Melquizedec Luiz Silva Pinheiro1, Cleusa Yoshiko Nagamachi1, Talita Fernanda Augusto Ribas1, Cristovam Guerreiro Diniz2, Patricia Caroline Mary O Brien3, Malcolm Andrew Ferguson-Smith3, Fengtang Yang4, Julio Cesar Pieczarka5. 1. Laboratório de Citogenética, Centro de Estudos Avançados da Biodiversidade, ICB, Universidade Federal do Pará, PCT-Guamá, Terreno 11, Belém, Pará, 66075-750, Brazil. 2. Laboratório de Biologia Molecular e Neuroecologia, Instituto Federal do Pará, Campus de Bragança, Avenida dos Bragançanos s/nº, Bragança, Pará, 68600-000, Brazil. 3. Department of Veterinary Medicine, Cambridge Resource Centre for Comparative Genomics, University of Cambridge, Cambridge, CB3 0ES, UK. 4. Cytogenetics Facility, Wellcome Trust Sanger Institute, Hinxton, CB10 1SA, Cambridgeshire, UK. 5. Laboratório de Citogenética, Centro de Estudos Avançados da Biodiversidade, ICB, Universidade Federal do Pará, PCT-Guamá, Terreno 11, Belém, Pará, 66075-750, Brazil. juliopieczarka@gmail.com.
Abstract
BACKGROUND: The Scolopacidae family (Suborder Scolopaci, Charadriiformes) is composed of sandpipers and snipes; these birds are long-distance migrants that show great diversity in their behavior and habitat use. Cytogenetic studies in the Scolopacidae family show the highest diploid numbers for order Charadriiformes. This work analyzes for the first time the karyotype of Actitis macularius by classic cytogenetics and chromosome painting. RESULTS: The species has a diploid number of 92, composed mostly of telocentric pairs. This high 2n is greater than the proposed 80 for the avian ancestral putative karyotype (a common feature among Scolopaci), suggesting that fission rearrangements have formed smaller macrochromosomes and microchromosomes. Fluorescence in situ hybridization using Burhinus oedicnemus whole chromosome probes confirmed the fissions in pairs 1, 2, 3, 4 and 6 of macrochromosomes. CONCLUSION: Comparative analysis with other species of Charadriiformes studied by chromosome painting together with the molecular phylogenies for the order allowed us to raise hypotheses about the chromosomal evolution in suborder Scolopaci. From this, we can establish a clear idea of how chromosomal evolution occurred in this suborder.
BACKGROUND: The Scolopacidae family (Suborder Scolopaci, Charadriiformes) is composed of sandpipers and snipes; these birds are long-distance migrants that show great diversity in their behavior and habitat use. Cytogenetic studies in the Scolopacidae family show the highest diploid numbers for order Charadriiformes. This work analyzes for the first time the karyotype of Actitis macularius by classic cytogenetics and chromosome painting. RESULTS: The species has a diploid number of 92, composed mostly of telocentric pairs. This high 2n is greater than the proposed 80 for the avian ancestral putative karyotype (a common feature among Scolopaci), suggesting that fission rearrangements have formed smaller macrochromosomes and microchromosomes. Fluorescence in situ hybridization using Burhinus oedicnemus whole chromosome probes confirmed the fissions in pairs 1, 2, 3, 4 and 6 of macrochromosomes. CONCLUSION: Comparative analysis with other species of Charadriiformes studied by chromosome painting together with the molecular phylogenies for the order allowed us to raise hypotheses about the chromosomal evolution in suborder Scolopaci. From this, we can establish a clear idea of how chromosomal evolution occurred in this suborder.
Entities:
Keywords:
Burhinus oedicnemus; Chromosomal evolution; Classic and molecular cytogenetics; Phylogeny
Authors: Guojie Zhang; Cai Li; Qiye Li; Bo Li; Denis M Larkin; Chul Lee; Jay F Storz; Agostinho Antunes; Matthew J Greenwold; Robert W Meredith; Anders Ödeen; Jie Cui; Qi Zhou; Luohao Xu; Hailin Pan; Zongji Wang; Lijun Jin; Pei Zhang; Haofu Hu; Wei Yang; Jiang Hu; Jin Xiao; Zhikai Yang; Yang Liu; Qiaolin Xie; Hao Yu; Jinmin Lian; Ping Wen; Fang Zhang; Hui Li; Yongli Zeng; Zijun Xiong; Shiping Liu; Long Zhou; Zhiyong Huang; Na An; Jie Wang; Qiumei Zheng; Yingqi Xiong; Guangbiao Wang; Bo Wang; Jingjing Wang; Yu Fan; Rute R da Fonseca; Alonzo Alfaro-Núñez; Mikkel Schubert; Ludovic Orlando; Tobias Mourier; Jason T Howard; Ganeshkumar Ganapathy; Andreas Pfenning; Osceola Whitney; Miriam V Rivas; Erina Hara; Julia Smith; Marta Farré; Jitendra Narayan; Gancho Slavov; Michael N Romanov; Rui Borges; João Paulo Machado; Imran Khan; Mark S Springer; John Gatesy; Federico G Hoffmann; Juan C Opazo; Olle Håstad; Roger H Sawyer; Heebal Kim; Kyu-Won Kim; Hyeon Jeong Kim; Seoae Cho; Ning Li; Yinhua Huang; Michael W Bruford; Xiangjiang Zhan; Andrew Dixon; Mads F Bertelsen; Elizabeth Derryberry; Wesley Warren; Richard K Wilson; Shengbin Li; David A Ray; Richard E Green; Stephen J O'Brien; Darren Griffin; Warren E Johnson; David Haussler; Oliver A Ryder; Eske Willerslev; Gary R Graves; Per Alström; Jon Fjeldså; David P Mindell; Scott V Edwards; Edward L Braun; Carsten Rahbek; David W Burt; Peter Houde; Yong Zhang; Huanming Yang; Jian Wang; Erich D Jarvis; M Thomas P Gilbert; Jun Wang Journal: Science Date: 2014-12-11 Impact factor: 47.728
Authors: Rafael Kretschmer; Ricardo J Gunski; Analía del Valle Garnero; Patricia C M O'Brien; Malcolm A Ferguson-Smith; Thales R Ochotorena de Freitas; Edivaldo H Correa de Oliveira Journal: Cytogenet Genome Res Date: 2015-06-16 Impact factor: 1.636
Authors: Rafael Kretschmer; Marcelo Santos de Souza; Suziane Alves Barcellos; Tiago Marafiga Degrandi; Jorge C Pereira; Patricia C M O'Brien; Malcolm A Ferguson-Smith; Ricardo José Gunski; Analía Del Valle Garnero; Edivaldo Herculano Correa de Oliveira; Thales Renato Ochotorena de Freitas Journal: Genet Mol Biol Date: 2020-02-17 Impact factor: 1.771