Selina Aksak Karamese1, Erdem Toktay2, Deniz Unal2, Jale Selli2, Murat Karamese3, Ismail Malkoc4. 1. Kafkas University, Medical Faculty, Department of Histology and Embryology, 36100 Kars, Turkey. 2. Ataturk University, Medical Faculty, Department of Histology and Embryology, 25240 Erzurum, Turkey. 3. Kafkas University, Medical Faculty, Department of Microbiology, 36100 Kars, Turkey. Electronic address: murat_karamese@hotmail.com. 4. Ataturk University, Medical Faculty, Department of Anatomy, 25240 Erzurum, Turkey.
Abstract
INTRODUCTION: Beta-carotene is a well-known antioxidant and precursor of Vitamin A that has a preventative role in the oxidative damage process. Our aim was to investigate the possible preventive effects of beta-carotene on oxidative damage via experimental ischemia and ischemia-reperfusion models in rat ovaries. MATERIALS AND METHODS: A traumatic vascular clamps were used for 3h to induce ischemia (Group 2, 3, 4, 5, 6, 7). The clamps were then removed to allow reperfusion for 3h (Group 3, 6, 7). Sham-operated rats (Group 1) underwent laparotomy without the induction of ischemia/reperfusion injury. Real-Time-PCR was performed to determine IL-1-beta, IL-6 and iNOS expression levels. Histopathological (H&E) and immunohistochemical staining (NF-kβ p65) processes were then performed. Finally, SOD, GSH, and MDA levels were determined. RESULTS: Intense hemorrhagic areas were observed in both the ischemia and ischemia/reperfusion groups, whereas minimal hemorrhage was observed in the treatment groups. The ischemia and ischemia/reperfusion groups exhibited extreme immunoreactivity, detected by NF-kβ p65 staining; this reactivity decreased after the application of beta-carotene. The expression of IL-1-beta, IL-6, and iNOS in the injury groups increased significantly, whereas a dose-dependent improvement was observed in the treatment groups. Finally, MDA levels increased significantly and SOD and GSH levels decreased drastically in the injury groups. However, these values obtained from I/R groups were normalized after beta-carotene treatment. DISCUSSION: In this study, we demonstrated via molecular and biochemical parameters the protective effect of beta-carotene, which is a potent antioxidant, on the experimental ischemia-reperfusion model.
INTRODUCTION:Beta-carotene is a well-known antioxidant and precursor of Vitamin A that has a preventative role in the oxidative damage process. Our aim was to investigate the possible preventive effects of beta-carotene on oxidative damage via experimental ischemia and ischemia-reperfusion models in ratovaries. MATERIALS AND METHODS: A traumatic vascular clamps were used for 3h to induce ischemia (Group 2, 3, 4, 5, 6, 7). The clamps were then removed to allow reperfusion for 3h (Group 3, 6, 7). Sham-operated rats (Group 1) underwent laparotomy without the induction of ischemia/reperfusion injury. Real-Time-PCR was performed to determine IL-1-beta, IL-6 and iNOS expression levels. Histopathological (H&E) and immunohistochemical staining (NF-kβ p65) processes were then performed. Finally, SOD, GSH, and MDA levels were determined. RESULTS: Intense hemorrhagic areas were observed in both the ischemia and ischemia/reperfusion groups, whereas minimal hemorrhage was observed in the treatment groups. The ischemia and ischemia/reperfusion groups exhibited extreme immunoreactivity, detected by NF-kβ p65 staining; this reactivity decreased after the application of beta-carotene. The expression of IL-1-beta, IL-6, and iNOS in the injury groups increased significantly, whereas a dose-dependent improvement was observed in the treatment groups. Finally, MDA levels increased significantly and SOD and GSH levels decreased drastically in the injury groups. However, these values obtained from I/R groups were normalized after beta-carotene treatment. DISCUSSION: In this study, we demonstrated via molecular and biochemical parameters the protective effect of beta-carotene, which is a potent antioxidant, on the experimental ischemia-reperfusion model.
Authors: Zulkifal Malik; Muzaffar Abbas; Lina Tariq Al Kury; Fawad Ali Shah; Mahboob Alam; Arif-Ullah Khan; Humaira Nadeem; Saad Alghamdi; Muhammad Umar Khayam Sahibzada; Shupeng Li Journal: Drug Des Devel Ther Date: 2021-02-04 Impact factor: 4.162
Authors: Noé M Lopez-Flores; César A Meza-Herrera; Carlos Perez-Marin; Dominique Blache; Gerardo Arellano-Rodríguez; Santiago Zuñiga-Garcia; Cayetano Navarrete-Molina; Cristina García De la Peña; Cesar A Rosales-Nieto; Francisco G Veliz-Deras Journal: Animals (Basel) Date: 2020-04-10 Impact factor: 2.752