Literature DB >> 33563229

Linkage analysis, GWAS, transcriptome analysis to identify candidate genes for rice seedlings in response to high temperature stress.

Zhaoran Wei1,2, Qiaoling Yuan1, Hai Lin1, Xiaoxia Li1, Chao Zhang1, Hongsheng Gao1, Bin Zhang1, Huiying He1, Tianjiao Liu1, Zhang Jie1, Xu Gao1, Shandang Shi1, Bo Wang1, Zhenyu Gao3, Lingrang Kong2, Qian Qian4,5, Lianguang Shang6.   

Abstract

BACKGROUND: Rice plants suffer from the rising temperature which is becoming more and more prominent. Mining heat-resistant genes and applying them to rice breeding is a feasible and effective way to solve the problem. RESULT: Three main biomass traits, including shoot length, dry weight, and fresh weight, changed after abnormally high-temperature treatment in the rice seedling stage of a recombinant inbred lines and the natural indica germplasm population. Based on a comparison of the results of linkage analysis and genome-wide association analysis, two loci with lengths of 57 kb and 69 kb in qDW7 and qFW6, respectively, were associated with the rice response to abnormally high temperatures at the seedling stage. Meanwhile, based on integrated transcriptome analysis, some genes are considered as important candidate genes. Combining with known genes and analysis of homologous genes, it was found that there are eight genes in candidate intervals that need to be focused on in subsequent research.
CONCLUSIONS: The results indicated several relevant loci, which would help researchers to further discover beneficial heat-resistant genes that can be applied to rice heat-resistant breeding.

Entities:  

Keywords:  GWAS; High-temperature-mediated growth response; Linkage analysis; Rice seedling; Transcriptome analysis

Mesh:

Year:  2021        PMID: 33563229      PMCID: PMC7874481          DOI: 10.1186/s12870-021-02857-2

Source DB:  PubMed          Journal:  BMC Plant Biol        ISSN: 1471-2229            Impact factor:   4.215


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