Literature DB >> 33496233

Strand-Specific Reverse Transcription PCR for Detection of Replicating SARS-CoV-2.

Catherine A Hogan, ChunHong Huang, Malaya K Sahoo, Hannah Wang, Becky Jiang, Mamdouh Sibai, Marisa Holubar, Roshni Mathew, James Zehnder, Benjamin A Pinsky.   

Abstract

We developed an assay that detects minus-strand RNA as a surrogate for actively replicating severe acute respiratory syndrome coronavirus 2. We detected minus-strand RNA in 41 persons with coronavirus disease up to 30 days after symptom onset. This assay might inform clinical decision-making about patient infectiousness.

Entities:  

Keywords:  2019 novel coronavirus disease; COVID-19; PCR; RNA; SARS-CoV-2; coronavirus; coronavirus disease; diagnosis; infection control; minus strand; respiratory infections; screening; severe acute respiratory syndrome coronavirus 2; testing; viruses; zoonoses

Mesh：

Substances：

Year:  2021        PMID： 33496233     DOI： 10.3201/eid2702.204168

Source DB:  PubMed          Journal:  Emerg Infect Dis        ISSN： 1080-6040            Impact factor:   6.883


  12 in total

Review 1.  Rapid Antigen Assays for SARS-CoV-2: Promise and Peril.

Authors:  Thao T Truong; Jennifer Dien Bard; Susan M Butler-Wu
Journal:  Clin Lab Med       Date:  2022-03-04       Impact factor: 2.172

Review 2.  Novel Assays for Molecular Detection of Severe Acute Respiratory Syndrome Coronavirus 2.

Authors:  Kyle G Rodino; Kenneth P Smith; Matthew A Pettengill
Journal:  Clin Lab Med       Date:  2022-03-09       Impact factor: 2.172

Review 3.  The Successes and Challenges of SARS-CoV-2 Molecular Testing in the United States.

Authors:  Jennifer Dien Bard; N Esther Babady
Journal:  Clin Lab Med       Date:  2022-03-08       Impact factor: 2.172

4.  Characterization of SARS-CoV-2 and host entry factors distribution in a COVID-19 autopsy series.

Authors:  Xiao-Ming Wang; Rahul Mannan; Arul M Chinnaiyan; Rohit Mehra; Lanbo Xiao; Eman Abdulfatah; Yuanyuan Qiao; Carol Farver; Jeffrey L Myers; Sylvia Zelenka-Wang; Lisa McMurry; Fengyun Su; Rui Wang; Liron Pantanowitz; Jeffrey Jentzen; Allecia Wilson; Yuping Zhang; Xuhong Cao
Journal:  Commun Med (Lond)       Date:  2021-08-23

5.  Persistent SARS-CoV-2 infection and increasing viral variants in children and young adults with impaired humoral immunity.

Authors:  Thao T Truong; Alex Ryutov; Utsav Pandey; Rebecca Yee; Lior Goldberg; Deepa Bhojwani; Paibel Aguayo-Hiraldo; Benjamin A Pinsky; Andrew Pekosz; Lishuang Shen; Scott D Boyd; Oliver F Wirz; Katharina Röltgen; Moiz Bootwalla; Dennis T Maglinte; Dejerianne Ostrow; David Ruble; Jennifer H Han; Jaclyn A Biegel; Maggie Li; ChunHong Huang; Mayala K Sahoo; Pia S Pannaraj; Maurice O'Gorman; Alexander R Judkins; Xiaowu Gai; Jennifer Dien Bard
Journal:  medRxiv       Date:  2021-03-02

6.  Predicting infectivity: comparing four PCR-based assays to detect culturable SARS-CoV-2 in clinical samples.

Authors:  Emily A Bruce; Margaret G Mills; Reigran Sampoleo; Garrett A Perchetti; Meei-Li Huang; Hannah W Despres; Madaline M Schmidt; Pavitra Roychoudhury; David J Shirley; Keith R Jerome; Alexander L Greninger; Jason W Botten
Journal:  EMBO Mol Med       Date:  2021-12-13       Impact factor: 14.260

7.  SARS-CoV-2 Subgenomic RNA Kinetics in Longitudinal Clinical Samples.

Authors:  Renu Verma; Eugene Kim; Giovanny Joel Martínez-Colón; Prasanna Jagannathan; Arjun Rustagi; Julie Parsonnet; Hector Bonilla; Chaitan Khosla; Marisa Holubar; Aruna Subramanian; Upinder Singh; Yvonne Maldonado; Catherine A Blish; Jason R Andrews
Journal:  Open Forum Infect Dis       Date:  2021-06-11       Impact factor: 4.423

Review 8.  SARS-CoV-2 Subgenomic RNAs: Characterization, Utility, and Perspectives.

Authors:  Samuel Long
Journal:  Viruses       Date:  2021-09-24       Impact factor: 5.818

9.  Novel utilization of strand-specific reverse transcription polymerase chain reaction in perioperative clinical decision making for SARS-CoV-2 polymerase chain reaction positive patients.

Authors:  Christine G Jette; Tammy Wang; Ellen Wang; Janice Y Man; Samuel Mireles; Birgit Maass; Roshni Mathew; Benjamin A Pinsky; Rebecca E Claure; Genevieve D'Souza
Journal:  Paediatr Anaesth       Date:  2022-04-05       Impact factor: 2.129

10.  SARS-CoV-2 Neutralization Resistance Mutations in Patient with HIV/AIDS, California, USA.

Authors:  Seth A Hoffman; Cristina Costales; Malaya K Sahoo; Srikanth Palanisamy; Fumiko Yamamoto; ChunHong Huang; Michelle Verghese; Daniel A Solis; Mamdouh Sibai; Aruna Subramanian; Lucy S Tompkins; Philip Grant; Robert W Shafer; Benjamin A Pinsky
Journal:  Emerg Infect Dis       Date:  2021-07-23       Impact factor: 6.883
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