Literature DB >> 3350116

Affinity chromatography of glucose dehydrogenase.

W R Carper1, W C Groutas, D B Coffin.   

Abstract

Porcine liver beta-D-glucose dehydrogenase, a multi-functional protein, has been purified to apparent homogeneity. The enzyme has been separated from the endoplasmic reticulum using Triton X-114 and further purified using NAD to release glucose dehydrogenase from a NADP-linked sepharose column. The purified enzyme is capable of producing both NADH and NADPH in vivo as indicated by kinetic studies.

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Year:  1988        PMID： 3350116     DOI： 10.1007/bf01960233

Source DB:  PubMed          Journal:  Experientia        ISSN： 0014-4754


  12 in total

1.  A possible role for microsomal hexose-6-phosphate dehydrogenase in microsomal electron transport and mixed-function oxygenase activity.

Authors:  J J Stegeman; A V Klotz
Journal:  Biochem Biophys Res Commun       Date:  1979-03-30       Impact factor: 3.575

2.  Intramembraneous localization of rat liver microsomal hexose-6-phosphate dehydrogenase and membrane permeability to its substrates.

Authors:  T Takahashi; S H Hori
Journal:  Biochim Biophys Acta       Date:  1978-06-09

3.  The stimulus-secretion coupling of glucose-induced insulin release. Metabolic effects of menadione in isolated islets.

Authors:  W J Malaisse; J C Hutton; S Kawazu; A Sener
Journal:  Eur J Biochem       Date:  1978-06-01

4.  Bovine liver glucose dehydrogenase: isolation and characterization.

Authors:  D P Campbell; W R Carper; R E Thompson
Journal:  Arch Biochem Biophys       Date:  1982-04-15       Impact factor: 4.013

Review 5.  Insulin release: the fuel hypothesis.

Authors:  W J Malaisse; A Sener; A Herchuelz; J C Hutton
Journal:  Metabolism       Date:  1979-04       Impact factor: 8.694

6.  The stimulus-secretion coupling of glucose-induced insulin release. XXXV. The links between metabolic and cationic events.

Authors:  W J Malaisse; J C Hutton; S Kawazu; A Herchuelz; I Valverde; A Sener
Journal:  Diabetologia       Date:  1979-05       Impact factor: 10.122

7.  Glucose 6-phosphate regulates Ca2+ steady state in endoplasmic reticulum of islets. A possible link in glucose-induced insulin secretion.

Authors:  B A Wolf; J R Colca; P G Comens; J Turk; M L McDaniel
Journal:  J Biol Chem       Date:  1986-12-15       Impact factor: 5.157

8.  General ligands in affinity chromatography. Cofactor-substrate elution of enzymes bound to the immobilized nucleotides adenosine 5'-monophosphate and nicotinamide-adenine dinucleotide.

Authors:  K Mosbach; H Guilford; R Ohlsson; M Scott
Journal:  Biochem J       Date:  1972-05       Impact factor: 3.857

9.  Characterization studies of glucose dehydrogenase.

Authors:  W R Carper; D P Campbell; S W Morrical; R E Thompson
Journal:  Experientia       Date:  1983-11-15

10.  Measurement of protein using bicinchoninic acid.

Authors:  P K Smith; R I Krohn; G T Hermanson; A K Mallia; F H Gartner; M D Provenzano; E K Fujimoto; N M Goeke; B J Olson; D C Klenk
Journal:  Anal Biochem       Date:  1985-10       Impact factor: 3.365
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