Ricardo Pereira Nogueira1,2, Jose Deuzimar Uchoa3,4, Fanny Hilario2, Gabriela de Fátima Santana-Melo5, Luana Marotta Reis de Vasconcellos5, Fernanda Roberta Marciano6, Virginie Roche2, Alberto Moreira Jorge Junior2,7, Anderson Oliveira Lobo4. 1. Chemical Engineering Department, Khalifa University of Science and Technology, Abu Dhabi 127788, United Arab Emirates. 2. Université Grenoble Alpes, Université Savoie Mont Blanc, CNRS, GrenobleINPLEPMI, Grenoble 38000, France. 3. Federal Institute of Education, Science and Technology of Piauí, Teresina 64053-390, Brazil. 4. Interdisciplinary Laboratory for Advanced Materials, BioMatLab Group, Materials Science and Engineering Graduate Program, UFPI - Federal University of Piaui, Teresina 64049-550 Brazil. 5. Department of Bioscience and Oral Diagnosis, Institute of Science and Technology, Sao Paulo State University, Sao José dos Campos 12245-000, Brazil. 6. Department of Physics, Federal University of Piaui, Teresina PI 64049-550 Brazil. 7. Department of Materials Engineering, Federal University of São Carlos, São Carlos 13565-905, Brazil.
Abstract
BACKGROUND: Nanostructured surface modifications of Ti-based biomaterials are moving up from a highly-promising to a successfully-implemented approach to developing safe and reliable implants. METHODS: The study's main objective is to help consolidate the knowledge and identify the more suitable experimental strategies related to TiO2 nanotubes-modified surfaces. In this sense, it proposes the thorough investigation of two optimized nanotubes morphologies in terms of their biological activity (cell cytotoxicity, alkaline phosphatase activity, alizarin red mineralization test, and cellular adhesion) and their electrochemical behavior in simulated body fluid (SBF) electrolyte. Layers of small-short and large-long nanotubes were prepared and investigated in their amorphous and crystallized states and compared to non-anodized samples. RESULTS: Results show that much more than the surface area development associated with the nanotubes' growth; it is the heat treatment-induced change from amorphous to crystalline anatase-rutile structures that ensure enhanced biological activity coupled to high corrosion resistance. CONCLUSION: Compared to both non-anodized and amorphous nanotubes layers, the crystallized nano-structures' outstanding bioactivity was related to the remarkable increase in their hydrophilic behavior, while the enhanced electrochemical stability was ascribed to the thickening of the dense rutile barrier layer at the Ti surface beneath the nanotubes.
BACKGROUND: Nanostructured surface modifications of Ti-based biomaterials are moving up from a highly-promising to a successfully-implemented approach to developing safe and reliable implants. METHODS: The study's main objective is to help consolidate the knowledge and identify the more suitable experimental strategies related to TiO2 nanotubes-modified surfaces. In this sense, it proposes the thorough investigation of two optimized nanotubes morphologies in terms of their biological activity (cell cytotoxicity, alkaline phosphatase activity, alizarin red mineralization test, and cellular adhesion) and their electrochemical behavior in simulated body fluid (SBF) electrolyte. Layers of small-short and large-long nanotubes were prepared and investigated in their amorphous and crystallized states and compared to non-anodized samples. RESULTS: Results show that much more than the surface area development associated with the nanotubes' growth; it is the heat treatment-induced change from amorphous to crystalline anatase-rutile structures that ensure enhanced biological activity coupled to high corrosion resistance. CONCLUSION: Compared to both non-anodized and amorphous nanotubes layers, the crystallized nano-structures' outstanding bioactivity was related to the remarkable increase in their hydrophilic behavior, while the enhanced electrochemical stability was ascribed to the thickening of the dense rutile barrier layer at the Ti surface beneath the nanotubes.
Authors: Gabriela F Santana-Melo; Bruno V M Rodrigues; Edmundo da Silva; Ritchelli Ricci; Fernanda R Marciano; Thomas J Webster; Luana M R Vasconcellos; Anderson O Lobo Journal: Colloids Surf B Biointerfaces Date: 2017-04-27 Impact factor: 5.268
Authors: Jung Park; Sebastian Bauer; Andreas Pittrof; Manuela S Killian; Patrik Schmuki; Klaus von der Mark Journal: Small Date: 2011-11-18 Impact factor: 13.281
Authors: V C Anitha; Jin-Hyung Lee; Jintae Lee; Arghya Narayan Banerjee; Sang Woo Joo; Bong Ki Min Journal: Nanotechnology Date: 2015-01-21 Impact factor: 3.874
Authors: Renata Falchete do Prado; Gabriela Campos Esteves; Evelyn Luzia De Souza Santos; Daiane Acácia Griti Bueno; Carlos Alberto Alves Cairo; Luis Gustavo Oliveira De Vasconcellos; Renata Silveira Sagnori; Fernanda Bastos Pereira Tessarin; Felipe Eduardo Oliveira; Luciane Dias De Oliveira; Maria Fernanda Lima Villaça-Carvalho; Vinicius André Rodrigues Henriques; Yasmin Rodarte Carvalho; Luana Marotta Reis De Vasconcellos Journal: PLoS One Date: 2018-05-17 Impact factor: 3.240