Background: Most biomedical research has focused on sampling COVID-19 patients presenting to hospital with advanced disease, with less focus on the asymptomatic or paucisymptomatic. We established a bioresource with serial sampling of health care workers (HCWs) designed to obtain samples before and during mainly mild disease, with follow-up sampling to evaluate the quality and duration of immune memory. Methods: We conducted a prospective study on HCWs from three hospital sites in London, initially at a single centre (recruited just prior to first peak community transmission in London), but then extended to multiple sites 3 weeks later (recruitment still ongoing, target n=1,000). Asymptomatic participants attending work complete a health questionnaire, and provide a nasal swab (for SARS-CoV-2 RNA by RT-PCR tests) and blood samples (mononuclear cells, serum, plasma, RNA and DNA are biobanked) at 16 weekly study visits, and at 6 and 12 months. Results: Preliminary baseline results for the first 731 HCWs (400 single-centre, 331 multicentre extension) are presented. Mean age was 38±11 years; 67% are female, 31% nurses, 20% doctors, and 19% work in intensive care units. COVID-19-associated risk factors were: 37% black, Asian or minority ethnicities; 18% smokers; 13% obesity; 11% asthma; 7% hypertension and 2% diabetes mellitus. At baseline, 41% reported symptoms in the preceding 2 weeks. Preliminary test results from the initial cohort (n=400) are available: PCR at baseline for SARS-CoV-2 was positive in 28 of 396 (7.1%, 95% CI 4.9-10.0%) and 15 of 385 (3.9%, 2.4-6.3%) had circulating IgG antibodies. Conclusions: This COVID-19 bioresource established just before the peak of infections in the UK will provide longitudinal assessments of incident infection and immune responses in HCWs through the natural time course of disease and convalescence. The samples and data from this bioresource are available to academic collaborators by application https://covid-consortium.com/application-for-samples/. Copyright:
Background: Most biomedical research has focused on sampling COVID-19patients presenting to hospital with advanced disease, with less focus on the asymptomatic or paucisymptomatic. We established a bioresource with serial sampling of health care workers (HCWs) designed to obtain samples before and during mainly mild disease, with follow-up sampling to evaluate the quality and duration of immune memory. Methods: We conducted a prospective study on HCWs from three hospital sites in London, initially at a single centre (recruited just prior to first peak community transmission in London), but then extended to multiple sites 3 weeks later (recruitment still ongoing, target n=1,000). Asymptomatic participants attending work complete a health questionnaire, and provide a nasal swab (for SARS-CoV-2 RNA by RT-PCR tests) and blood samples (mononuclear cells, serum, plasma, RNA and DNA are biobanked) at 16 weekly study visits, and at 6 and 12 months. Results: Preliminary baseline results for the first 731 HCWs (400 single-centre, 331 multicentre extension) are presented. Mean age was 38±11 years; 67% are female, 31% nurses, 20% doctors, and 19% work in intensive care units. COVID-19-associated risk factors were: 37% black, Asian or minority ethnicities; 18% smokers; 13% obesity; 11% asthma; 7% hypertension and 2% diabetes mellitus. At baseline, 41% reported symptoms in the preceding 2 weeks. Preliminary test results from the initial cohort (n=400) are available: PCR at baseline for SARS-CoV-2 was positive in 28 of 396 (7.1%, 95% CI 4.9-10.0%) and 15 of 385 (3.9%, 2.4-6.3%) had circulating IgG antibodies. Conclusions: This COVID-19 bioresource established just before the peak of infections in the UK will provide longitudinal assessments of incident infection and immune responses in HCWs through the natural time course of disease and convalescence. The samples and data from this bioresource are available to academic collaborators by application https://covid-consortium.com/application-for-samples/. Copyright:
Authors: Paul A Harris; Robert Taylor; Brenda L Minor; Veida Elliott; Michelle Fernandez; Lindsay O'Neal; Laura McLeod; Giovanni Delacqua; Francesco Delacqua; Jacqueline Kirby; Stephany N Duda Journal: J Biomed Inform Date: 2019-05-09 Impact factor: 6.317
Authors: Benjamin Jean Gaborit; Jean-François Bergmann; Cristina Mussini; Jose Ramon Arribas; Georg Behrens; Sharon Walmsley; Anton Pozniak; François Raffi Journal: Lancet Infect Dis Date: 2020-04-21 Impact factor: 25.071
Authors: Melissa M Arons; Kelly M Hatfield; Sujan C Reddy; Anne Kimball; Allison James; Jesica R Jacobs; Joanne Taylor; Kevin Spicer; Ana C Bardossy; Lisa P Oakley; Sukarma Tanwar; Jonathan W Dyal; Josh Harney; Zeshan Chisty; Jeneita M Bell; Mark Methner; Prabasaj Paul; Christina M Carlson; Heather P McLaughlin; Natalie Thornburg; Suxiang Tong; Azaibi Tamin; Ying Tao; Anna Uehara; Jennifer Harcourt; Shauna Clark; Claire Brostrom-Smith; Libby C Page; Meagan Kay; James Lewis; Patty Montgomery; Nimalie D Stone; Thomas A Clark; Margaret A Honein; Jeffrey S Duchin; John A Jernigan Journal: N Engl J Med Date: 2020-04-24 Impact factor: 91.245
Authors: Kathleen G Beavis; Scott M Matushek; Ana Precy F Abeleda; Cindy Bethel; Carlissa Hunt; Stephanie Gillen; Angelica Moran; Vera Tesic Journal: J Clin Virol Date: 2020-05-23 Impact factor: 3.168
Authors: Emily S Barrett; Daniel B Horton; Jason Roy; Maria Laura Gennaro; Andrew Brooks; Jay Tischfield; Patricia Greenberg; Tracy Andrews; Sugeet Jagpal; Nancy Reilly; Jeffrey L Carson; Martin J Blaser; Reynold A Panettieri Journal: BMC Infect Dis Date: 2020-11-16 Impact factor: 3.090
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Authors: Stuart Astbury; Catherine J Reynolds; David K Butler; Diana C Muñoz-Sandoval; Kai-Min Lin; Franziska P Pieper; Ashley Otter; Afroditi Kouraki; Lola Cusin; Jessica Nightingale; Amrita Vijay; Simon Craxford; Guruprasad P Aithal; Patrick J Tighe; Joseph M Gibbons; Corinna Pade; George Joy; Mala Maini; Benny Chain; Amanda Semper; Timothy Brooks; Benjamin J Ollivere; Áine McKnight; Mahdad Noursadeghi; Thomas A Treibel; Charlotte Manisty; James C Moon; Ana M Valdes; Rosemary J Boyton; Daniel M Altmann Journal: Immunology Date: 2022-03-08 Impact factor: 7.215
Authors: Gabriella Captur; James C Moon; Constantin-Cristian Topriceanu; George Joy; Leo Swadling; Jenny Hallqvist; Ivan Doykov; Nina Patel; Justyna Spiewak; Tomas Baldwin; Matt Hamblin; Katia Menacho; Marianna Fontana; Thomas A Treibel; Charlotte Manisty; Ben O'Brien; Joseph M Gibbons; Corrina Pade; Tim Brooks; Daniel M Altmann; Rosemary J Boyton; Áine McKnight; Mala K Maini; Mahdad Noursadeghi; Kevin Mills; Wendy E Heywood Journal: EBioMedicine Date: 2022-09-27 Impact factor: 11.205
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Authors: Rishi K Gupta; Joshua Rosenheim; Lucy C Bell; Aneesh Chandran; Jose A Guerra-Assuncao; Gabriele Pollara; Matthew Whelan; Jessica Artico; George Joy; Hibba Kurdi; Daniel M Altmann; Rosemary J Boyton; Mala K Maini; Aine McKnight; Jonathan Lambourne; Teresa Cutino-Moguel; Charlotte Manisty; Thomas A Treibel; James C Moon; Benjamin M Chain; Mahdad Noursadeghi Journal: Lancet Microbe Date: 2021-07-06