Yeasiry Corrales1, Elizabeth Ferrer2,3, José Fernández1,4,5, José Gauta6, Marielena García7, Aixa Aguirre7, Arlett Pérez7. 1. Facultad de Ciencias de la Salud, Departamento de Parasitología, Universidad de Carabobo Sede Aragua, Maracay, Estado Aragua, Venezuela. 2. Facultad de Ciencias de la Salud, Departamento de Parasitología, Universidad de Carabobo Sede Aragua, Maracay, Estado Aragua, Venezuela. elizabeth.ferrer@gmail.com. 3. Facultad de Ciencias de la Salud, Instituto de Investigaciones Biomédicas "Dr, Francisco J. Triana Alonso", Universidad de Carabobo Sede Aragua, Maracay, Estado Aragua, Venezuela. elizabeth.ferrer@gmail.com. 4. Facultad de Ciencias de la Salud, Instituto de Investigaciones Biomédicas "Dr, Francisco J. Triana Alonso", Universidad de Carabobo Sede Aragua, Maracay, Estado Aragua, Venezuela. 5. Centro Nacional de Investigaciones Agrícolas y Pecuarias (CENIAP), Instituto Nacional de Investigaciones Agrícolas (INIA), Ministerio del Poder Popular Para la Producción Agrícola (MPPPA, Maracay, Estado Aragua, Venezuela. 6. Cátedra de Sanidad Animal (Agrotecnia), Instituto Universitario Tecnológico de Ejido (IUTE) Extensión Bailadores, Bailadores, Estado Mérida, Venezuela. 7. Facultad de Ciencias Veterinarias, Cátedra de Parasitología Veterinaria, Universidad Central de Venezuela (UCV-FCV), Maracay, Estado Aragua, Venezuela.
Abstract
INTRODUCTION: Fascioliasis is caused in Venezuela by the trematode Fasciola hepatica, affecting herbivorous and human. The Venezuelan Andean region is endemic for bovine fascioliasis and its presence in humans is not known. The objective of this work was to detected positive cases of bovine and human fascioliasis by coprological and immunological techniques and determine the possible risk factors, in eight cattle farms of a Venezuelan Andean rural area. METHODS: We studied 143 samples of feces and sera of bovines, and 34 samples of feces and sera of humans. Feces were examined by several coprologic techniques, while sera were evaluated by ELISA using two antigens: crude extract (CE) and surface proteins (SP) of F. hepatica, which were previously standardized and validated. RESULTS: The frequency of fascioliasis in bovines was 21% by coprology, and 49.7% by SP-ELISA. The human detection was 0% by coprology, and 29.4% by SP-ELISA. There were statistical significative differences between cattle farms, regarding to the positive results by coprology and by SP-ELISA. About the possible risk factors, statistical association was found only with the presence of snails near or in the farms and consumption of non-channeled water (river, ditch or spring), both for cattle and for humans. CONCLUSION: The results showed that the studied area is endemic for bovine fascioliasis, the human has been in contact with F. hepatica and there are risk factors for the transmission of the parasite in the studied farms.
INTRODUCTION:Fascioliasis is caused in Venezuela by the trematode Fasciola hepatica, affecting herbivorous and human. The Venezuelan Andean region is endemic for bovinefascioliasis and its presence in humans is not known. The objective of this work was to detected positive cases of bovine and humanfascioliasis by coprological and immunological techniques and determine the possible risk factors, in eight cattle farms of a Venezuelan Andean rural area. METHODS: We studied 143 samples of feces and sera of bovines, and 34 samples of feces and sera of humans. Feces were examined by several coprologic techniques, while sera were evaluated by ELISA using two antigens: crude extract (CE) and surface proteins (SP) of F. hepatica, which were previously standardized and validated. RESULTS: The frequency of fascioliasis in bovines was 21% by coprology, and 49.7% by SP-ELISA. The human detection was 0% by coprology, and 29.4% by SP-ELISA. There were statistical significative differences between cattle farms, regarding to the positive results by coprology and by SP-ELISA. About the possible risk factors, statistical association was found only with the presence of snails near or in the farms and consumption of non-channeled water (river, ditch or spring), both for cattle and for humans. CONCLUSION: The results showed that the studied area is endemic for bovinefascioliasis, the human has been in contact with F. hepatica and there are risk factors for the transmission of the parasite in the studied farms.