Avelumab, a PD-L1 Inhibitor, in Combination with Hypofractionated Radiotherapy and the Abscopal Effect in Relapsed Refractory Multiple Myeloma.

LESSONS LEARNED: Despite the initial optimism for using immune checkpoint inhibition in the treatment of multiple myeloma, subsequent clinical studies have been disappointing. Preclinical studies have suggested that priming the immune system with various modalities in addition to checkpoint inhibition may overcome the relative T-cell exhaustion or senescence; however, in this small data set, radiotherapy with checkpoint inhibition did not appear to activate the antitumor immune response.
BACKGROUND: Extramedullary disease (EMD) is recognized as an aggressive subentity of multiple myeloma (MM) with a need for novel therapeutic approaches. We therefore designed a proof-of-principle pilot study to evaluate the synergy between the combination of the anti-PD-L1, avelumab, and concomitant hypofractionated radiotherapy.
METHODS: This was a single-arm phase II Simon two-stage single center study that was prematurely terminated because of the COVID-19 pandemic after enrolling four patients. Key eligibility included patients with relapsed/refractory multiple myeloma (RRMM) who had exhausted or were not candidates for standard therapy and had at least one lesion amenable to radiotherapy. Patients received avelumab until progression or intolerable toxicity and hypofractionated radiotherapy to a focal lesion in cycle 2. Radiotherapy was delayed until cycle 2 to allow the avelumab to reach a study state, given the important observation from previous studies that concomitant therapy is needed for the abscopal effect.
RESULTS: At a median potential follow-up of 10.5 months, there were no objective responses, one minimal response, and two stable disease as best response. The median progression-free survival (PFS) was 5.3 months (95% confidence interval [CI]: 2.5-7.1 months), and no deaths occurred. There were no grade ≥3 and five grade 1-2 treatment-related adverse events.
CONCLUSION: Avelumab in combination with radiotherapy for patients with RRMM and EMD was associated with very modest systemic clinical benefit; however, patients did benefit as usual from local radiotherapy. Furthermore, the combination was very well tolerated compared with historical RRMM treatment regimens. © Published 2021. This article is a U.S. Government work and is in the public domain in the USA.

Discussion

Immunotherapy with PD‐1/L1 checkpoint inhibition has led to a revolution in the treatment of various malignancies—directly translating to longer patient survival. However, to date, PD‐1/L1 inhibition in the treatment of MM has not shown similar success. We hypothesized that radiotherapy might synergize with PD‐1/L1 inhibition and lead to more significant tumor responses via an abscopal effect. However, in this small study (Fig. 1), antitumor responses with the combination appeared modest. At the data cutoff date of November 1, 2020, all four enrolled patients had discontinued therapy because of progression. Three patients received 5 Gy of radiation to the EMD site for 5 days, and one patient received 2 Gy for 5 days because of a lesion on the skull. At a median potential follow‐up of 10.5 months, the overall response rate (ORR) was 0. One patient had a minimal response, and two patients had stable disease as their best response, resulting in a clinical benefit rate of 75.0% (95% CI: 19.4%–99.4%). The median PFS was 5.3 months (95% CI: 2.5–7.1 months) with a 6‐month PFS rate of 50% (95% CI: 5.8%–84.4%). At the time of this analysis, all patients were alive. Treatment was well tolerated with no grade ≥3 treatment‐related adverse events.

A major limitation of this study is the small sample size, and the question remains whether we would observe deep responses in a small subset of patients if we had enrolled more patients. Altogether, given our findings and those of previous studies, anti–PD‐1/L1 combination regimens (with immunomodulatory drugs or radiotherapy) do not appear to be synergistic in the clinical setting. However, one major issue still remains, namely, the ideal radiotherapy strategy in terms of not only dose, fractionation, timing, and duration, but also of whether multiple sites of radiation are needed to adequately prime the immune system and induce tumor‐associated antigens. Therefore, it is unknown whether, in the near future, PD‐1/L1 inhibitors will have a role in the treatment of MM.

Trial Information

Drug Information

Patient Characteristics

Primary Assessment Method

Adverse Events

Assessment, Analysis, and Discussion

Multiple myeloma (MM) remains incurable, with a median survival of 5–8 years [1]. Because of the recent therapeutic advances and development of highly efficacious MM regimens, patients more frequently develop extramedullary disease (EMD) during the longer disease course. EMD, now recognized as an aggressive subentity of MM, is characterized by the ability of a MM subclone to proliferate independently of the bone marrow microenvironment either by direct invasion from the medullary compartment disrupting the cortical bone or less commonly by hematogenous metastatic spread [2, 3]. Approximately 15% of patients newly diagnosed with MM present with EMD, which is associated with shorter progression‐free survival (PFS) and overall survival (OS) [4, 5]. Therefore, novel approaches to treatment are needed for this growing population of patients.

Pathways that inhibit antitumor T‐cell responses include the activation of the inhibitory programmed cell death 1 (PD‐1) and PD‐ligand 1 (PD‐L1) axis, allowing tumors to evade the immune system. Despite the success of monotherapy PD‐1/L1 inhibitors in solid tumors and promising preclinical results, antitumor activity in MM has been modest [6, 7, 8, 9]. Preclinical studies have shown that the combination of radiotherapy and PD‐1/L1 blockade may enhance antitumor activity by increasing interferon gamma, tumor antigen cross presentation, T‐cell receptor clonality, and PD‐L1 expression and reinvigorating tumor infiltrating lymphocytes while decreasing immunosuppressive myeloid derived suppressor and regulatory T cells [10]. Synergy between radiation and PD‐1 pathway inhibitors has been observed in breast, colon, melanoma, and glioma tumor models [11, 12, 13, 14]. We hypothesized that targeted radiation to a site of EMD may change the MM microenvironment niche to sensitize myeloma cells to PD‐1/L1 inhibition and activate systemic antitumor immune responses. We designed a proof‐of‐principle pilot clinical study to evaluate the combination of avelumab, an anti–PD‐L1 IgG1 monoclonal antibody, approved by the U.S. Food and Drug Administration for the treatment of Merkel cell, urothelial, and renal cell carcinomas, with concomitant hypofractionated radiotherapy.

Patients were enrolled in this single‐arm phase II single center study (NCT03910439) between April 10, 2019, and November 1, 2020. The study was prematurely terminated after enrolling four patients because of poor accrual in the face of the COVID‐19 pandemic. The study was approved by the National Cancer Institute Institutional Review Board, and patients provided written informed consent. Patients had documented relapsed/refractory MM and (a) had progressed on two or more prior lines of therapy and (b) had exhausted, or were not candidates for, additional MM therapy. Other key eligibility criteria included having (c) at least one extramedullary or lytic lesion deemed a candidate for radiotherapy by a radiation oncologist, (d) measurable disease, and (e) adequate organ function. Patients received a fixed dose of avelumab 800 mg i.v. on days 1 and 15 of every cycle (28‐day cycles) until progression or intolerable toxicity (Fig. 1). Additionally, patients received focal hypofractionated radiation therapy on cycle 2 days 1–5 at a goal strategy of 5 Gy daily for 5 days (adjusted at the radiation oncologist's discretion). The primary endpoint was to determine the overall response rate (ORR) according to the International Myeloma Working Group Response Criteria. Secondary endpoints included determination of the complete response rate, PFS, OS, and tolerability captured by National Cancer Institute Common Terminology for Adverse Events. Serum protein electrophoresis and immunofixation were assessed at baseline and at the start of every cycle.

Figure 1

Avelumab in combination with radiation therapy in relapsed refractory multiple myeloma. (A): Study design. (B): Eligibility, endpoints, and statistical plan. Abbreviations: CR, complete response; FDG PET, fluorodeoxyglucose–positron emission tomography; IMiD, immunomodulatory drug; IV, intravenous; MRDnegCR, minimal residual disease negative complete response; ORR, overall response rate; OS, overall survival; PFS, progression‐free survival; PI, proteosome inhibitor; RRMM, relapsed/refractory multiple myeloma; XRT, radiation therapy.

Waterfall plot of best percent change in M‐protein values and IMWG response. Abbreviation: IMWG, International Myeloma Working Group.

At the data cutoff date of November 1, 2020, all four patients had discontinued therapy because of progression. All patients received avelumab as scheduled. Three patients received 5 Gy of radiation to the EMD site for 5 days, and one patient received 2 Gy for 5 days because of a lesion on the skull. At a median potential follow‐up of 10.5 months, the ORR was 0. One patient had a minimal response, and two patients had stable disease as their best response, resulting in a clinical benefit rate of 75.0% (95% confidence interval [CI]: 19.4%–99.4%). The median PFS was 5.3 months (95% CI: 2.5–7.1 months) with a 6‐month PFS rate of 50% (95% CI: 5.8%–84.4%). At the time of data cutoff, all patients were alive. Treatment was well tolerated with no grade ≥3 treatment‐related adverse events. In total, five grade 1–2 treatment‐related adverse events occurred in two of four patients and included alanine/aspartate aminotransferase elevation, arthralgia, general pain, and maculopapular rash.

Immunotherapy with PD‐1/L1 checkpoint inhibition has led to a revolution in the treatment of various malignancies—directly translating to longer patient survival. However, to date, PD‐1/L1 inhibition in the treatment of MM has not shown similar success. We hypothesized that radiotherapy might synergize with PD‐1/L1 inhibition and lead to more significant tumor responses via an abscopal effect. However, in this small study, antitumor responses with the combination appeared modest. No patients attained a partial response or better, and the median PFS was 5.3 months.

Recently, the abscopal effect and the use of radiotherapy as a combination partner “drug” has been of great interest with mixed conclusions, and more than 200 studies have been initiated [15]. Since the concept and implementation of our study, further results have been published regarding the abscopal effect, notably in solid cancers, as a potential mechanism of augmenting immune checkpoint inhibitor efficacy results. Interestingly, out of the vast number of studies, fewer than 10 studies have been noted to allow for delivery of radiotherapy to >1 lesion. As Brooks and Chang discuss, one focal site might not be sufficient to induce tumor‐associated antigens resulting in modest or no improvements observed with abscopal studies to date [15]. Response rates in radiotherapy combination arms have been similar to checkpoint inhibitor monotherapy arms. For example, McBride et al. compared anti–PD‐1 therapy (nivolumab) with and without stereotactic body radiotherapy in a randomized head and neck cancer study, but the combination did not improve outcomes [16]. Therefore, moving forward, perhaps a wiser strategy will be to irradiate as many lesions as possible in combination studies rather than a single focal site. Therefore, outside of a clinical trial, combination therapy to induce the abscopal effect should not be pursued unless patients have a clear indication (symptomatic or progressive lesions) for palliative radiotherapy [17]. Although our study allowed for irradiation of multiple sites as clinically indicated, the small sample size does not allow us to make any conclusions with our combination and single versus multiple sites of irradiation. In terms of relapsed/refractory MM specifically, irradiating all sites may be problematic, as patients with a heavy EMD burden may not be able to wait long enough for delayed immunotherapy responses. Furthermore, decreased bone marrow function and cytopenias from both MM and prior therapies may be problematic in this patient population and only exacerbated by multiple sites of irradiation.

A major limitation of this study is the small sample size, and the question remains whether we would observe deep responses in a small subset of patients if we had enrolled more patients. Given our and others’ findings, it is unknown whether immune checkpoint inhibitor combination regimens will synergize to produce meaningful clinical benefit. Therefore, excluding clinical trials, PD‐1/L1 inhibitors are unlikely to have a role in the MM clinic in the near future.

Disclosures

The authors indicated no financial relationships.

Disease	Multiple myeloma
Stage of Disease/Treatment	Metastatic/advanced
Prior Therapy	More than two prior regimens
Type of Study	Phase II, single arm
Primary Endpoint	Overall response rate
Secondary Endpoints	Complete response rate, progression‐free survival, overall survival, tolerability
Additional Details of Endpoints or Study Design	The statistical design incorporated a two‐stage Simon minimax design where the first stage would enroll 13 patients, and if one response occurred, the study would proceed to the second stage for a total enrollment of 27 patients. The study would be considered a success if four total responses occurred (14.7%), with an alpha of 5% and a power of 80%.
	Response Definition: The 2014 International Myeloma Working Group (IMWG) response criteria for multiple myeloma was used. In brief, response criteria are as follows:
	Stringent complete response (CR): CR with normalization of serum‐free light chains
	CR: No detectable monoclonal protein by serum protein electrophoresis and immunofixation
	Very good partial response (PR): 90% improvement in M‐protein
	PR: 50% improvement in M‐protein
	Minimal response: 25% improvement in M‐protein
	Stable disease (SD): Neither PR nor progressive disease (PD)
	PD: 25% increase in M‐protein
	In terms of ORR, a PR or better is required. In cases with light chain–only disease, the difference between involved and uninvolved light chains is used to gauge response.
Investigator's Analysis	Study prematurely terminated because of the COVID‐19 pandemic, with only modest activity in a small number of patients.

Generic Name	Avelumab
Trade Name	Bavencio
Company Name	EMD Serono, Inc.
Drug Type	Antibody
Drug Class	Immune therapy
Dose	800 milligrams (mg) per flat dose
Route	i.v.
Schedule of Administration	Days 1 and 15 of every cycle (28‐day cycles) until progression or intolerable toxicity
Generic Name	Hypofractionated radiation therapy
Schedule of Administration	Patients received focal hypofractionated radiation therapy on cycle 2 days 1–5 at a goal strategy of 5 Gy daily for 5 days (adjusted at the radiation oncologist's discretion).

Number of Patients, Male	2
Number of Patients, Female	2
Stage	Revised International Staging System for Myeloma: stage 1: 4 (100%)
Age	Median (range): 68 (62–83), years
Number of prior systemic therapies	Median (range): 3 (2–4)
Performance Status: ECOG	0 —— 2 (50%) 1 —— 2 (50%)
Cytogenetics, n (%)	Normal: 2 (50%) Hyperdiploidy: 1 (25%) Deletion 13q: 1 (25%)
Immunoglobulin isotype, n (%)	IgG Kappa: 2 (50%) IgG Lambda: 1 (25%) IgD Kappa: 1 (25%)

Title	Response Rate
Number of Patients Screened	9
Number of Patients Enrolled	4
Number of Patients Evaluable for Toxicity	4
Number of Patients Evaluated for Efficacy	4
Evaluation Method	IMWG Response Criteria
Response Assessment SD	n = 2 (50%)
Response Assessment PD	n = 1 (25%)
Response Assessment OTHER	n = 1 (25%)
(Median) Duration Assessments PFS	5.3 months, CI: 2.5–7.1
Waterfall plot (Figure 2 )	Waterfall plot of best percent change in M‐protein values and IMWG response. One patient had disease progression; two had stable disease, and one had a minimal response, based on M‐protein. There were no partial responses.
Outcome Notes	The 2014 IMWG response criteria for multiple myeloma were used.

All Cycles
Name	NC/NA, %	Grade 1, %	Grade 2, %	Grade 3, %	Grade 4, %	Grade 5, %	All grades, %
Alanine aminotransferase increased	75	0	25	0	0	0	25
Aspartate aminotransferase increased	75	0	25	0	0	0	25
Pain	75	25	0	0	0	0	25
Arthralgia	75	25	0	0	0	0	25
Rash maculopapular	75	25	0	0	0	0	25
Adverse Events Legend
All‐grade treatment‐emergent adverse events occurring during any cycle

Abbreviation: NC/NA, no change from baseline/no adverse event.

Completion	Did not fully accrue; study terminated before completion
Investigator's Assessment	Study prematurely terminated because of the COVID‐19, with only modest activity in a small number of patients.