Yun Hu1, Yanyan Li2, Xiaoqiong Yang1, Chunli Li1, Lin Wang3, Ji Feng2, Shouwen Chen1, Xihong Li4, Yong Yang5. 1. State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Science, Hubei University, Wuhan, 430062, China. 2. Tobacco Research Institute of Hubei Province, Wuhan, 430030, China. 3. Hubei Tobacco Industry Co., Ltd., Wuhan, 430040, China. 4. Tobacco Research Institute of Hubei Province, Wuhan, 430030, China. lxh885@126.com. 5. State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Science, Hubei University, Wuhan, 430062, China. yangyong@hubu.edu.cn.
Abstract
Bacterial wilt as a soil-borne disease was caused by Ralstonia solanacearum, and seriously damages the growth of tobacco. Integrated biocontrol method was explored to control bacterial wilt. Nevertheless, the long-term effects of the integrated biocontrol method on soil bacterial community, soil physicochemical properties and the incidence of bacterial wilt are not well understood. In this study, B. amyoliquefaciens ZM9, calcium cyanamide and rice bran were applied to tobacco fields in different ways. The disease index and incidence of tobacco bacterial wilt (TBW), soil physicochemical properties, colonization ability of B. amyoliquefaciens ZM9, and rhizopshere bacterial community were investigated. The results showed that the integrated application of B. amyoliquefaciens ZM9, rice bran and calcium cyanamide had the highest control efficiency of TBW and bacteria community diversity. Additionally, the integrated biocontrol method could improve the colonization ability of B. amyoliquefaciens ZM9. Furthermore, the integrated biocontrol method could effectively suppress TBW by regulating soil physicochemical properties, promoting beneficial bacteria and antagonistic bacteria of rhizopshere soil. This strategy has prospect of overcoming the defects in application of a single antagonistic bacteria and provides new insights to understand how to improve the colonization capacity of antagonistic bacteria and control efficacy for TBW.
Bacterial wilt as a soil-borne disease was caused by pan> class="Species">Ralstonia solanacearum, and seriously damages the growth of tobacco. Integrated biocontrol method was explored to control bacterial wilt. Nevertheless, the long-term effects of the integrated biocontrol method on soil bacterial community, soil physicochemical properties and the incidence of bacterial wilt are not well understood. In this study, B. amyoliquefaciens ZM9, calcium cyanamide and rice bran were applied to tobacco fields in different ways. The disease index and incidence of tobacco bacterial wilt (TBW), soil physicochemical properties, colonization ability of B. amyoliquefaciens ZM9, and rhizopshere bacterial community were investigated. The results showed that the integrated application of B. amyoliquefaciens ZM9, rice bran and calcium cyanamide had the highest control efficiency of TBW and bacteria community diversity. Additionally, the integrated biocontrol method could improve the colonization ability of B. amyoliquefaciens ZM9. Furthermore, the integrated biocontrol method could effectively suppress TBW by regulating soil physicochemical properties, promoting beneficial bacteria and antagonistic bacteria of rhizopshere soil. This strategy has prospect of overcoming the defects in application of a single antagonistic bacteria and provides new insights to understand how to improve the colonization capacity of antagonistic bacteria and control efficacy for TBW.
Authors: Jonathan W Leff; Stuart E Jones; Suzanne M Prober; Albert Barberán; Elizabeth T Borer; Jennifer L Firn; W Stanley Harpole; Sarah E Hobbie; Kirsten S Hofmockel; Johannes M H Knops; Rebecca L McCulley; Kimberly La Pierre; Anita C Risch; Eric W Seabloom; Martin Schütz; Christopher Steenbock; Carly J Stevens; Noah Fierer Journal: Proc Natl Acad Sci U S A Date: 2015-08-17 Impact factor: 11.205