Maria Catalina Ospina-Pinto1, Patricia Hernández-Rodríguez2. 1. School of Agricultural Sciences. Molecular Biology and Immunogenetics Research Group (BIOMIGEN), Universidad de La Salle, Bogotá, Colombia. 2. Department of Basics Sciences. Molecular Biology and Immunogenetics Research Group (BIOMIGEN), Universidad de La Salle, Carrera 2 No. 10 - 70, Bogotá, Colombia. phernandez@unisalle.edu.co.
Abstract
INTRODUCTION: Leptospirosis, caused by Leptospira spp., is a zoonosis with multiple hosts and reservoirs. Thus, the "One Health" approach must be addressed in order to guide prevention and control measures regarding this microorganism. There are few studies involving all stages of the pig production cycle and potential sources of infection such as water sources, which could represent a risk for local rural communities. OBJECTIVE: To identify Leptospira spp. in the animal-environment interface (swine-water) in the pig production cycle. MATERIALS AND METHODS: The study was conducted in a swine farm in Puerto López in the department of Meta, Colombia. Swine blood samples (n = 65) were collected for microscopic agglutination test (MAT), while urine and water samples (n = 80) were tested for Leptospira using bacterial isolation, serotyping of the isolates, and polymerase chain reaction (PCR). RESULTS: A total of 58 (89.2%) porcine sera were positive for at least one Leptospira serogroup, with Grippotyphosa as the most frequent serogroup. Leptospira spp. were isolated in 25% (20/80) of the urine and water samples. Leptospira-positive cultures were 100% (20/20) identified within the genus Leptospira (rrl gene) and 50% (10/20) corresponded to pathogenic species (lipL32 gene) by PCR, and 45% (9/20) were positive to at least one antiserum. None of the direct urine and water samples was positive by PCR. CONCLUSION: It is suggested that the bacteria might be transmitted in the animal-environment interface in the pig production cycle.
INTRODUCTION:Leptospirosis, caused by Leptospira spp., is a zoonosis with multiple hosts and reservoirs. Thus, the "One Health" approach must be addressed in order to guide prevention and control measures regarding this microorganism. There are few studies involving all stages of the pig production cycle and potential sources of infection such as water sources, which could represent a risk for local rural communities. OBJECTIVE: To identify Leptospira spp. in the animal-environment interface (swine-water) in the pig production cycle. MATERIALS AND METHODS: The study was conducted in a swine farm in Puerto López in the department of Meta, Colombia. Swine blood samples (n = 65) were collected for microscopic agglutination test (MAT), while urine and water samples (n = 80) were tested for Leptospira using bacterial isolation, serotyping of the isolates, and polymerase chain reaction (PCR). RESULTS: A total of 58 (89.2%) porcine sera were positive for at least one Leptospira serogroup, with Grippotyphosa as the most frequent serogroup. Leptospira spp. were isolated in 25% (20/80) of the urine and water samples. Leptospira-positive cultures were 100% (20/20) identified within the genus Leptospira (rrl gene) and 50% (10/20) corresponded to pathogenic species (lipL32 gene) by PCR, and 45% (9/20) were positive to at least one antiserum. None of the direct urine and water samples was positive by PCR. CONCLUSION: It is suggested that the bacteria might be transmitted in the animal-environment interface in the pig production cycle.
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