Sadra Mohaghegh1, Hossein Mohammad-Rahimi2, Ladan Eslamian3, Asghar Ebadifar4, Mohammad Reza Badiee5, Mohammadhossein Farahani6, Masoud Mohebbi Rad2, Saeed Reza Motamedian5,7. 1. Undergraduate Student, Student Research Committee, School of Dentistry, Shahid Beheshti University of Medical Sciences Tehran, Iran. 2. Dental Research Center, Research Institute of Dental Sciences, Shahid Beheshti University of Medical Sciences Tehran, Iran. 3. Professor of Orthodontics, Center of Research & Department of Orthodontics, School of Dentistry, Shahid Beheshti University of Medical Sciences Tehran, Iran. 4. Associated Professor of Orthodontics, Center of Research & Department of Orthodontics, School of Dentistry, Shahid Beheshti University of Medical Sciences Tehran, Iran. 5. Assistant Professor of Orthodontics, Department of Orthodontics, School of Dentistry, Shahid Beheshti University of Medical Sciences Tehran, Iran. 6. Orthodontist Arak, Iran. 7. Dentofacial Deformities Research Center, Research Institute of Dental Sciences, Shahid Beheshti University of Medical Sciences Tehran, Iran.
Abstract
INTRODUCTION: One of the most common orthodontic problems is maxillary constriction, which is mostly treated by rapid palatal expansion (RPE). However, its high rate of relapse and prolonged retention period have led to some challenges for orthodontists. To encounter these issues, accelerating bone regeneration can provide long-term stability of expanded maxilla. The present study aimed to evaluate the effect of low-level laser therapy (LLLT), bone marrow-derived mesenchymal stem cells (BMSCs) and their combination on promoting bone regeneration of the inter-maxillary suture after RPE in rats. MATERIALS AND METHOD: Total of 60 rats went under RPE treatment. After 7 days, retention period started and interventions (group A, Control (saline); group B, LLLT; group C, BMSCs; group D, LLLT + BMSCs) were performed in the sutural area. After 21 days, radiographic and histological analyses were done. Histological analyses were conducted to evaluate the following criteria of the newly formed bone: the number of osteoblasts, new bone formation, vascularization, connective tissue. Moreover, sutural width was assessed in histologic images. To evaluate bone density at suture area, gray scale and Hounsfield Unit values were measured based on the occlusal radiographic and Micro-Computed topography images respectively. RESULTS: Only in group C and D, osteoblasts and new bone formation were observed in all of the samples. There were no significant differences among the study groups regarding the post-treatment sutural width (P > 0.05). In the radiographic analysis, only group D showed more bone density compared to the control group (P = 0.022). Similarly, in micro-CT analysis, the most bone density was observed in group D which was significantly more than the control group (P = 0.013). CONCLUSION: Our findings suggest that the application of LLLT and BMSCs is the most beneficial approach in accelerating bone regeneration in the inter-maxillary suture. AJSC
INTRODUCTION: One of the most common orthodontic problems is maxillary constriction, which is mostly treated by rapid palatal expansion (RPE). However, its high rate of relapse and prolonged retention period have led to some challenges for orthodontists. To encounter these issues, accelerating bone regeneration can provide long-term stability of expanded maxilla. The present study aimed to evaluate the effect of low-level laser therapy (LLLT), bone marrow-derived mesenchymal stem cells (BMSCs) and their combination on promoting bone regeneration of the inter-maxillary suture after RPE in rats. MATERIALS AND METHOD: Total of 60 rats went under RPE treatment. After 7 days, retention period started and interventions (group A, Control (saline); group B, LLLT; group C, BMSCs; group D, LLLT + BMSCs) were performed in the sutural area. After 21 days, radiographic and histological analyses were done. Histological analyses were conducted to evaluate the following criteria of the newly formed bone: the number of osteoblasts, new bone formation, vascularization, connective tissue. Moreover, sutural width was assessed in histologic images. To evaluate bone density at suture area, gray scale and Hounsfield Unit values were measured based on the occlusal radiographic and Micro-Computed topography images respectively. RESULTS: Only in group C and D, osteoblasts and new bone formation were observed in all of the samples. There were no significant differences among the study groups regarding the post-treatment sutural width (P > 0.05). In the radiographic analysis, only group D showed more bone density compared to the control group (P = 0.022). Similarly, in micro-CT analysis, the most bone density was observed in group D which was significantly more than the control group (P = 0.013). CONCLUSION: Our findings suggest that the application of LLLT and BMSCs is the most beneficial approach in accelerating bone regeneration in the inter-maxillary suture. AJSC
Authors: Cristiane Becher Rosa; Fernando Antonio Lima Habib; Telma Martins de Araújo; Jean Nunes Dos Santos; Maria Cristina T Cangussu; Artur Felipe Santos Barbosa; Isabele Cardoso Vieira de Castro; Antônio Luiz Barbosa Pinheiro Journal: Lasers Med Sci Date: 2016-11-24 Impact factor: 3.161