Erdem Toktay1, Jale Selli2, Muhammed Ali Gurbuz3, Tugba Bal Tastan3, Rustem Anıl Ugan4, Harun Un5, Zekai Halici6. 1. Kafkas University, Faculty of Medicine, Department of Histology and Embryology, Kars. 2. Alanya Alaaddin Keykubat University, Faculty of Medicine, Department of Histology and Embryology, Antalya. 3. Ataturk University, Faculty of Medicine, Department of Histology and Embryology, Erzurum. 4. Ataturk University, Faculty of Pharmacy, Department of Pharmacology, Erzurum. 5. Agrı Ibrahim Cecen University, Faculty of Pharmacy, Department of biochemistry, Ağrı. 6. Ataturk University, Faculty of Medicine, Department of Pharmacology, Erzurum.
Abstract
OBJECTIVES: This study aimed to examine the effects of genistein and daidzein on endometrial receptivity by histopathological, immunohistochemical, and biochemical techniques. MATERIALS AND METHODS: In this study, 72 female Sprague-Dawley rats were randomly divided into 8 groups. The endometrial receptivity model was applied to identified groups. Experimental animals were given periorally 10 mg/kg and high 40 mg/kg doses of genistein and daidzein for 5 days by gavage. At the end of the experiment, uterine tissues were evaluated histopathologically, immunohistochemically, and biochemically. RESULTS: When histopathological findings were examined, significant decreases in pinopod formation were observed in high dose genistein and daidzein groups. When compared with the endometrial receptivity group, immunohistochemical staining findings showed a significant decrease in the expression of integrin β3, integrin αvβ3, LIF, and HOXA10 and an increase in MUC 1 expression in the high dose of genistein and daidzein groups. In biochemical evaluations, it was determined that genistein and daidzein increased estrogen levels and decreased progesterone levels in a dose-dependent manner. CONCLUSION: Genistein and daidzein have a negative effect on endometrial receptivity. Therefore, individuals with a risk of infertility should pay attention to the consumption of genistein and daidzein.
OBJECTIVES: This study aimed to examine the effects of genistein and daidzein on endometrial receptivity by histopathological, immunohistochemical, and biochemical techniques. MATERIALS AND METHODS: In this study, 72 female Sprague-Dawley rats were randomly divided into 8 groups. The endometrial receptivity model was applied to identified groups. Experimental animals were given periorally 10 mg/kg and high 40 mg/kg doses of genistein and daidzein for 5 days by gavage. At the end of the experiment, uterine tissues were evaluated histopathologically, immunohistochemically, and biochemically. RESULTS: When histopathological findings were examined, significant decreases in pinopod formation were observed in high dose genistein and daidzein groups. When compared with the endometrial receptivity group, immunohistochemical staining findings showed a significant decrease in the expression of integrin β3, integrin αvβ3, LIF, and HOXA10 and an increase in MUC 1 expression in the high dose of genistein and daidzein groups. In biochemical evaluations, it was determined that genistein and daidzein increased estrogen levels and decreased progesterone levels in a dose-dependent manner. CONCLUSION: Genistein and daidzein have a negative effect on endometrial receptivity. Therefore, individuals with a risk of infertility should pay attention to the consumption of genistein and daidzein.
Authors: Yasemin Çekmez; Vakkas Korkmaz; Aslı Çakır; Ahmet Göçmen; Yusuf Ergün; Serdar Gülşen; Yasam K Akpak Journal: J Matern Fetal Neonatal Med Date: 2015-11-23
Authors: G A Surveyor; S J Gendler; L Pemberton; S K Das; I Chakraborty; J Julian; R A Pimental; C C Wegner; S K Dey; D D Carson Journal: Endocrinology Date: 1995-08 Impact factor: 4.736