mediators including cytokines, chemokines, and complement activation products may well contribute to the early loss of islets (7).

This aim of this study was to investigate the release of those cytokines, chemokines and activated complement components that occurs immediately following islet cell infusion. The different inflammatory patterns and the degree of systemic involvement in both forms of islet cell transplantation were varied and supported the hypothesis that the early phase following transplantation represents a crucial period for the infused islets. In patients receiving an allograft, markedly raised levels of EGF, GM-CSF, IL-1ra, IL-1α, IL-2, IL-8, IL-10, MIP-1β and TNF-α are found which contrasts sharply with the findings in the autologous islet cell recipients. The primary cytokines released after islet cell transplantation play an important role during the acute-phase response (35). Several studies have shown that combinations of cytokines such as IL-1β, IFN-γ and TNF-α lead to islet cell death or dysfunction (39). In our study, TNF-α is significantly elevated in patients following allogenic islet cell infusion compared with those receiving an autologous transplant. In patients receiving the Baylor protocol, which utilises TNF-α inhibitors, it has been shown that TNF-α levels are significant mediators of processes involved in islet cell dysfunction or death (40). The Baylor group has also demonstrated that a dual combination regimen of IL-1β and TNF-α inhibition reduced islet damage resulting from non-specific inflammation (41,42).

IL-8, classified as a CXC-chemokine (the two N-terminal cysteines of CXC chemokines are separated by one amino acid, represented by “X”), induces chemotaxis and the activation of T cells and granulocytes. The persistent elevation of these chemokines during islet cell transplantation may potentially reflect the involvement of granulocytes causing early loss of cell mass (43). In contrast to the rise of several inflammatory cytokines and changes in the anti-inflammatory cytokine IL-10 were modest, further supporting a net inflammatory response during islet transplantation. IL-10 may however play an important role in the immune regulation and in our study IL-10 levels were significantly raised in allogenic islet cell transplant patients compared with those receiving an autologous transplant (44). Furthermore, IL-10 levels were found to be six-fold higher in patients who became insulin independent compared with those who were insulin dependent. This is in agreement with Huurman et al. who investigated the role of IL-10 in preventing the onset of diabetes in non-diabetic mice following islet cell allo-transplantation (45,46).

Long-term insulin independence following islet transplantation depends on an adequate islet cell mass (islet equivalents) and early and complete revascularization of islet cell clusters to facilitate engraftment and subsequent survival and function. Delayed or incomplete revascularization may cause early islet loss and graft failure and previous studies have reported that levels of VEGF, as a surrogate for revascularisation, were markedly reduced in the first few days post-operatively and that it may take two weeks for transplanted islets to undergo complete and stable neo-vascularization (47,48). In our study, we demonstrated a similar pattern with VEGF levels being reduced for 72 hours following islet transplantation, but increasing again after day 4. Interestingly, we also found that VEGF produced two peaks in the insulin free cohort following allogenic transplantation with the first peak observed 30 minutes after transplantation.

Complement is another important component of the response to islet transplantation and a determinant of the success or failure of engraftment particularly in the initial phase. It is part of the instant blood-mediated inflammatory reaction (IBMIR) which occurs at the time of intra-portal infusion and involves the coagulation and complement cascades. Complement activation is well described in reperfusion injury during organ transplantation and we have previously studied complement activation and deficiency in islet transplantation (49-51).

It is generally believed that complement activation is a short, transient innate immune response (49) and although our study has confirmed that complement activation does occur immediately following surgery we have also shown that activation can continue for 72 hours. Complement activation increases C3a and C5a which initiates triggers of the pro-inflammatory response and is a likely candidate involved in islet cell loss in the postoperative period (52). MBL functional activity was however low in the insulin-free cohorts in both groups and these preliminary results suggest that deficiency of the MBL pathway may result in a better clinical outcome following islet cell transplantation. This is supported by our previous studies where we found that MBL deficiency or reduced activity improved the outcome in autologous transplantation patients (51).

This study also provides novel data regarding the cytokine profile of islet allograft recipients treated with two different induction regimes. Although the patient numbers were small, there were marked differences between the induction regimes with in vivo human data demonstrating a marked reduction in a range of inflammatory mediators. Studies from the Collaborative Islet Transplant Registry (CITR) suggest that addition of T cell depletion and anti-TNF-α significantly improve the rate of insulin independence following islet transplantation. The present data provides further supporting evidence for the reduction of inflammation by the addition of agents producing IL1ra and TNF-α antagonism.

Conclusions

Allogenic islet cell transplantation produces a significantly raised inflammatory profile in comparison with autologous islet cell transplantation in the initial post-infusion phase. There remains a need to fully understand all the contributory components and to develop strategies which will abrogate the response and maximise islet cell engraftment, survival and long-term function.

The article’s supplementary files as

Table 4

Schematic summary of cytokine modifications

	Differences between groups
	No	Yes
Differences over time
No	IL-3, TNF-β	IFN-α, IFN-γ, IL-12p40, IL-12p70, IL-17, IL-13, IL-4
Yes	Eotaxin A, IL-15, IL-1β, IL-5, VEGF, IL-7, EGF, IL-6	TNF-α, MIP-1α, IP-10, MIP-1β, MCP-1, IL-8, IL-1α, IL-10, G-CSF, IL-1ra, GM-CSF, Eotaxin